Abstract: A near-infrared (NIR) quality monitoring method used in column chromatography for extracting conjugated estrogens (CEs) from pregnant mare urine (PMU), that includes steps of: collecting an eluate obtained from column chromatography of a PMU stock solution as a to-be-tested sample; subjecting the to-be-tested sample to near-infrared spectroscopy (NIRS) to obtain raw spectral data, eliminating abnormal spectral values from the raw spectral data by a Mahalanobis distance method based on L1-PCA, and importing spectral data obtained after the abnormal spectral values are eliminated into a correction model to obtain a CE content in the to-be-tested sample; the correction model is a linear equation illustrating a relationship between true values and measured values, and the measured values refer to the NIR spectral data obtained after the abnormal spectral values are eliminated; and the CEs comprise one or more of sodium 17?-dihydroequilin sulfate, sodium equilin sulfate, and sodium estrone sulfate.

Abstract: The present invention relates to a method for obtaining a natural mixture of conjugated estrogens from urine of pragnant mares (PMU) and use of a macroporous adsorption resin in the method. The method for obtaining a natural mixture of conjugated estrogen from PMU includes the steps of pretreating raw PMU; adsorbing the natural mixture of conjugated estrogens contained in PMU with a macroporous adsorption resin; washing the macroporous adsorption resin laden with the mixture of conjugated estrogens with an alkaline aqueous solution; and eluting the washed adsorption resin with an eluting agent to obtain the mixture of conjugated estrogens. The macroporous adsorption resin is a styrene-divinylbenzene semipolar macroporous adsorption resin with ester group structure. The method according to the invention solves the problems of low adsorptive capacity and high cost existed in the conventional methods, and is suitable for large-scale production.

Abstract: The present invention relates to a method for obtaining a natural mixture of conjugated estrogens from urine of pragnant mares (PMU) and use of a macroporous adsorption resin in the method. The method for obtaining a natural mixture of conjugated estrogen from PMU includes the steps of pretreating raw PMU; adsorbing the natural mixture of conjugated estrogens contained in PMU with a macroporous adsorption resin; washing the macroporous adsorption resin laden with the mixture of conjugated estrogens with an alkaline aqueous solution; and eluting the washed adsorption resin with an eluting agent to obtain the mixture of conjugated estrogens. The macroporous adsorption resin is a styrene-divinylbenzene semipolar macroporous adsorption resin with ester group structure. The method according to the invention solves the problems of low adsorptive capacity and high cost existed in the conventional methods, and is suitable for large-scale production.