Patents by Inventor Xiaoliang Sunney Xie

Xiaoliang Sunney Xie has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11286523
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: January 28, 2020
    Date of Patent: March 29, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Publication number: 20210381045
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: August 24, 2021
    Publication date: December 9, 2021
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Publication number: 20210324469
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: June 30, 2021
    Publication date: October 21, 2021
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Publication number: 20210285038
    Abstract: The present disclosure provides a method for genomic DNA amplification, such as whole genome amplification, including using a transposase system to make fragments of the genomic DNA including primer binding sites, isolating in oil each fragment within its own aqueous microdroplet along with PCR amplification reagents, amplifying each fragment within its own aqueous microdroplet, demulsifying the microdroplets to obtain the amplicons and sequencing the amplicons.
    Type: Application
    Filed: August 31, 2016
    Publication date: September 16, 2021
    Applicant: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Dong Xing, Chi-Han Chang
  • Publication number: 20210277466
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: May 14, 2021
    Publication date: September 9, 2021
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Patent number: 11078533
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: May 12, 2020
    Date of Patent: August 3, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Patent number: 11035001
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: April 14, 2020
    Date of Patent: June 15, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Patent number: 10894980
    Abstract: Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5? end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, forming complementary strands to the single strand
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: January 19, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Chongyi Chen, Dong Xing, Xiaoliang Sunney Xie
  • Publication number: 20200291473
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: April 14, 2020
    Publication date: September 17, 2020
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Publication number: 20200277674
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: May 12, 2020
    Publication date: September 3, 2020
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Publication number: 20200181606
    Abstract: The present disclosure provides a method for amplifying RNA using a combination of reverse transcription and multiple annealing and looping based amplification cycles. Primers are used such that the resulting amplicons include a first cell specific barcode sequence, a second cell specific barcode sequence and a unique molecular identifier barcode sequence.
    Type: Application
    Filed: May 25, 2018
    Publication date: June 11, 2020
    Inventors: Xiaoliang Sunney Xie, Alec R. Chapman, David F. Lee
  • Publication number: 20200157621
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: January 28, 2020
    Publication date: May 21, 2020
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Publication number: 20200102598
    Abstract: The present disclosure provides a method for assembly of genomic DNA using multiplex end-tagging amplification of genomic fragments.
    Type: Application
    Filed: May 23, 2018
    Publication date: April 2, 2020
    Inventors: Xiaoliang Sunney Xie, Dong Xing, Chi-Han Chang, Longzhi Tan
  • Patent number: 10584382
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: March 26, 2019
    Date of Patent: March 10, 2020
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Publication number: 20200063213
    Abstract: The present disclosure provides a method for making an amplified methylome by extending fragments and treating the extended fragments with a methyl transferase and source of methyl groups to transform hemi-methylated double stranded DNA to fully methylated double stranded DNA.
    Type: Application
    Filed: March 8, 2018
    Publication date: February 27, 2020
    Applicant: President and Fellows of Harvard College
    Inventors: Yunlong Cao, Xiaoliang Sunney Xie
  • Publication number: 20190211394
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: March 26, 2019
    Publication date: July 11, 2019
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Publication number: 20190203204
    Abstract: The present disclosure provides a method for de novo assembly of genomic DNA using barcoded fragments.
    Type: Application
    Filed: August 9, 2017
    Publication date: July 4, 2019
    Inventors: Xiaoliang Sunney Xie, Dong Xing, Chi-Han Chang
  • Patent number: 10287630
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: October 11, 2017
    Date of Patent: May 14, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Publication number: 20190002967
    Abstract: Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5? end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, forming complementary strands to the single strand
    Type: Application
    Filed: July 15, 2016
    Publication date: January 3, 2019
    Applicants: President and Fellows of Harvard College, President and Fellows of Harvard College
    Inventors: Chongyi Chen, Dong Xing, Xiaoliang Sunney Xie
  • Patent number: 10065989
    Abstract: The present invention relates to compositions which may comprise a molecular sled linked to cargo and uses thereof. In particular, the present invention relates to a non-naturally occurring or engineered composition which may comprise a molecular sled, linkers and a molecular cargo connected to the sled via the linkers. Methods involving the use of molecular sleds and their cargoes and pharmaceutical compositions, methods for treating cancer, a degenerative disease, a genetic disease or an infectious disease as well as diagnostic methods are also contemplated by the present invention.
    Type: Grant
    Filed: January 13, 2015
    Date of Patent: September 4, 2018
    Assignees: The Broad Institute Inc., President and Fellows of Harvard College, Rijksuniversiteit Groningen
    Inventors: Walter F. Mangel, Paul Blainey, Vito Graziano, Andreas Herrmann, William J. McGrath, Antonius Martinus Van Oijen, Xiaoliang Sunney Xie