Abstract: The present disclosure relates to a biochemical detection instrument. The technical solution is an automatic reactive oxygen species content detection system suitable for a cell microenvironment that includes: a sample transmission reaction system and a detection system which are communicated in sequence through a light avoiding pipeline. A washing system is in communication with the sample transmission reaction system through a water pipeline, and a purge system is in communication with the sample transmission reaction system through a gas pipeline. The sample transmission reaction system further includes a sample injector and a DCFH supply bin which are communicated with a reaction bin through light avoiding pipelines after being connected in parallel. Sample injection valves are respectively configured between the sample injection valve and the reaction bin and between the DCFH supply bin and the reaction bin.

Abstract: The present disclosure relates to a biochemical detection instrument. The technical solution is an automatic reactive oxygen species content detection system suitable for a cell microenvironment that includes: a sample transmission reaction system and a detection system which are communicated in sequence through a light avoiding pipeline. A washing system is in communication with the sample transmission reaction system through a water pipeline, and a purge system is in communication with the sample transmission reaction system through a gas pipeline. The sample transmission reaction system further includes a sample injector and a DCFH supply bin which are communicated with a reaction bin through light avoiding pipelines after being connected in parallel. Sample injection valves are respectively configured between the sample injection valve and the reaction bin and between the DCFH supply bin and the reaction bin.

Abstract: An optical system includes a spectrograph having a concave diffraction grating and a detector. An aperture is selectively positioned by an associated actuator or positioning mechanism either into, or out of, an optical path of the input light beam downstream of a sample and prior to entering the spectrograph. A slit plate having a plurality of different size entrance slits is positioned downstream of the aperture and movable by an associated actuator or positioning mechanism to position one of the plurality of entrance slits in the optical path of the input light beam. A controller coupled to the detector and the actuators is configured to control the actuators to selectively position the aperture and the slit plate to provide a selectable resolution of the spectrograph. The aperture setting and slit plate setting may be determined from a lookup table in response to a request for finer or coarser spectral resolution.

Abstract: An optical system includes a spectrograph having a concave diffraction grating and a detector. An aperture is selectively positioned by an associated actuator or positioning mechanism either into, or out of, an optical path of the input light beam downstream of a sample and prior to entering the spectrograph. A slit plate having a plurality of different size entrance slits is positioned downstream of the aperture and movable by an associated actuator or positioning mechanism to position one of the plurality of entrance slits in the optical path of the input light beam. A controller coupled to the detector and the actuators is configured to control the actuators to selectively position the aperture and the slit plate to provide a selectable resolution of the spectrograph. The aperture setting and slit plate setting may be determined from a lookup table in response to a request for finer or coarser spectral resolution.

Abstract: A system or method for analyzing a sample include an input light source, a double subtractive monochromator positioned to receive light from the input light source and to sequentially illuminate the sample with each of a plurality of wavelengths, a multi-channel fluorescence detector positioned to receive and substantially simultaneously detect multiple wavelengths of light emitted by the sample for each of the plurality of excitation wavelengths, an absorption detector positioned to receive and detect light passing through the sample, and a computer in communication with the monochromator, the fluorescence detector, and the absorption detector, the computer controlling the monochromator to sequentially illuminate the sample with each of the plurality of wavelengths while measuring absorption and fluorescence of the sample based on signals received from the fluorescence and absorption detectors.

Abstract: A system or method for analyzing a sample include an input light source, a double subtractive monochromator positioned to receive light from the input light source and to sequentially illuminate the sample with each of a plurality of wavelengths, a multi-channel fluorescence detector positioned to receive and substantially simultaneously detect multiple wavelengths of light emitted by the sample for each of the plurality of excitation wavelengths, an absorption detector positioned to receive and detect light passing through the sample, and a computer in communication with the monochromator, the fluorescence detector, and the absorption detector, the computer controlling the monochromator to sequentially illuminate the sample with each of the plurality of wavelengths while measuring absorption and fluorescence of the sample based on signals received from the fluorescence and absorption detectors

Abstract: A forensic light source which comprises a flexible liquid light guide receiving light from a light source and transmitting it to a selected interference filter which tilts with respect to the light source is disclosed. The filter is mounted for rotation with respect to the output of the light guide. The light exiting the filter is passed through a mixing member made of a randomized fiber optic bundle, that is positioned to receive the output of the filter. The mixing member defines multiple paths for light between the input face and the output face which are configured to disperse light from one mixing member input face region to a plurality of mixing member output face regions.