Abstract: A dual-probe method for fluorescence quantitative PCR (Polymerase Chain Reaction) is disclosed. The method involves the use of a Taqman dual-probe detection system with the same pair of primers. The dual probes includes a detection probe and a reference probe. The detection probe targets the wild-type sequence at the hotspot mutation site, which can cover multiple adjacent mutations. The reference probe targets the wild-type sequence adjacent to the target sequence. Both probes share the same pair of upstream and downstream primers. This method uses a single reaction qPCR (quantitative PCR) method, which can simultaneously detect multiple adjacent mutations. It effectively saves tissue samples and greatly shortens the testing period. Moreover, the close proximity of the target and reference sequences enhances the reliability and accuracy of the results, making it widely applicable in various fields.

Abstract: The disclosure provides a kit for detecting microsatellite instability and a method therefor. The kit includes a negative control, a plurality of qPCR reaction solutions, a qPCR premix and a sterile enzyme-free water; the plurality of qPCR reaction solutions includes 6 pairs of upstream primers and downstream primers of which the MSI mutation site is amplified, and a reference probe for the internal reference and a detection probe for the mutation site. The difference between the amplification of the gene and the gene at the mutation site of the samples and the negative control is used to detect the microsatellite instability. The method and kit as provided is easy and simple without the need of normal tissues being a control, and the need to open the cap. By doing so, aerosol pollution is avoided and sample supplies are conserved.

Abstract: Methods, apparatuses, electronic devices, and storage media for makeup look processing are provided. In one aspect, a method includes: displaying a collected face image; in response to a selection of a makeup look from one or more makeup looks, identifying a face part area matching the makeup look from the face image and providing an instruction for a making-up content for the face part area; detecting, from an updated collected face image, pixel change information of the face part area; and determining whether the pixel change information of the face part area meets a makeup effect condition for the first makeup look.