Abstract: Disclosed is a method for boosting genetic transformation efficiency of wheat using an Agrobacterium-mediated method, which belongs to the field of plant genetic engineering technology. A TaHRF2 gene and an encoded protein thereof used in the present disclosure can promote introduction of a nucleic acid molecule into a plant of interest and boost genetic transformation efficiency of the introduction of the nucleic acid molecule into the plant of interest. The plant of interest includes, but is not limited to, monocotyledonous plants such as wheat. By introducing a TaHRF2 gene high-expression vector into an Agrobacterium strain, and infecting a wheat explant using an Agrobacterium-mediated method, genetic transformation capacities of backbone parents such as Beijing 8 and Lumai 1 are enhanced, providing important information and fundamental technical support for the establishment of an efficient genetic transformation system for major wheat cultivars with difficulty in regeneration.

Abstract: Disclosed is a gene for boosting genetic transformation efficiency of wheat and use thereof, which belongs to the field of plant genetic engineering technology. The use of a TaHRF1 gene and an encoded protein thereof can promote integration of an exogenous nucleic acid molecule into a genome of a plant of interest, and boost efficiency of the integration of the nucleic acid molecule, and the transformed plant grows and develops normally. The plant of interest includes, but is not limited to, monocotyledonous plants such as wheat. The present disclosure provides a new and important genetic resource for overcoming the genotype dependence in genetic transformation of wheat.

Abstract: Disclosed is a method for boosting genetic transformation efficiency of wheat using an Agrobacterium-mediated method, which belongs to the field of plant genetic engineering technology. A TaHRF2 gene and an encoded protein thereof used in the present disclosure can promote introduction of a nucleic acid molecule into a plant of interest and boost genetic transformation efficiency of the introduction of the nucleic acid molecule into the plant of interest. The plant of interest includes, but is not limited to, monocotyledonous plants such as wheat. By introducing a TaHRF2 gene high-expression vector into an Agrobacterium strain, and infecting a wheat explant using an Agrobacterium-mediated method, genetic transformation capacities of backbone parents such as Beijing 8 and Lumai 1 are enhanced, providing important information and fundamental technical support for the establishment of an efficient genetic transformation system for major wheat cultivars with difficulty in regeneration.

Abstract: Disclosed is a gene for boosting genetic transformation efficiency of wheat and use thereof, which belongs to the field of plant genetic engineering technology. The use of a TaHRF1 gene and an encoded protein thereof can promote integration of an exogenous nucleic acid molecule into a genome of a plant of interest, and boost efficiency of the integration of the nucleic acid molecule, and the transformed plant grows and develops normally. The plant of interest includes, but is not limited to, monocotyledonous plants such as wheat. The present disclosure provides a new and important genetic resource for overcoming the genotype dependence in genetic transformation of wheat.