Abstract: The present disclosure relates to a communication method for a slave device in a master-slave communication structure, including: sending a boot report to the master device at power-up to report that the slave device is booting, wherein the boot report includes identity information for the slave device; receiving a heartbeat message from the master device; and if no heartbeat message is received from the master device for more than a predetermined time, the boot report is continuously sent to the master device at predetermined intervals until a feedback message of successful connection is received from the master device. Further, the present disclosure relates to a communication method for a communication system having a master-slave communication structure, a communication device for a slave device in a master-slave communication structure, and a communication system having a master-slave communication structure.

Abstract: Fluorescent adenosine derivatives that can be incorporated into the 5? end of transcribed RNA are disclosed. Adenosine derivatives have readily detectable cyanine dyes attached. Symmetric derivatives are disclosed that. The transcribed RNAs can be used in a variety of applications including nucleic acid detection and in the study of RNA structure and function. Methods of generating labeled RNAs from a biological sample also are disclosed.