Abstract: Disclosed is a hybridoma cell strain that secretes anti-dinitolmide monoclonal antibodies applicable to the field of food safety immunoassay methods. The hybridoma cell strain DAS3H10 that secretes anti-dinitolmide monoclonal antibodies has been deposited in Comprehensive Microbiology Center of China Microbial Culture Collection Management Committee (CGMCC), addressed in No. 1 Hospital No. 3 Institute of Microbiology of the Chinese Academy of Sciences, North Chenxi Road, Beijing Chaoyang District in Beijing. It is classified as a monoclonal cell strain. The deposit date is Nov. 28, 2019, and the deposit number is MCCC No. 19165. The monoclonal antibody secreted by the hybridoma cell strain DAS3H10 has a good affinity and high sensitivity to dinitolmide. Because of IC50 to dinitolmide up to 9.01 ng/mL, the monoclonal antibody could be used to prepare dinitolmide immunoassay kits and colloidal gold test strips, and can further provide a powerful means for detecting dinitolmide in animal-derived foods.

Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.

Abstract: Pseudomonas and a use thereof are provided. The preservation number of the Pseudomonas is CGMCC No. 22583. The growth temperature of the Pseudomonas is 37-45° C. Under a medium-high temperature oil reservoir condition, the Pseudomonas can be denatured from a non-viscosity fluid to a multifunctional oil-extraction bacterial solution having both surface activity and viscoelasticity, and also has the functions of expanding the swept volume and improving the oil washing efficiency. The recovery ratio is increased by more than 20% in a physical simulation experiment.

Abstract: The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.

Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.

Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.

Abstract: A pixel bank manufacturing method, a pixel bank structure, a pixel structure, and a display panel are provided. The method includes providing a base substrate, wherein a plurality of anode thin film layers are manufactured on the base substrate; coating a photoresist layer used for covering the plurality of anode thin film layers on the base substrate; performing a photolithography on the photoresist layer by an exposing patterning structure, and baking to cure a remained photoresist layer after the photolithography to form a first bank layer; the exposing patterning structure is a structure that full via holes, first half via holes, a plurality of blind via holes, and second half via holes are arranged repeatedly; forming a second bank layer on the first bank layer; the second bank layer is a black bank layer.

Abstract: The present invention discloses an erasure code calculation method, including the following steps: S1) splitting original data, and building an original encoding matrix M; S2) acquiring a transverse exclusive OR encoding matrix M1; S3) acquiring a longitudinal exclusive OR encoding matrix M2; S4) acquiring an exclusive OR encoding matrix M3 according to the transverse exclusive OR encoding matrix M1 and the longitudinal exclusive OR encoding matrix M2; S5) transforming a data position of the transverse exclusive OR encoding matrix M1 to acquire a storage matrix M4; S6) judging whether storage nodes at which the last column of data of the storage matrix M4 is stored are damaged; S7) restoring the lost data according to a position 1 of the damaged node; and S8) restoring the lost data according to a position 2 of the damaged node. In the present invention, the operation is rapid, and calculation efficiency is high.

Abstract: A hybridoma cell strain secreting anti-dinitolmide monoclonal antibodies deposited at China General Microbiological Culture Collection Center (CGMCC) at No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China on Nov. 28, 2019, with deposit number of CGMCC No. 19165. It is classified as a monoclonal cell strain. The monoclonal antibody secreted by the hybridoma cell strain DAS3H10 has a good affinity and high sensitivity to dinitolmide. Because of IC50 to dinitolmide up to 9.01 ng/mL, the monoclonal antibody could be used to prepare dinitolmide immunoassay kit and colloidal gold test strip and provides a powerful detection method and means for the detection of dinitolmide in animal-derived foods.

Abstract: A pixel bank manufacturing method, a pixel bank structure, a pixel structure, and a display panel are provided. The method includes providing a base substrate, wherein a plurality of anode thin film layers are manufactured on the base substrate; coating a photoresist layer used for covering the plurality of anode thin film layers on the base substrate; performing a photolithography on the photoresist layer by an exposing patterning structure, and baking to cure a remained photoresist layer after the photolithography to form a first bank layer; the exposing patterning structure is a structure that full via holes, first half via holes, a plurality of blind via holes, and second half via holes are arranged repeatedly; forming a second bank layer on the first bank layer; the second bank layer is a black bank layer.

Abstract: The present invention discloses an erasure code calculation method, including the following steps: S1) splitting original data, and building an original encoding matrix M; S2) acquiring a transverse exclusive OR encoding matrix M1; S3) acquiring a longitudinal exclusive OR encoding matrix M2; S4) acquiring an exclusive OR encoding matrix M3 according to the transverse exclusive OR encoding matrix M1 and the longitudinal exclusive OR encoding matrix M2; S5) transforming a data position of the transverse exclusive OR encoding matrix M1 to acquire a storage matrix M4; S6) judging whether storage nodes at which the last column of data of the storage matrix M4 is stored are damaged; S7) restoring the lost data according to a position 1 of the damaged node; and S8) restoring the lost data according to a position 2 of the damaged node. In the present invention, the operation is rapid, and calculation efficiency is high.

Abstract: A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.