Patents by Inventor Xiaonan Xu

Xiaonan Xu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12357989
    Abstract: The present invention provides methods and devices for detecting and quantifying multiple biomolecules at single-molecule level using an integrated droplet microfluidic system. In one embodiment, the present invention provides real-time and digital measurement of multiple biomolecules in a sample, thereby quantifying multiple biomolecules in an absolute and simultaneous manner. In one embodiment, the present invention provides a diagnostic method for a disease, comprising real-time and digital measurement of multiple biomolecules in a sample using the method or device described herein.
    Type: Grant
    Filed: August 26, 2019
    Date of Patent: July 15, 2025
    Assignee: Zhejiang Dapu Biotechnology Co., Ltd.
    Inventors: Xiaonan Xu, Ruyuan Song, Shuhuai Yao, Kwok Fai Joseph Chow
  • Publication number: 20240360480
    Abstract: A marine-derived nanoselenium-producing strain and a method for preparing nanoselenium by using the strain are provided in the present disclosure, belonging to the field of nano biomaterials. According to the present disclosure, a Bacillus strain capable of preparing nanoselenium is screened from sediments taken from the Arctic Ocean, named Bacillus sp. Q72, which has been deposit in the China General Microbiological Culture Collection Center, and the deposit number is CGMCC No.26355. The extracellular fluid of this strain is used to directly reduce high concentrations of sodium selenite to obtain selenium nanoparticles.
    Type: Application
    Filed: July 12, 2024
    Publication date: October 31, 2024
    Applicant: Dalian Minzu University
    Inventors: Chunshan Quan, Liying Zhang, Liming Jin, Haodi Ma, Xiaonan Xu, Yan Xu, Mingjie Ren
  • Publication number: 20230285971
    Abstract: The present invention relates to the field of droplet microfluidics, particularly relates to a droplet sorting chip. The droplet sorting chip disclosed herein is provided with a cavity area, which can accommodate impurities such as fibers entering the droplet sorting chip, can effectively prevent clogging and collision and fusion of droplets caused by impurities, and ensure smooth droplet sorting.
    Type: Application
    Filed: April 30, 2022
    Publication date: September 14, 2023
    Inventors: Hongbo ZHOU, Ruyuan SONG, Xiaonan XU
  • Publication number: 20230201834
    Abstract: The present invention provides a droplet generator. The droplet generator includes a large-corner continuous U-shaped flow channel on the encoded microsphere flow channel. Controlling the turning radius of the continuous U-shaped flow channel prevents the blockage resulted from accumulation of microfibers in the encoded microsphere suspension at the turn of the continuous U-shaped flow channel. A buffer tank is arranged at the encoded microsphere flow channel, which is used for the pre-arrangement of the encoded microspheres, to increase the controllability of the flow rate of the encoded microspheres. The buffer tank is provided in the oil-phase flow channel to prevent the oil phase from infiltrating into the aqueous phase due to excessive flow rate, and increase the controllability of the oil phase flow rate. The droplet generator has simple structure, low cost, high-throughput, and high-stability, and may be used to prepare single-cell single-encoded microsphere droplets.
    Type: Application
    Filed: April 7, 2022
    Publication date: June 29, 2023
    Inventors: Hongbo ZHOU, Ruyuan SONG, Xiaonan XU
  • Publication number: 20220074929
    Abstract: Methods of quantification, isolation, and characterization of exosomes are provided. Exosomes can be quantified by contacting a sample with a capture bead comprising a bead and a first binding agent, and a second binding agent. The first binding agent binds to a first biomolecule in the exosomes to produce a first complex and the second binding agent binds to a second biomolecule in the exosomes of the first complex to produce a second complex. The first complexes and the second complexes are quantified based on a detectable signal conjugated to the second binding agent. A microwell or a droplet generation is utilized to quantify the first complexes and the second complexes. Quantifying the exosomes is used to diagnose a cancer in a subject. In such methods, the first and the second binding agents bind to cancer biomarkers present in the exosomes.
    Type: Application
    Filed: October 11, 2018
    Publication date: March 10, 2022
    Inventors: Shuhuai YAO, Lei ZHENG, Xiaonan XU, Chunchen LIU, Yu HU
  • Publication number: 20220041967
    Abstract: The present invention relates to methods and devices for monitoring events occurred in a single cell or examining cell characteristics in a single cell in a massive parallel and real-time manner. In one embodiment, the present invention provides a single-cell culturing system for culturing and monitoring a large number of cells independently at single-cell level. In one embodiment, the present invention provides methods and devices for studying or monitoring single-cell response to an external stimulus in a massive parallel and real-time manner. In one embodiment, the present invention provides methods and devices for studying or monitoring drug response at single-cell level in a massive parallel and real-time manner.
    Type: Application
    Filed: September 18, 2019
    Publication date: February 10, 2022
    Inventors: Ruyuan SONG, Xiaonan XU, Shuhuai YAO, Kwok Fai Joseph CHOW
  • Publication number: 20210370303
    Abstract: The present invention provides a microfluidic circuit for generating uniform droplets despite fluctuations in pressure, and manufacturing methods and uses thereof. Said circuit comprises microfluidic channels for carrying a continuous phase and a dispersed phase. In one embodiment, the ratio of the flow resistance of the dispersed phase to that of the continuous phase is equal to the ratio of the flow rate of the continuous phase to that of the dispersed phase. In one embodiment, the present microfluidic circuit comprises two features to achieve the desired ratio of flow resistance and flow rate of the dispersed phase and continuous phase: (a) using a single pressure source which applies identical pressure to the inlets of the upstream channels carrying the two phases, and (b) the flow resistance of the dispersed phase and continuous phase is much higher than the flow resistance of the downstream channel so that the flow resistance of the downstream channel become negligible.
    Type: Application
    Filed: October 19, 2019
    Publication date: December 2, 2021
    Inventors: Xiaonan XU, Ruyuan SONG, Shuhuai YAO, Kwok Fai Joseph CHOW
  • Publication number: 20210308678
    Abstract: The present invention provides methods and devices for detecting and quantifying multiple biomolecules at single-molecule level using an integrated droplet microfluidic system. In one embodiment, the present invention provides real-time and digital measurement of multiple biomolecules in a sample, thereby quantifying multiple biomolecules in an absolute and simultaneous manner. In one embodiment, the present invention provides a diagnostic method for a disease, comprising real-time and digital measurement of multiple biomolecules in a sample using the method or device described herein.
    Type: Application
    Filed: August 26, 2019
    Publication date: October 7, 2021
    Inventors: Xiaonan XU, Ruyuan SONG, Shuhuai YAO, Kwok Fai Joseph CHOW
  • Patent number: 10632479
    Abstract: A droplet generator apparatus and droplet generation method based on high aspect ratio induced droplet self-breakup are provided. The droplet generator apparatus includes a channel (1) and a nozzle (2) connected to the channel(1), and the aspect ratio of the channel (1) can be 3.0 or greater. The apparatus may further include a blocking rail (10) that is positioned in front of the nozzle (2), a supplying rail(9) that is positioned in front of the nozzle (2), and a supplying trench (8) formed in a space between the nozzle (2) and the supplying rail (9).
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: April 28, 2020
    Assignee: The Hong Kong University of Science and Technology
    Inventors: Shuhuai Yao, Hongbo Zhou, Xiaonan Xu, Jianjie Lu
  • Publication number: 20180085762
    Abstract: A droplet generator apparatus and droplet generation method based on high aspect ratio induced droplet self-breakup are provided. The droplet generator apparatus includes a channel (1) and a nozzle (2) connected to the channel(1), and the aspect ratio of the channel (1) can be 3.0 or greater. The apparatus may further include a blocking rail (10) that is positioned in front of the nozzle (2), a supplying rail(9) that is positioned in front of the nozzle (2), and a supplying trench (8) formed in a space between the nozzle (2) and the supplying rail (9).
    Type: Application
    Filed: May 20, 2016
    Publication date: March 29, 2018
    Inventors: Shuhuai Yao, Hongbo Zhou, Xiaonan Xu, Jianjie Lu