Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.

Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.

Abstract: A press hardening steel by a thin slab and having a tensile strength of 1900 MPa or more includes following components by weight: C: 0.31-0.40%, Si: 0.36-0.44%, Mn: 1.6-2.0%, P?0.006%, S?0.004%, Als: 0.015-0.060%, Cr: 0.36-0.49%, Ti: 0.036-0.045% or Nb: 0.036-0.045% or V: 0.036-0.045% or a mixture of any two or more of the above in any proportion, B: 0.004-0.005%, Mo: 0.26-0.35%, and N?0.005%. A method for producing the press hardening steel includes following steps: molten iron desulphurization; smelting and refining by an electric furnace or converter; continuous casting; descaling treatment before entering a soaking furnace; heating and soaking; high pressure water descaling before entering a rolling mill; hot rolling; cooling; coiling; austenitizing; die deforming and quenching.

Abstract: A press hardening steel by a thin slab and having a tensile strength of 1900 MPa or more includes following components by weight: C: 0.31-0.40%, Si: 0.36-0.44%, Mn: 1.6-2.0%, P?0.006%, S?0.004%, Als: 0.015-0.060%, Cr: 0.36-0.49%, Ti: 0.036-0.045% or Nb: 0.036-0.045% or V: 0.036-0.045% or a mixture of any two or more of the above in any proportion, B: 0.004-0.005%, Mo: 0.26-0.35%, and N?0.005%. A method for producing the press hardening steel includes following steps: molten iron desulphurization; smelting and refining by an electric furnace or converter; continuous casting; descaling treatment before entering a soaking furnace; heating and soaking; high pressure water descaling before entering a rolling mill; hot rolling; cooling; coiling; austenitizing; die deforming and quenching.

Abstract: A press hardening steel by a medium and thin slab casting and direct rolling has a tensile strength 1900 MPa or more. The press hardening steel includes following chemical components by weight percent: C: 0.31-0.40%, Si: 0.36-0.44%, Mn: 1.6-2.0%, P?0.006%, S?0.004%, Als: 0.015-0.060%, Cr: 0.36-0.49%, Ti: 0.036-0.045% or Nb: 0.036-0.045% or V: 0.036-0.045%, or a mixture of the above two or more in any ratio, B: 0.004-0.005%, Mo: 0.26-0.35%, Ni: 0.11-0.20%, and N?0.005%. A method for producing the press hardening steel includes following steps: desulphurizing molten iron; electric furnace or converter smelting and refining; continuous casting; descaling treatment before entering a soaking furnace; heating and soaking; high-pressure water descaling before entering a rolling mill; hot rolling; cooling; coiling; austenitizing; die deforming and quenching.

Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.

Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.