Abstract: The present invention relates to a specific TNFR2 binding molecule, an epitope peptide of TNFR2 that is bound to the TNFR2 binding molecule, and a composition containing same. The present invention also relates to a nucleic acid encoding the TNFR2 binding molecule, a host cell containing the nucleic acid, and a method for preparing the TNFR2 binding molecule. Furthermore, the present invention relates to the therapeutic and diagnostic use of the TNFR2 binding molecules. Particularly, the present invention relates to the combined treatment of the TNFR2 binding molecules with other therapies, such as therapeutic methods or therapeutic agents.

Abstract: The present disclosure relates to a two-plasmid system for prime editing in yeast, use thereof, and a method for gene prime editing in yeast. The two-plasmid system includes a first plasmid and a second plasmid. The first plasmid includes a sequence encoding for an epegRNA. The epegRNA is an RNA molecule including a motif at a 3?-terminus of a pegRNA, and the motif having a sequence as set forth in SEQ ID NO. 12. The second plasmid includes a sequence encoding for a fusion protein of a nucleic acid nickase nCas9 fused with a reverse transcriptase M-MLV RT. The two-plasmid expression system can be used in gene editing in yeast.