Patents by Inventor Xiaoling Wu

Xiaoling Wu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12286488
    Abstract: Disclosed is a hybridoma cell strain that secretes anti-dinitolmide monoclonal antibodies applicable to the field of food safety immunoassay methods. The hybridoma cell strain DAS3H10 that secretes anti-dinitolmide monoclonal antibodies has been deposited in Comprehensive Microbiology Center of China Microbial Culture Collection Management Committee (CGMCC), addressed in No. 1 Hospital No. 3 Institute of Microbiology of the Chinese Academy of Sciences, North Chenxi Road, Beijing Chaoyang District in Beijing. It is classified as a monoclonal cell strain. The deposit date is Nov. 28, 2019, and the deposit number is MCCC No. 19165. The monoclonal antibody secreted by the hybridoma cell strain DAS3H10 has a good affinity and high sensitivity to dinitolmide. Because of IC50 to dinitolmide up to 9.01 ng/mL, the monoclonal antibody could be used to prepare dinitolmide immunoassay kits and colloidal gold test strips, and can further provide a powerful means for detecting dinitolmide in animal-derived foods.
    Type: Grant
    Filed: July 6, 2021
    Date of Patent: April 29, 2025
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Chuanlai Xu, Jie Liu, Hua Kuang, Liguang Xu, Maozhong Sun, Liqiang Liu, Xiaoling Wu, Changlong Hao, Shanshan Song, Yongming Hu, Qiankun Zheng
  • Patent number: 12247085
    Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.
    Type: Grant
    Filed: December 27, 2022
    Date of Patent: March 11, 2025
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Chuanlai Xu, Hua Kuang, Wei Jiang, Liguang Xu, Wei Ma, Liqiang Liu, Xiaoling Wu, Shanshan Song, Yongming Hu
  • Publication number: 20240392235
    Abstract: Pseudomonas and a use thereof are provided. The preservation number of the Pseudomonas is CGMCC No. 22583. The growth temperature of the Pseudomonas is 37-45° C. Under a medium-high temperature oil reservoir condition, the Pseudomonas can be denatured from a non-viscosity fluid to a multifunctional oil-extraction bacterial solution having both surface activity and viscoelasticity, and also has the functions of expanding the swept volume and improving the oil washing efficiency. The recovery ratio is increased by more than 20% in a physical simulation experiment.
    Type: Application
    Filed: September 16, 2022
    Publication date: November 28, 2024
    Inventors: Weidong WANG, Jing HU, Yanbin CAO, Qin QIAN, Lei ZHANG, Liaoyuan GUO, Gongze CAO, Gangzheng SUN, Junzhang LIN, Yongting SONG, Shenghui YUE, Changzhong YUAN, Xiaoling WU, Mingshan DING, Guangjun GAO, Jing WANG, Tao LIU, Yun FENG, Caifeng LI, Xin SONG, Zihui CHEN
  • Patent number: 11753615
    Abstract: The present disclosure belongs to the field of bioengineering, and relates to breeding of industrial microorganisms, in particular to a genetically engineered strain of Saccharomyces cerevisiae, method for constructing the same, and its use for brewing, the genetically engineered strain of Saccharomyces cerevisiae heterogeneously overexpresses an acetaldehyde dehydrogenase gene ALD6, an acetyl-CoA synthase gene ACS1 and an alcohol acyltransferase gene AeAT9. The Saccharomyces cerevisiae strain with high yield of ethyl acetate and low yield of higher alcohols provided by the present disclosure not only maintains excellent ethanol fermentation characteristics, but also reducing the production of higher alcohols which adversely affect the comfort after drinking, which is of great significance for a well-maintained and strengthened flavor characteristics of Chinese Baijiu, an improved and stabilized quality thereof, and even a reform in the fermentation process thereof.
    Type: Grant
    Filed: August 25, 2021
    Date of Patent: September 12, 2023
    Assignee: Tianjin University of Science and Technology
    Inventors: Yefu Chen, Wenqi Shi, Guo Zhang, Ruirui Li, Yanfang Chen, Huan Wang, Chunhong Sun, Dongguang Xiao, Xiaole Wu, Xuewu Guo
  • Patent number: 11746353
    Abstract: A Saccharomyces cerevisiae strain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced.
    Type: Grant
    Filed: December 25, 2020
    Date of Patent: September 5, 2023
    Assignee: Tianjin University of Science and Technology
    Inventors: Yefu Chen, Yanrui Ma, Yongjing Du, Sen Jiang, Jinying Ren, Guo Zhang, Xinyue Kang, Peng Zheng, Xiaole Wu, Dongguang Xiao, Xuewu Guo
  • Publication number: 20230167196
    Abstract: The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.
    Type: Application
    Filed: November 9, 2021
    Publication date: June 1, 2023
    Inventors: Chuanlai XU, Jingjing YAO, Hua KUANG, Liguang XU, Maozhong SUN, Xiaoling WU, Liqiang LIU, Wei MA, Jianping ZHU, Changlong HAO, Shanshan SONG, Yongming HU, Aihong WU, Lingling GUO, Xinxin XU
  • Publication number: 20230133974
    Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.
    Type: Application
    Filed: December 27, 2022
    Publication date: May 4, 2023
    Inventors: Chuanlai XU, Hua KUANG, Wei JIANG, Liguang XU, Wei MA, Liqiang LIU, Xiaoling WU, Shanshan SONG, Yongming HU
  • Patent number: 11603414
    Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: March 14, 2023
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Chuanlai Xu, Hua Kuang, Wei Jiang, Liguang Xu, Wei Ma, Liqiang Liu, Xiaoling Wu, Shanshan Song, Yongming Hu
  • Publication number: 20220389371
    Abstract: The present disclosure belongs to the field of bioengineering, and relates to breeding of industrial microorganisms, in particular to a genetically engineered strain of Saccharomyces cerevisiae, method for constructing the same, and its use for brewing, the genetically engineered strain of Saccharomyces cerevisiae heterogeneously overexpresses an acetaldehyde dehydrogenase gene ALD6, an acetyl-CoA synthase gene ACS1 and an alcohol acyltransferase gene AeAT9. The Saccharomyces cerevisiae strain with high yield of ethyl acetate and low yield of higher alcohols provided by the present disclosure not only maintains excellent ethanol fermentation characteristics, but also reducing the production of higher alcohols which adversely affect the comfort after drinking, which is of great significance for a well-maintained and strengthened flavor characteristics of Chinese Baijiu, an improved and stabilized quality thereof, and even a reform in the fermentation process thereof.
    Type: Application
    Filed: August 25, 2021
    Publication date: December 8, 2022
    Inventors: Yefu Chen, Wenqi Shi, Guo Zhang, Ruirui Li, Yanfang Chen, Huan Wang, Chunhong Sun, Dongguang Xiao, Xiaole Wu, Xuewu Guo
  • Patent number: 11404680
    Abstract: A pixel bank manufacturing method, a pixel bank structure, a pixel structure, and a display panel are provided. The method includes providing a base substrate, wherein a plurality of anode thin film layers are manufactured on the base substrate; coating a photoresist layer used for covering the plurality of anode thin film layers on the base substrate; performing a photolithography on the photoresist layer by an exposing patterning structure, and baking to cure a remained photoresist layer after the photolithography to form a first bank layer; the exposing patterning structure is a structure that full via holes, first half via holes, a plurality of blind via holes, and second half via holes are arranged repeatedly; forming a second bank layer on the first bank layer; the second bank layer is a black bank layer.
    Type: Grant
    Filed: April 22, 2020
    Date of Patent: August 2, 2022
    Assignee: Shenzhen China Star Optoelectronics Semiconductor Display Technology Co., Ltd.
    Inventor: Xiaoling Wu
  • Publication number: 20220168753
    Abstract: An electrostatic dust removal apparatus and an electrode unit thereof are disclosed. The electrostatic dust removal apparatus includes a frame, and a plurality of electrode units. The plurality of electrode units include at least one high-potential unit and at least one low-potential unit. The plurality of electrode units are provided on the frame and are spaced apart from each other. The at least one high-potential unit and the at least one low-potential unit are arranged alternately, and an air channel for removing dust is formed between the electrode units adjacent to each other. Each of the plurality of electrode units includes a conducting part and an insulating part, the conducting part is a conductive plastic part and includes an electrical field generator and a conducting end, and the insulating part covers at least a portion of the electrical field generator.
    Type: Application
    Filed: February 18, 2022
    Publication date: June 2, 2022
    Inventors: Jih Teck TIONG, XiaoLe WU
  • Patent number: 11303302
    Abstract: The present invention discloses an erasure code calculation method, including the following steps: S1) splitting original data, and building an original encoding matrix M; S2) acquiring a transverse exclusive OR encoding matrix M1; S3) acquiring a longitudinal exclusive OR encoding matrix M2; S4) acquiring an exclusive OR encoding matrix M3 according to the transverse exclusive OR encoding matrix M1 and the longitudinal exclusive OR encoding matrix M2; S5) transforming a data position of the transverse exclusive OR encoding matrix M1 to acquire a storage matrix M4; S6) judging whether storage nodes at which the last column of data of the storage matrix M4 is stored are damaged; S7) restoring the lost data according to a position 1 of the damaged node; and S8) restoring the lost data according to a position 2 of the damaged node. In the present invention, the operation is rapid, and calculation efficiency is high.
    Type: Grant
    Filed: March 11, 2021
    Date of Patent: April 12, 2022
    Assignee: BEIJING iChinaE SCIENCE & TECHNOLOGY CO., LTD.
    Inventors: Siqi Wu, Xiaoling Wu
  • Publication number: 20220010029
    Abstract: A hybridoma cell strain secreting anti-dinitolmide monoclonal antibodies deposited at China General Microbiological Culture Collection Center (CGMCC) at No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China on Nov. 28, 2019, with deposit number of CGMCC No. 19165. It is classified as a monoclonal cell strain. The monoclonal antibody secreted by the hybridoma cell strain DAS3H10 has a good affinity and high sensitivity to dinitolmide. Because of IC50 to dinitolmide up to 9.01 ng/mL, the monoclonal antibody could be used to prepare dinitolmide immunoassay kit and colloidal gold test strip and provides a powerful detection method and means for the detection of dinitolmide in animal-derived foods.
    Type: Application
    Filed: July 6, 2021
    Publication date: January 13, 2022
    Inventors: Chuanlai XU, JIE LIU, HUA KUANG, Liguang XU, Maozhong SUN, Liqiang LIU, Xiaoling WU, Changlong HAO, Shanshan SONG, Yongming HU, Qiankun ZHENG
  • Patent number: D943136
    Type: Grant
    Filed: February 18, 2019
    Date of Patent: February 8, 2022
    Inventor: Xiaoling Wu
  • Patent number: D970769
    Type: Grant
    Filed: July 16, 2019
    Date of Patent: November 22, 2022
    Inventor: Xiaoling Wu
  • Patent number: D975001
    Type: Grant
    Filed: March 16, 2021
    Date of Patent: January 10, 2023
    Inventor: Xiaoling Wu
  • Patent number: D984705
    Type: Grant
    Filed: February 21, 2022
    Date of Patent: April 25, 2023
    Inventor: Xiaoling Wu
  • Patent number: D984714
    Type: Grant
    Filed: November 15, 2022
    Date of Patent: April 25, 2023
    Inventor: Xiaoling Wu
  • Patent number: D1006287
    Type: Grant
    Filed: November 15, 2022
    Date of Patent: November 28, 2023
    Inventor: Xiaoling Wu
  • Patent number: D1063151
    Type: Grant
    Filed: March 26, 2024
    Date of Patent: February 18, 2025
    Inventor: Xiaoling Wu