Abstract: The invention relates to cell biology and regenerative medicine, specifically to a culture method and application for porcine limbal epithelial stem cells. The method involves pretreatment of cells, coating of culture dishes, centrifugation, culturing, passaging, and identification by immunofluorescence staining. The cultured cells are applicable in repairing corneal diseases, including limbal stem cell deficiency. During repair, the cells can be combined with biological materials to form a stem cell engineering carrier for implantation at the injury site. The separation and culture method is simple, yields a large number of cells, and is suitable for use with porcine eyeballs. Limbal epithelial stem cells cultured in vitro form distinct cell clusters, referred to as stem cell islands. The obtained cells exhibit phenotypes including BMI1+, ?Np63?+, CK14+, and ABCG2+.

Abstract: The invention relates to bioinformatics technology and specifically to an analysis method of a spatial transcriptome-based 4D-printed stem cell scaffold for enhancing diabetic skin injury healing. Spatial transcriptome sequencing is performed on tissues from both the PBS group and the combined treatment group. Cell clustering and annotation identify main cell types, followed by visualization of their spatial distributions in both groups. The proportions of various cell types are statistically quantified and plotted. Cell subsets exhibiting significant differential gene expression are enriched and verified. Using a 4D-printed stem cell scaffold combined with a piRNA inhibitor and stem cell therapy, the method reveals spatial distribution and dynamic cellular changes during wound healing. It identifies precise cellular locations and gene expression patterns within tissues, providing critical insights into the biological processes by which biomaterials promote diabetic injury repair.

Abstract: The present invention relates to cell biology and regenerative medicine, specifically to a method for isolating and culturing porcine endometrial mesenchymal stem cells (PEMSCs) and their applications. PEMSCs are sourced from uterine tissue of pigs, providing a streamlined process yielding a substantial quantity of cells. Cultured PEMSCs exhibit CD34?, CD11b?, CD44+, and CD105+ phenotypic markers and can differentiate into osteocytes, adipocytes, and chondrocytes in vitro. The method employs type II collagenase to degrade extracellular matrix collagen and DNase I to break down DNA, reducing cell aggregation. This combination ensures efficient tissue digestion and high-quality single-cell suspensions. Applications include tissue repair for intrauterine adhesions, skin injuries, and vascular damage. The repair involves combining PEMSCs with biomaterials and implanting them into lesion sites.

Abstract: An application of Bone Marrow Mesenchyml Stem Cell Exosomes (BMSC-Exos) in treating Parkinson's disease (PD) is provided, wherein the BMSC-Exos are generated by stimulating BMSCs with a culture solution and extracted from the culture solution after passage; and the culture solution of the BMSCs is an ?-MEM culture solution containing FBS and PS. The BMSC-Exos can greatly improve a motor function of a model mouse with PD, protect dopaminergic neurons of the model mouse with PD, improve an olfactory function of the model mouse with PD, and also inhibit the activation of olfactory astrocytes of the model mouse with PD.

Abstract: The present invention belongs to the field of biomedical technology, and specifically relates to a new application for piR-hsa-164586 and MYH9, based on the interaction between piR-hsa-164586 and MYH9: piR-hsa-164586 regulates the expression of MYH9 and promotes the metastasis of NSCLC, piR-hsa-164586 and MYH9 can be used as a therapeutic target for NSCLC, and have become a potential therapeutic drug, wherein the MYH9 is obtained by RNA pulldown assay and verified by protein mass spectrometry, molecular docking and RIP assays, the piR-hsa-164586 can positively regulate the expression of MYH9, and verified by performed qRT-PCR, western blotting and tissue immunofluorescence staining assays on the piR-hsa-164586 NC group, piR-hsa-164586 KD group, and piR-hsa-164586 OE group. The interaction between piR-hsa-164586 and MYH9 can up-regulate the expression of MYH9 in NSCLC cell lines, the verification experiment process was confirmed by Transwell experiment after knockdown of piR-hsa-164586 and MYH9 respectively.

Abstract: Provided are the compositions (e.g., pharmaceutical compositions) of coagulation factor X activator (FX activator, e.g., RVV-X) comprising sucrose, histidine, polysorbate 20, and mannitol, and uses of FX activators (e.g., RVV-X) or compositions (e.g., pharmaceutical compositions) thereof for treating a bleeding disorder (e.g., hemophilia) in an individual (e.g., human).

Abstract: A traditional Chinese medicine compound preparation for treating Parkinson's disease and its preparation method and application are disclosed. The traditional Chinese medicine compound preparation comprises traditional Chinese medicine formula components and the traditional Chinese medicine formula components comprise the following raw material or raw material extracts by mass: 6-15 parts of uncaria rhynchophylla, 3-9 parts of gastrodia elata, 6-12 parts of Gentiana macrophylla, 10-20 parts of raw Astragalus membranaceus, 10-20 parts of prepared rehmannia roots, 10-20 parts of achyranthes roots, 10-20 parts of wolfberry fruits, 10-20 parts of angelica sinensis, 10-20 parts of fried atractylodes macrocephala, 6-15 parts of fried spina date seeds, 3-12 parts of walnut kernels, 3-9 parts of safflower, and 20-30 parts of zaocys dhumnade.

Abstract: A process method for preparing magnesium oxide from dolomite includes the following steps: S1, pretreating dolomite to obtain the calcined dolomite; S2, hydrating the calcined dolomite according to the solid-liquid ratio, controlling the hydration temperature and the hydration time, filtering, washing and drying after hydration to obtain the hydration product; S3, configuring a complex solution, using the sodium hydroxide solution to adjust the pH value to 8-10; S4, performing the complexation reaction by mixing the hydration product in S2 with the complex solution in S3, and controlling the complexation temperature and the complexation time, obtaining the magnesium oxide by filtration, washing and drying after the reaction is completed. The invention uses dolomite as raw material, and the amount of magnesium hydroxide produced by magnesium oxide at a specific hydration temperature and hydration time can be ignored.

Abstract: An application of Bone Marrow Mesenchyml Stem Cell Exosomes (BMSC-Exos) in treating Parkinson's disease (PD) is provided, wherein the BMSC-Exos are generated by stimulating BMSCs with a culture solution and extracted from the culture solution after passage; and the culture solution of the BMSCs is an ?-MEM culture solution containing FBS and PS. The BMSC-Exos can greatly improve a motor function of a model mouse with PD, protect dopaminergic neurons of the model mouse with PD, improve an olfactory function of the model mouse with PD, and also inhibit the activation of olfactory astrocytes of the model mouse with PD.

Abstract: A low-concentration gas differential combustion device includes a low-concentration gas super-cooling dehydration and demisting device, a gas pretreatment device, a burner, a long-term burning open fire device, a high-energy self-heat dispersion rapid ignition device, a combustion chamber, and a waste-heat utilization device. With the low-concentration gas differential combustion device, the problems of gas escaping, forced direct emission, low heat extraction efficiency, concentration over-limit explosions, increase in equipment volume and increase in investment caused by the reversal process in the existing ventilation air oxidation technology, and the problems of narrow gas adaptation concentration range, small adaptive concentration and pressure change amplitude, poor combustion temperature adjustability, high NOx content, high shutdown rate, and low gas utilization rate in the low-concentration gas internal combustion engine power generation technology are solved.

Abstract: An optical fiber output light source device includes an optical path that is obtained by joining a polarization maintaining amplifying optical fiber to a polarization maintaining dispersion compensating fiber, a single-polarization reflective polarizing beam splitter that is joined to one end of the optical path, a 90° polarization-rotating reflector that is joined to the other end of the optical path, a multiplexer that is inserted in the optical path, and a pumping light source that is joined to the multiplexer. An axis roll connection portion is provided in which a polarization axis of light returned from the single-polarization reflective polarizing beam splitter to the optical path is connected to a polarization maintaining axis of the optical path with a twisting angle therebetween at a predetermined angle.

Abstract: A pushing device and method of a media resource, an electronic device, and a storage device are described. The method for pushing the media resource includes determining an objective sliding path according to acquired path information; detecting a gesture operation on an interface for displaying a to-be-pushed media resource to determine a sliding path of the gesture operation; determining whether the sliding path of the gesture operation matches the objective sliding path when a sliding path similarity is greater than or equal to a first threshold, and that the sliding path of the gesture operation does not match the objective sliding path when the sliding path similarity is less than the first threshold; and generating a pushing instruction for pushing the to-be pushed media resource in response to the sliding path matching the objective sliding path.

Abstract: This application provides an image processing apparatus and method. The apparatus includes: a first processing unit, configured to obtain a first stitched image based on a first image and a second image; and a sending unit, configured to send the first stitched image, where a quantity of row pixels of the first image is M, and a quantity of column pixels is N; a quantity of row pixels of the second image is P, a quantity of column pixels is Q, and Q?M; and a quantity of row pixels of the first stitched image is N+P, and a quantity of column pixels is Q. The first stitched image includes the second image and a third image. The third image is an image obtained through first preprocessing of the first image. The first preprocessing includes rotating the first image, or the first preprocessing includes rotating and padding the first image.

Abstract: A Fructus Forsythiae and Radix Astragali compound preparation, and a preparation method therefor and a use thereof is provided. Traditional Chinese medicine formulation components consist of the following raw materials or raw material extracts in parts by mass: 9-11 parts of Flos Lonicerae, 9-11 parts of Fructus Forsythiae, 9-11 parts of Radix Scutellariae, 9-11 parts of Herba Artemisiae Annuae, 9-11 parts of Radix Astragali, 9-11 parts of stir-fried Rhizoma Atractylodis Macrocephalae, 9-11 parts of Herba Pogostemonis, 5-7 parts of Radix Saposhnikoviae, 9-11 parts of Radix Ophiopogonis, and 5-7 parts of Radix Glycyrrhizae. The components can be made into an oral liquid, a granule, a dissolved medicine, or a tablet. Further disclosed is a use of the Fructus Forsythiae and Radix Astragali compound preparation in preparation of medicines for preventing and/or treating a viral influenza disease.

Abstract: The present disclosure relates to a method and an apparatus for controlling a screen display content of a display terminal, an electronic device, and a computer-readable storage medium, which belongs to the field of image display technology. The method includes: acquiring angular acceleration data collected by a motion sensor in a terminal device, and determining a movement trajectory of the terminal device according to the angular acceleration data; determining an operation instruction corresponding to the movement trajectory of the terminal device according to a preset mapping relationship between the movement trajectory and the operation instruction; and sending the operation instruction to control a screen of the display terminal to switch the display content.