Abstract: A filter capable of automatically cutting off gas flow, including: a housing (1) with a cavity, a filter element (2) disposed in the cavity, a blocking mechanism disposed in the flow channel (13), and an unblocking member (23). The housing is provided with an inlet end (11) and an outlet end (12). The filter element (2) is provided with a first chamber (21). A second chamber (23) is formed between the filter element (2) and the housing (1), and a flow channel (13) is disposed between the first chamber (21) and the inlet end (11). The blocking mechanism has an unblocking state in which the blocking mechanism is cooperated with the unblocking member (23) to allow a gas flow to flow out of the flow channel (13), and a blocking state in which the blocking mechanism prevents the gas flow from flowing out of the flow channel (13).

Abstract: A polynucleotide sequencing method includes a wash step that employs a composition including a polymerase. The composition may also include a plurality of nucleotides. The composition may be configured to prevent the polymerase from incorporating one of the plurality of nucleotides into a copy polynucleotide strand.

Abstract: An example of a kit includes a flow cell, a primer fluid, and a cleaving fluid. The flow cell includes at least one surface functionalized with a polymeric hydrogel including azide functional groups or amine functional groups. The primer fluid includes a plurality of alkyne-containing primers, each alkyne-containing primer having an amino cleavable group attaching a primer sequence of the alkyne-containing primer to an alkyne-containing moiety of the alkyne-containing primer. The cleaving fluid includes a substance that is reactive with the amino cleavable group.

Abstract: Embodiments of the present disclosure relate to six-nucleobase libraries having a third Watson-Crick base pair. Also provided herein are methods to prepare such six-nucleobase libraries, and their use for sequencing and modified nucleobase detection applications.

Abstract: 3?-blocked nucleotides, methods of deblocking the same, and methods of synthesizing polynucleotides using the same are provided herein. In some examples, a nucleotide is disposed within the aperture on the first side of a nanopore. The nucleotide may be coupled to a 3?-blocking group including a trigger. The trigger may be selectively activated using an initiator. The activated trigger may be used to remove the 3?-blocking group from the nucleotide.

Abstract: A coalescing filter element, including an inner coalescing component configured to captured a large amount of liquid in gas, and an outer coalescing component configured to coalesce and filter a small amount of liquid remaining in the gas. The inner coalescing component and the outer coalescing component are cylindrical structures disposed in a vertical direction and opened at two ends. The outer coalescing component is sleeved on an outer side of the inner coalescing component, and an annular drainage space is formed between the inner coalescing component and the outer coalescing component. A top end cap is provided on top ends of the inner coalescing component and the outer coalescing component. A bottom end cap is provided on bottom ends of the inner coalescing component and the outer coalescing component. The bottom end cap is provided with a gas inlet communicated with an interior of the inner coalescing component.

Abstract: A polynucleotide sequencing method includes a wash step that employs a composition including a polymerase. The composition may also include a plurality of nucleotides. The composition may be configured to prevent the polymerase from incorporating one of the plurality of nucleotides into a copy polynucleotide strand. The composition may be substantially free of Mg2+.

Abstract: A switchable two-stage coalescence separation system, including a coalescer housing, a plurality of two-stage filter elements, and a particle detector. A lower portion and an upper portion of each of the two-stage filter elements are located in a lower chamber and an upper chamber of the coalescer housing, respectively. Two gas inlet branch pipes are communicated with the lower chamber and the upper chamber, respectively and are connected to a gas inlet main pipe through a first multi-way valve. The particle detector is disposed on the gas inlet main pipe. Two outlet branch pipes are communicated with the lower chamber and the upper chamber, respectively and are connected to a gas outlet main pipe through a second multi-way valve.

Abstract: The present application relates to substituted coumarin derivatives and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

Abstract: There is provided a method, apparatus and system for adapting a machine learning model for optical flow prediction. A machine learning model can be trained or adapted based on compressed video data, using motion vector information extracted from the compressed video data as ground-truth information for use in adapting the model to a motion vector prediction task. The model so adapted can accordingly be adapted for the similar task of optical flow prediction. Thus, the model can be adapted at test time to image data which is taken from an appropriate distribution. A meta-learning process can be performed prior to such model adaptation to potentially improve the model's performance.

Abstract: The present application relates to substituted coumarin derivatives and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

Abstract: The present subject matter relates to reusable flow cells, uses of reusable flow cells, methods of manufacturing a reusable flow cell and methods of regenerating a reusable flow cell.

Abstract: Embodiments of the present disclosure relate to compositions and methods for labeling of nucleotides, oligonucleotides, or polynucleotides with nanoparticles. In particular, labeled nanoparticles can be used to label nucleotides, oligonucleotides, or polynucleotides in sequencing by synthesis.

Abstract: Embodiments of the present disclosure relate to compositions and methods for labeling of nucleotides, oligonucleotides, or polynucleotides with conjugated polymers or polymer dots. In particular, conjugated polymers or polymer dots can be used as detectable labels for nucleotides, oligonucleotides, or polynucleotides in sequencing by synthesis.

Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3? AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.

Abstract: Provided are a method for processing an OFDM signal, a system for transmitting an OFDM signal, and a device, which effectively reduce a peak to average power ratio of the OFDM signal.

Abstract: Embodiments of the present disclosure relate to methods and kits for sequencing by synthesis utilizing biorthogonal chemical reactions between unlabeled nucleotides and post incorporation labeling reagents.

Abstract: Provided herein are methods of determining loss of heterozygosity in human leukocyte antigen in liquid biopsies and applications thereof in cancer treatment.

Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3? AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.

Abstract: Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.