Abstract: An application of carbasalate calcium in preparing the drug for the treatment of hydrocephalus is provided, and the drug uses carbasalate calcium as the only or main active ingredient. The invention first discovered the effect of carbasalate calcium in the treatment of hydrocephalus, which can significantly reduce the area of the fourth ventricle of the zebrafish model of hydrocephalus, and significantly increase the swimming distance, swimming time, swimming speed, and rotational motion frequency of the zebrafish model of hydrocephalus; the experimental data provided by the invention show that carbasalate calcium has great application prospects in the treatment of hydrocephalus, and provides an effective candidate drug for alleviating the pain of hydrocephalus patients and reducing the risk and cost of treatment; it broadens the scope of action of carbasalate calcium.

Abstract: An application of Bone Marrow Mesenchyml Stem Cell Exosomes (BMSC-Exos) in treating Parkinson's disease (PD) is provided, wherein the BMSC-Exos are generated by stimulating BMSCs with a culture solution and extracted from the culture solution after passage; and the culture solution of the BMSCs is an ?-MEM culture solution containing FBS and PS. The BMSC-Exos can greatly improve a motor function of a model mouse with PD, protect dopaminergic neurons of the model mouse with PD, improve an olfactory function of the model mouse with PD, and also inhibit the activation of olfactory astrocytes of the model mouse with PD.

Abstract: A traditional Chinese medicine compound preparation for treating Parkinson's disease and its preparation method and application are disclosed. The traditional Chinese medicine compound preparation comprises traditional Chinese medicine formula components and the traditional Chinese medicine formula components comprise the following raw material or raw material extracts by mass: 6-15 parts of uncaria rhynchophylla, 3-9 parts of gastrodia elata, 6-12 parts of Gentiana macrophylla, 10-20 parts of raw Astragalus membranaceus, 10-20 parts of prepared rehmannia roots, 10-20 parts of achyranthes roots, 10-20 parts of wolfberry fruits, 10-20 parts of angelica sinensis, 10-20 parts of fried atractylodes macrocephala, 6-15 parts of fried spina date seeds, 3-12 parts of walnut kernels, 3-9 parts of safflower, and 20-30 parts of zaocys dhumnade.

Abstract: A recombinant human collagen and a method for building same are provided. The amino acid sequence of the recombinant human collagen is SEQ ID NO. 1: GPAGARGNDGATGAAGPPGPTGPAGPPGFP. The recombinant human collagen does not have a signal peptide and a transmembrane domain and is a novel hydrophilic protein. In addition, the protein does not have an antigenic determinant and does not elicit an immunological rejection response when applied to human body. The present invention further discloses a method for building and synthesizing a low-immunogenic hydrophilic recombinant human collagen. The recombinant human collagen synthesized by using the present invention has high purity, no virus risk, and a high expression level. The protein can be widely applied to related biomedical products, regenerative medicine products, tissue engineering and beauty products, health care products, cosmetic products, among others.

Abstract: A silk fibroin nerve graft fused with NT3 and a preparation method therefor is provided. The method includes the steps of: synthesizing a gene fragment containing a silk fibroin light chain and NT-3, connecting the fragment to a pET-30 expression vector, and transferring the obtained recombinant expression vector into BL21 Escherichia coli to obtain a fusion protein; placing a silk fibroin fiber web in a mold, mixing the fusion protein and a silk fibroin solution, and performing freeze drying to enable the silk fibroin to be crosslinked with the fusion protein to form a nerve conduit; and after deformation processing, finally obtaining a silk fibroin nerve graft having NT-3 activity. The silk fibroin nerve graft can provide a mechanical support, exert nerve protection and nerve regeneration promotion functions in a long term, and adjust the proportion of NT-3 bioactive peptides in the nerve conduit, facilitating repair of nerve injuries.

Abstract: A bionic nerve graft and a preparation method thereof is provided. The bionic nerve graft includes a helical stent, a nanofiber membrane, guide fibers and hBMSC-ECM. A surface of the helical stent is coated with the nanofiber membrane, the guide fibers and the hBMSC-ECM are placed in a tube cavity of the helical stent, and the raw materials of the helical stent, the nanofiber membrane and the guide fibers are all chitosan. The preparation method includes the following steps: preparing the helical stent by a mold pouring method, coating the surface of the helical stent with the nanofiber membrane by an electrostatic spinning method, preparing the guide fibers by a template method, and filling the guide fibers and the hBMSC-ECM in the helical stent with the surface coated with the nanofiber membrane.

Abstract: Provided are a Forsythia suspensa and Radix astragali compound traditional Chinese medicine decoction-derived micro ribonucleic acid and a preparation method therefor. The micro ribonucleic acid is selected from miRNAs with nucleotide sequences as shown in SEQ ID NOs. 1-15. Further provided are a miRNA QQ_159 with the nucleotide sequence as shown in SEQ ID NO. 14, comprising an artificially synthesized QQ_159, a plant QQ_159, a precursor form of the QQ_159, or a mature form of the QQ_159, use of the miRNA QQ_159 in the preparation of a drug for treating viral pneumonia caused by viral influenza and SARS-COV-2 virus, and a pharmaceutical composition comprising the miRNA QQ_159 and a pharmaceutically acceptable carrier.

Abstract: A cell matrix nerve graft for repairing peripheral nerve injury and a preparation method thereof are provided. The cell matrix nerve graft comprises acellular matrix and a scaffold, wherein the acellular matrix is obtained by decellularization after secretion and formation of stem cells, and the acellular matrix is wrapped around the scaffold. The defects of autologous nerve transplantation repair can be overcome and the immunogenicity of allogeneic cell transplantation repair is avoided. The cell matrix nerve graft beneficial to nerve cell proliferation and migration is provided, a microenvironment beneficial to axon growth is established, the purposes of nerve regeneration and function repair are achieved, and a feasible solution is provided for clinical treatment of nerve defects.

Abstract: An application of Bone Marrow Mesenchyml Stem Cell Exosomes (BMSC-Exos) in treating Parkinson's disease (PD) is provided, wherein the BMSC-Exos are generated by stimulating BMSCs with a culture solution and extracted from the culture solution after passage; and the culture solution of the BMSCs is an ?-MEM culture solution containing FBS and PS. The BMSC-Exos can greatly improve a motor function of a model mouse with PD, protect dopaminergic neurons of the model mouse with PD, improve an olfactory function of the model mouse with PD, and also inhibit the activation of olfactory astrocytes of the model mouse with PD.

Abstract: A multi-disulfide-bond long-chain peptide with neuroprotective activity can reduce cellular calcium influx by inhibiting glutamate receptors to protect cortical neurons from excitotoxicity induced by glutamate. It can be an effective neuroprotective agent.

Abstract: Disclosed are a compound preparation for neuranagenesis, and a preparation method therefor and the use thereof. The compound preparation for neuranagenesis comprises the raw medicinal materials in parts by weight: 10-20 parts of raw Astragali radix, 10-20 parts of Rehmaimiae Radix praeparata, 10-20 parts of Actyranthes bidentata, 6-15 parts of Jujubae fructus, 6-15 parts of Lycii fructus, 6-15 parts of parched Ziziphi spinosae semen, 6-12 parts of Angelicae smensis radix, 3-9 parts of Carthami flos, 6-15 parts of Poria, 6-15 parts of parched Atractylodis macrocephalae rhizoma and 10-20 parts of Zaocys. The compound preparation can be used for preparing drugs to treat nerve damage diseases, and can be prepared into oral liquids, granules, dissolved granules and tablets.

Abstract: A Fructus Forsythiae and Radix Astragali compound preparation, and a preparation method therefor and a use thereof is provided. Traditional Chinese medicine formulation components consist of the following raw materials or raw material extracts in parts by mass: 9-11 parts of Flos Lonicerae, 9-11 parts of Fructus Forsythiae, 9-11 parts of Radix Scutellariae, 9-11 parts of Herba Artemisiae Annuae, 9-11 parts of Radix Astragali, 9-11 parts of stir-fried Rhizoma Atractylodis Macrocephalae, 9-11 parts of Herba Pogostemonis, 5-7 parts of Radix Saposhnikoviae, 9-11 parts of Radix Ophiopogonis, and 5-7 parts of Radix Glycyrrhizae. The components can be made into an oral liquid, a granule, a dissolved medicine, or a tablet. Further disclosed is a use of the Fructus Forsythiae and Radix Astragali compound preparation in preparation of medicines for preventing and/or treating a viral influenza disease.

Abstract: A protein-modified PLGA microsphere can be used to construct tissue-engineered nerve. The microspheres are loaded with active substances for treating peripheral nerve injury and are bound to tissue-engineered nerves. It has been shown that the prepared tissue-engineered nerve effectively promotes nerve regeneration after peripheral nerve injury.

Abstract: Ag—Fe3O4 immunomagnetic microsphere contains poly-D-lysine modified on the surface and S100? and/or MBP antibody linked by an amide bond. The Ag—Fe3O4 immunomagnetic microsphere can specifically capturing peripheral nerve tissue-derived exosomes. When the microsphere is used to extract nerve tissue-derived exosomes, the extraction yield of exosomes per unit volume of nerve tissue is high, and the nerve specificity is strong.

Abstract: A traditional Chinese medicine compound preparation for treating Parkinson's disease and its preparation method and application are disclosed. The traditional Chinese medicine compound preparation comprises traditional Chinese medicine formula components and the traditional Chinese medicine formula components comprise the following raw material or raw material extracts by mass: 6-15 parts of uncaria rhynchophylla, 3-9 parts of gastrodia elata, 6-12 parts of Gentiana macrophylla, 10-20 parts of raw astragalus membranaceus, 10-20 parts of prepared rehmannia roots, 10-20 parts of achyranthes roots, 10-20 parts of wolfberry fruits, 10-20 parts of angelica sinensis, 10-20 parts of fried atractylodes macrocephala, 6-15 parts of fried spina date seeds, 3-12 parts of walnut kernels, 3-9 parts of safflower, and 20-30 parts of zaocys dhumnade.

Abstract: A multi-disulfide-bond long-chain peptide with neuroprotective activity can reduce cellular calcium influx by inhibiting glutamate receptors to protect cortical neurons from excitotoxicity induced by glutamate. It can be an effective neuroprotective agent.

Abstract: A silk fibroin nerve graft fused with NT3 and a preparation method therefor is provided. The method includes the steps of: synthesizing a gene fragment containing a silk fibroin light chain and NT-3, connecting the fragment to a pET-30 expression vector, and transferring the obtained recombinant expression vector into BL21 Escherichia coli to obtain a fusion protein; placing a silk fibroin fiber web in a mold, mixing the fusion protein and a silk fibroin solution, and performing freeze drying to enable the silk fibroin to be crosslinked with the fusion protein to form a nerve conduit; and after deformation processing, finally obtaining a silk fibroin nerve graft having NT-3 activity. The silk fibroin nerve graft can provide a mechanical support, exert nerve protection and nerve regeneration promotion functions in a long term, and adjust the proportion of NT-3 bioactive peptides in the nerve conduit, facilitating repair of nerve injuries.

Abstract: A Fructus Forsythiae and Radix Astragali compound preparation, and a preparation method therefor and a use thereof is provided. Traditional Chinese medicine formulation components consist of the following raw materials or raw material extracts in parts by mass: 9-11 parts of Flos Lonicerae, 9-11 parts of Fructus Forsythiae, 9-11 parts of Radix Scutellariae, 9-11 parts of Herba Artemisiae Annuae, 9-11 parts of Radix Astragali, 9-11 parts of stir-fried Rhizoma Atractylodis Macrocephalae, 9-11 parts of Herba Pogostemonis, 5-7 parts of Radix Saposhnikoviae, 9-11 parts of Radix Ophiopogonis, and 5-7 parts of Radix Glycyrrhizae. The components can be made into an oral liquid, a granule, a dissolved medicine, or a tablet. Further disclosed is a use of the Fructus Forsythiae and Radix Astragali compound preparation in preparation of medicines for preventing and/or treating a viral influenza disease.

Abstract: Disclosed are a compound preparation for neuranagenesis, and a preparation method therefor and the use thereof. The compound preparation for neuranagenesis comprises the raw medicinal materials in parts by weight: 10-20 parts of raw Astragali radix, 10-20 parts of Rehmaimiae Radix praeparata, 10-20 parts of Actyranthes bidentata, 6-15 parts of Jujubae fructus, 6-15 parts of Lycii fructus, 6-15 parts of parched Ziziphi spinosae semen, 6-12 parts of Angelicae smensis radix, 3-9 parts of Carthami flos, 6-15 parts of Poria, 6-15 parts of parched Atractylodis macrocephalae rhizoma and 10-20 parts of Zaocys. The compound preparation can be used for preparing drugs to treat nerve damage diseases, and can be prepared into oral liquids, granules, dissolved granules and tablets.

Abstract: Ag-Fe3O4 immunomagnetic microsphere contains poly-D-lysine modified on the surface and S100? and/or MBP antibody linked by an amide bond. The Ag-Fe3O4 immunomagnetic microsphere can specifically capturing peripheral nerve tissue-derived exosomes. When the microsphere is used to extract nerve tissue-derived exosomes, the extraction yield of exosomes per unit volume of nerve tissue is high, and the nerve specificity is strong.