Abstract: The present invention discloses an enzymatic synthesis method of 1-palmitic acid-2-oleic acid-3-triglyceride stearate, belonging to the technical field of structured lipid processing. In the present invention, a crude product rich in 1-palmitic acid-2-oleic acid-3-triglyceride stearate is obtained by reacting for 2-10 h at 20-70° C. with 1-palmitic acid-2-diglyceride oleate as an acyl acceptor, stearic acid or a derivative thereof as an acyl donor and a lipase as a catalyst, wherein the reaction system is an inorganic or organic solvent reaction system. The present invention provides a highly efficient enzymatic synthesis method of POS, thereby resolving the disadvantage of low POS content and limited use provided by the current synthesizing product of structured lipid. The technology adopted by the present invention certainly has an application prospect in the process of preparing cocoa butter equivalents.

Abstract: A method for enzymatically preparing a n-3 polyunsaturated fatty acid diacylglycerol using oil rich in n-3 polyunsaturated fatty acid as raw material to prepare diacylglycerol oil with selective enzymatic hydrolysis. Before the enzymatic reaction, the raw materials are homogenized to improve the catalytic efficiency of lipase in the hydrolysis and achieve the purpose of reducing the reaction time and the addition amount of lipase. As a result, the cost of industrial diacylglycerol preparation is reduced, ultimately reducing the cost of industrial diacylglycerol preparation. Therefore, the present invention provides an effective, mild and low-cost enzymatic method for preparing diacylglycerol rich in n-3 polyunsaturated fatty acids, which has a promising potential for industrial application.

Abstract: A method for enzymatically preparing a n-3 polyunsaturated fatty acid diacylglycerol using oil rich in n-3 polyunsaturated fatty acid as raw material to prepare diacylglycerol oil with selective enzymatic hydrolysis. Before the enzymatic reaction, the raw materials are homogenized to improve the catalytic efficiency of lipase in the hydrolysis and achieve the purpose of reducing the reaction time and the addition amount of lipase. As a result, the cost of industrial diacylglycerol preparation is reduced, ultimately reducing the cost of industrial diacylglycerol preparation. Therefore, the present invention provides an effective, mild and low-cost enzymatic method for preparing diacylglycerol rich in n-3 polyunsaturated fatty acids, which has a promising potential for industrial application.

Abstract: A conical workpiece length measurement method is provided. Two laser displacement sensors are symmetrically arranged at opposite sides of a to-be-measured conical workpiece or a tooling loaded with the to-be-measured conical workpiece. Distance X0 from each displacement sensor to a bottom plane of the to-be-measured conical workpiece is calibrated. An elongated base plate is arranged at a tip of the to-be-measured conical workpiece, and the two displacement sensors measure their respective distances to the base plate. The total length of the to-be-measured conical workpiece is calculated as follows: X=X0+(X1+X2)/2, where X1 represents distance from one of the two displacement sensors to the base plate, and X2 represents distance from the other of the two displacement sensors to the base plate. Factors influencing the length measurement include calibration of the fixed length, measurement accuracy of the displacement sensor and a tilt error of the base plate.

Abstract: A device for measuring length and center of mass of conical workpiece, including an inner frame, an outer frame, a base plate, two laser displacement sensors and a plurality of weighing mechanisms. The inner frame is rotatably connected with the outer frame through a pin shaft. A bottom surface of the conical workpiece can be fixedly connected with an inner bottom surface of the inner frame. The base plate is removably arranged on the inner frame, and abuts against a top of the conical workpiece. The base plate is perpendicular to an axis of the conical workpiece. The laser displacement sensors are fixedly arranged at a position of the inner frame below the top of the conical workpiece. The weighing mechanisms are provided at a bottom of the outer frame, and can bear the inner frame, the outer frame and the conical workpiece.

Abstract: A conical workpiece length measurement method is provided. Two laser displacement sensors are symmetrically arranged at opposite sides of a to-be-measured conical workpiece or a tooling loaded with the to-be-measured conical workpiece. Distance X0 from each displacement sensor to a bottom plane of the to-be-measured conical workpiece is calibrated. An elongated base plate is arranged at a tip of the to-be-measured conical workpiece, and the two displacement sensors measure their respective distances to the base plate. The total length of the to-be-measured conical workpiece is calculated as follows: X=X0+(X1+X2)/2, where X1 represents distance from one of the two displacement sensors to the base plate, and X2 represents distance from the other of the two displacement sensors to the base plate. Factors influencing the length measurement include calibration of the fixed length, measurement accuracy of the displacement sensor and a tilt error of the base plate.

Abstract: A device for measuring length and center of mass of conical workpiece, including an inner frame, an outer frame, a base plate, two laser displacement sensors and a plurality of weighing mechanisms. The inner frame is rotatably connected with the outer frame through a pin shaft. A bottom surface of the conical workpiece can be fixedly connected with an inner bottom surface of the inner frame. The base plate is removably arranged on the inner frame, and abuts against a top of the conical workpiece. The base plate is perpendicular to an axis of the conical workpiece. The laser displacement sensors are fixedly arranged at a position of the inner frame below the top of the conical workpiece. The weighing mechanisms are provided at a bottom of the outer frame, and can bear the inner frame, the outer frame and the conical workpiece.

Abstract: The present invention discloses an enzymatic synthesis method of 1-palmitic acid-2-oleic acid-3-triglyceride stearate, belonging to the technical field of structured lipid processing. In the present invention, a crude product rich in 1-palmitic acid-2-oleic acid-3-triglyceride stearate is obtained by reacting for 2-10 h at 20-70° C. with 1-palmitic acid-2-diglyceride oleate as an acyl acceptor, stearic acid or a derivative thereof as an acyl donor and a lipase as a catalyst, wherein the reaction system is an inorganic or organic solvent reaction system. The present invention provides a highly efficient enzymatic synthesis method of POS, thereby resolving the disadvantage of low POS content and limited use provided by the current synthesizing product of structured lipid. The technology adopted by the present invention certainly has an application prospect in the process of preparing cocoa butter equivalents.

Abstract: This invention discloses a method for improving n-3 polyunsaturated fatty acid enrichment efficiency in glyceride, belonging to the field of deep processing of oil. The method of this invention includes providing oil, lipase and metal ionic liquid into a reactor. After a reaction time, a mixture is obtained and free fatty acid is removed from the mixture, and the product is the n-3 polyunsaturated fatty acid-rich glyceride. Compared with the traditional chemical method, the method of this invention is characterized by mild reaction conditions and producing lower level of by-products. Meanwhile, metal ions selected in the present invention are able to promote the hydrolysis of lipase, solving the problem of long reaction time in the conventional enzymatic method. Rapid hydrolysis is achieved by this method so as to realize enrichment of EPA and DHA in glyceride.

Abstract: The present disclosure discloses a method for increasing the usage efficiency of a lipase in the enzymatic reaction, belonging to the technical field of food processing. In the present disclosure, the peroxide value of raw materials required for enzymatic synthesis of a structured lipid is reduced by a distillation method or an adsorption method, and then the raw material and a lipase undergo catalytic reaction to synthesize the structured lipid, so as to maintain the activity of the lipase and increase the reuse rate of the enzyme. The distillation method includes deodorization and molecular distillation. The present disclosure provides a method for manufacturing a structured lipid by a high efficiency and low-cost enzymatic synthesis, addressing to the issues such as large enzyme consumption, expansive cost, low enzyme utilization rate in the traditional industrial production. Therefore, the method provided by the present disclosure has certain development potential and practical significance.

Abstract: A method and an apparatus for measuring mass based on torque equilibrium, including a main blade for supporting and balancing a lever and parts arranged on the lever; a displacement measuring device for measuring a position of the lever; an electromagnetic torquer for balancing the apparatus after a balance weight is coarsely loaded during measuring; a product tooling on which a product to be measured is arranged and fixedly connected. The product tooling is fixed on a slider which freely slides along a guide rail; a locking block is provided to lock the product after the product slides in place; the guide rail is fixed on the level; a laser displacement sensor is provided to measure a displacement of the product along the guide rail; a lifting mechanism is provided to lift the lever and parts arranged on the lever to separate from the main blade.

Abstract: The present invention provides an enzymatic method for producing a fatty acid bornyl ester including using borneol and a fatty acid as a substrate for reaction and adding a lipase in a solvent system or a solvent-free system to catalyze the esterification reaction for a period of time to obtain fatty acid bornyl ester. The present method preferably uses fatty acids or their derivatives as acyl donors to prepare fatty acid bornyl esters. By utilizing the characteristics of the substrate, the synthesis process is simple; the reaction efficiency is high; and the content of fatty acid bornyl ester is up to 97%.

Abstract: This invention discloses a method for improving n-3 polyunsaturated fatty acid enrichment efficiency in glyceride, belonging to the field of deep processing of oil. The method of this invention includes providing oil, lipase and metal ionic liquid into a reactor. After a reaction time, a mixture is obtained and free fatty acid is removed from the mixture, and the product is the n-3 polyunsaturated fatty acid-rich glyceride. Compared with the traditional chemical method, the method of this invention is characterized by mild reaction conditions and producing lower level of by-products. Meanwhile, metal ions selected in the present invention are able to promote the hydrolysis of lipase, solving the problem of long reaction time in the conventional enzymatic method. Rapid hydrolysis is achieved by this method so as to realize enrichment of EPA and DHA in glyceride.

Abstract: The present invention provides an application of tilapia oil or fractionated product thereof, which belongs to the technical field of food technology. The applications involve in swine feed, fat for replacing breast milk, or dairy products. The present invention found that palmitic acid, oleic acid and linoleic acid in tilapia oil, and more than 65% of palmitic acid is distributed in sn-2 position, the highest content of triglyceride OPL, can be used as fat additives in swine feed or formula milk powder use oil. Therefore, adding tilapia oil to swine feed or formula milk powder is beneficial to the absorption of fatty acids and calcium, and can improve the absorption and utilization of energy.

Abstract: The present invention provides an enzymatic method for producing a fatty acid bornyl ester including using borneol and a fatty acid as a substrate for reaction and adding a lipase in a solvent system or a solvent-free system to catalyze the esterification reaction for a period of time to obtain fatty acid bornyl ester. The present method preferably uses fatty acids or their derivatives as acyl donors to prepare fatty acid bornyl esters. By utilizing the characteristics of the substrate, the synthesis process is simple; the reaction efficiency is high; and the content of fatty acid bornyl ester is up to 97%.

Abstract: A method and an apparatus for measuring mass based on torque equilibrium, including a main blade for supporting and balancing a lever and parts arranged on the lever; a displacement measuring device for measuring a position of the lever; an electromagnetic torquer for balancing the apparatus after a balance weight is coarsely loaded during measuring; a product tooling on which a product to be measured is arranged and fixedly connected. The product tooling is fixed on a slider which freely slides along a guide rail; a locking block is provided to lock the product after the product slides in place; the guide rail is fixed on the level; a laser displacement sensor is provided to measure a displacement of the product along the guide rail; a lifting mechanism is provided to lift the lever and parts arranged on the lever to separate from the main blade.

Abstract: The present invention provides a method for vegetable oil deacidification by enzymatic amidation, which relates to the field of oil refining technology. The present invention is carried out through mixing high acid value vegetable oil with ethanolamine at a certain molar ratio in a solvent or solvent-free system, adding with a certain amount of lipase, and reacting at certain temperature for a period of time. The monoethanolamine has been used as an acyl donor for the first time to react with free fatty acid, which avoids the increasing amount of by-products and great loss of neutral oil in reaction that involved with triglycerides. The method of the present invention has the advantages of high selectivity, high catalytic efficiency, and environment friendly in the reaction. From enzymes recycling, it greatly reduces costs, which shows tremendous potential in the application.

Abstract: The present invention provides a method for vegetable oil deacidification by enzymatic amidation, which relates to the field of oil refining technology. The present invention is carried out through mixing high acid value vegetable oil with ethanolamine at a certain molar ratio in a solvent or solvent-free system, adding with a certain amount of lipase, and reacting at certain temperature for a period of time. The monoethanolamine has been used as an acyl donor for the first time to react with free fatty acid, which avoids the increasing amount of by-products and great loss of neutral oil in reaction that involved with triglycerides. The method of the present invention has the advantages of high selectivity, high catalytic efficiency, and environment friendly in the reaction. From enzymes recycling, it greatly reduces costs, which shows tremendous potential in the application.

Abstract: The present invention relates to methods for making a fatty acid N-acylalkanolamine having the formula: One of the methods comprises the steps of providing a vinyl ester of a fatty acid having the formula: providing a primary or secondary alkanolamine having the formula: NHR1R2; and reacting the vinyl ester of the fatty acid with the alkanolamine under conditions effective to form the fatty acid N-acylalkanolamine. The other method comprises the steps of providing a fatty acid; purifying the fatty acid; providing a primary or secondary alkanolamine having the formula: NHR1R2; reacting the fatty acid with the alkanolamine under conditions effective to form the fatty acid N-acylalkanolamine; and purifying the formed fatty acid N-acylalkanolamine.

Abstract: The present invention relates to methods for making a fatty acid N-acylalkanolamine having the formula: One of the methods comprises the steps of providing a vinyl ester of a fatty acid having the formula: providing a primary or secondary alkanolamine having the formula: NHR1R2; and reacting the vinyl ester of the fatty acid with the alkanolamine under conditions effective to form the fatty acid N-acylalkanolamine. The other method comprises the steps of providing a fatty acid; purifying the fatty acid; providing a primary or secondary alkanolamine having the formula: NHR1R2; reacting the fatty acid with the alkanolamine under conditions effective to form the fatty acid N-acylalkanolamine; and purifying the formed fatty acid N-acylalkanolamine.