Abstract: An SNP marker significantly related with yardlong bean anthocyanin content is provided in the present disclosure. The SNP marker significantly related with yardlong bean anthocyanin content is located at the position of 2,361,292 bp on chromosome 5 of yardlong bean genome v1.2, with a base A to G substitution, and the nucleotide sequence thereof is shown in SEQ ID NO. 1. KASP marker based on the SNP marker significantly related with the yardlong bean anthocyanin content and application thereof are also provided in the present disclosure. The KASP marker and the SNP marker significantly related with yardlong bean anthocyanin content are applied for molecular marker-assisted selection breeding of yardlong bean anthocyanin traits.

Abstract: Disclosed are an application of a plant TIM protein, a TIM gene encoding the plant TIM protein or a homologous gene of the same in plant resistance to viruses. Further disclosed is a method for obtaining virus-resistant plant, where the method involves downregulating the expression or activity of the plant TIM protein. Further disclosed is a method for identifying whether the plants obtained by the above method has antiviral capability, to determine whether the plant contains TIM protein or TIM genes through identification. The present disclosure introduces a gene editing vector and a gene silencing vector carrying a target gene into target plant using a transgenic method, so as to obtain homozygous plant with GmTIM gene knockout in soybean and plant with down-regulated expression of NbTIM in Nicotiana benthamiana, which are capable of significantly inhibiting viral infection.

Abstract: Provided is a soybean oligosaccharide-related kompetitive allele-specific PCR (KASP) marker and use thereof, belonging to the technical field of molecular breeding. The KASP marker includes one or two of S18_51868868 T/G and S10_38081012 T/C, where the S18_51868868 T/G is a base T or a base G at a 51868868bp position on chromosome 18 of a soybean genome; and the S10_38081012 T/C is a base T or a base C at a 38081012bp position of chromosome 10 of the soybean genome. In the present disclosure, the KASP marker can accurately genotype the traits of raffinose and stachyose contents. Through genotyping, it is found that a soybean germplasm with a genotype GG has a higher raffinose content than that of a soybean germplasm with a genotype TT, and a soybean germplasm with a genotype TT has a higher stachyose content than that of a soybean germplasm with a genotype CC.

Abstract: An SNP marker significantly related with yardlong bean anthocyanin content is provided in the present disclosure. The SNP marker significantly related with yardlong bean anthocyanin content is located at the position of 2,361,292 bp on chromosome 5 of yardlong bean genome v1.2, with a base A to G substitution, and the nucleotide sequence thereof is shown in SEQ ID NO. 1. KASP marker based on the SNP marker significantly related with the yardlong bean anthocyanin content and application thereof are also provided in the present disclosure. The KASP marker and the SNP marker significantly related with yardlong bean anthocyanin content are applied for molecular marker-assisted selection breeding of yardlong bean anthocyanin traits.