Abstract: A conjugate for active targeted therapy of tumors is obtained by coupling a hydrophilic affibody with an active tumor targeting function and a hydrophobic anti-tumor cytotoxin through a small molecule linker. The affibody has a specific binding activity and a high affinity to receptors overexpressed on surfaces of various tumor cells. The cytotoxin is a highly effective anti-tumor drug. The affibody-cytotoxin conjugate can self-assemble in water to form the nanoparticle with the affinity body as a shell and the cytotoxin as an inner core. Since the affibody can specifically recognize and bind to receptors overexpressed on the surfaces of the tumor cells, the nanoparticle can be efficiently enriched at tumor sites and biodegraded through the linker to release the cytotoxin and reach effective therapeutic concentrations for inhibiting the growth of the tumors while reducing toxic and side effects to normal organs and tissues.

Abstract: The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications.

Abstract: Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications.

Abstract: The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications.

Abstract: A high-molecular-weight recombinant silk or silk-like protein having a molecular weight which is substantially similar to that of native silk protein, and a micro- or nano-sized spider silk or silk-like fiber having improved physical properties, produced therefrom. The recombinant silk or silk-like protein according to the invention has high molecular weight, like dragline silk proteins from spiders, while a fiber produced therefrom has excellent physical properties compared to a fiber produced from native silk protein. Thus, the recombinant silk or silk-like protein and the spider silk or silk-like fiber produced therefrom will be highly useful in various industrial applications, including bioengineering applications and medical applications.

Abstract: The present invention relates to a method of increasing the expression of a target protein by co-expression of a gene encoding a target protein having a high content of a specific amino acid with a nucleotide sequence encoding the tRNA of the specific amino acid. According to the present invention, the expression of a protein having a high content of a specific amino acid can be remarkably increased by co-expression with the tRNA of the specific amino acid. Thus, the present invention is useful for increasing the productivity of a protein having a high content of a specific amino acid, such as a repetitive protein.

Abstract: Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications.