Abstract: A method for site-specific conjugation of nucleic acid to a CRISPR family protein, and a conjugate thereof and the use thereof. The site-specific conjugation method comprises: mutating a CRISPR family protein site-specifically with an unnatural amino acid, and then conjugating a nucleic acid to the mutated CRISPR family protein, wherein the unnatural amino acid has orthogonal chemical reactivity. The CRISPR family protein site-specifically mutated and the conjugate thereof of the present application can be used to improve gene cutting, gene editing efficiency and base editing efficiency, etc.

Abstract: A facial invasive tissue treatment method includes the following procedures. Mark five to ten facelift suture path lines on each half face of a user in a predetermined pattern according to the facial condition of the user, wherein each facelift suture path line has an upper segment, a lower segment, and an insertion mark between the upper segment and the lower segment. After sterilization of the user's face, perform anesthesia at portions of the user's face where the needles inserting in and penetrating by injecting anesthetics at least at the insertion marks and the outlet marks of the facelift suture path lines marked on both half faces of the user. Thereafter, it is preferred to perform another sterilization to the user's face. Implant five to ten invasive sutures in the subcutaneous tissue layer of both half faces of the user along and underlying the facelift suture path lines marked thereon respectively.

Abstract: A facial invasive tissue treatment method includes the following procedures. Mark five to ten facelift suture path lines on each half face of a user in a predetermined pattern according to the facial condition of the user, wherein each facelift suture path line has an upper segment, a lower segment, and an insertion mark between the upper segment and the lower segment. After sterilization of the user's face, perform anesthesia at portions of the user's face where the needles inserting in and penetrating by injecting anesthetics at least at the insertion marks and the outlet marks of the facelift suture path lines marked on both half faces of the user. Thereafter, it is preferred to perform another sterilization to the user's face. Implant five to ten invasive sutures in the subcutaneous tissue layer of both half faces of the user along and underlying the facelift suture path lines marked thereon respectively.

Abstract: A facial invasive tissue treatment method includes the following procedures. Mark five to ten facelift suture path lines on each half face of a user in a predetermined pattern according to the facial condition of the user, wherein each facelift suture path line has an upper segment, a lower segment, and an insertion mark between the upper segment and the lower segment. After sterilization of the user's face, perform anesthesia at portions of the user's face where the needles inserting in and penetrating by injecting anesthetics at least at the insertion marks and the outlet marks of the facelift suture path lines marked on both half faces of the user. Thereafter, it is preferred to perform another sterilization to the user's face. Implant five to ten invasive sutures in the subcutaneous tissue layer of both half faces of the user along and underlying the facelift suture path lines marked thereon respectively.