Abstract: The present disclosure provides a method for analysis of the position distribution of lecithin fat acid, relating to the technical field of oil processing. The analysis method according to the present disclosure makes it possible to catalyze lecithin to complete alcoholysis in a short time with Novozym 435 or Lipozyme 435 in the present of excess anhydrous ethanol, thereby quickly and accurately analyzing the position distribution of lecithin fat acid. Novozym 435 or Lipozyme 435 exhibits a strong sn-1 position specificity and an extremely high reactivity to lecithin in the present of excess anhydrous ethanol, thereby greatly increasing the reaction rate of the alcoholysis to ensure quick and complete alcoholysis of lecithin, avoiding the occurrence of the transfer of acyl group, and improving the accuracy of the analysis results. Also, the use of the anhydrous ethanol could effectively avoid the generation of fatty acid as a by-product of hydrolysis, and simplify subsequent analysis steps.