Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures such as rolling circle amplification products in the biological sample.

Abstract: The present disclosure relates in some aspects to methods and compositions for analysis of a target nucleic acid, such as in situ detection of a region of interest in a polynucleotide in a tissue sample. In some embodiments, provided herein are templated ligation probes (e.g., RNA-templated ligation probes) and selector probes for generation of a circularized ligated probe comprising an insertion sequence of a selector probe, wherein the circularized ligated probe is amplified in a rolling circle amplification reaction to generate a product that is detected in the sample.

Abstract: The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, provided herein are methods and compositions for detecting a region of interest in a target nucleic acid.

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein allow for detection of a target sequence by rolling circle amplification without requiring a ligation step and without sacrificing specificity (e.g., rolling circle amplification occurs only for circular probes and/or hairpin molecules specifically hybridized to a target sequence).

Abstract: In some aspects, the present disclosure relates to methods for reducing the crowding of signals, for example optical crowding, that can occur when nucleic acids are detected in a sample in multiplex, which can make it difficult to resolve individual signals and can lead to a reduced dynamic range. In some aspects, the present disclosure relates to methods for reducing signal crowding in the detection of multiple target nucleic acid sequences in a sample, e.g., using hybridization probes, wherein signal crowding from said hybridization probes is reduced. The methods herein have particular applicability in the detection of barcode sequences by sequencing-by-hybridization (SBH) methods, including those relying on combinatorial labelling schemes and decoding of the barcodes by sequential cycles of decoding using hybridization probes. Also provided are kits comprising probes for use in such methods.

Abstract: The present disclosure provides a foldable drain pad for a faucet. The back of the drain pad is provided with a bent groove, so that the drain pad can form a bottom plate and a folded plate which can be bent along the bent groove. The bottom plate is provided with a through hole and a connecting groove which is connected with the through hole, so that the faucet can enter along the path of the connecting groove and be installed at the through hole. A plurality of sealing fins are formed on the peripheral surface of the through hole on the bottom plate, and a U-shaped connecting part is arranged between two adjacent sealing fins, so that the sealing fins can wrap the pipe wall of the faucet in all directions by folding. There is a U-shaped connecting part between the sealing fins, and the joint action of the sealing fins and the U-shaped connecting part can realize the omni-directional wrapping function of the pipe wall of the faucet, so that the table top of the washstand is dry and sanitary.

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying analytes present at high levels, such as highly expressed genes in a sample. In some embodiments, a probe-resolution barcode sequence disclosed herein does not specifically correspond to any particular target analyte(s) but can be used to resolve dense optical signals due to spatially overlapping signals associated with different molecules of a target analyte, thereby enabling resolution of signals in a dense “spot” and accurate counting of spots associated with molecules that are in spatial proximity. Also provided are kits comprising probes for use in such methods.

Abstract: In some aspects, the present disclosure relates to methods for reducing the crowding of signals, for example optical crowding, that can occur when nucleic acids are detected in a sample in multiplex, which can make it difficult to resolve individual signals and can lead to a reduced dynamic range. In some aspects, the present disclosure relates to methods for reducing signal crowding in the detection of multiple target nucleic acid sequences in a sample, e.g., using hybridization probes, wherein signal crowding from said hybridization probes is reduced. The methods herein have particular applicability in the detection of barcode sequences by sequencing-by-hybridization (SBH) methods, including those relying on combinatorial labelling schemes and decoding of the barcodes by sequential cycles of decoding using hybridization probes. Also provided are kits comprising probes for use in such methods.

Abstract: A system and method are disclosed for providing secure deposit and recovery of secret data based on a secret of a user, such as a password, a shared secret from a recovery server, and a secret from a recovery peer. The secret data is encrypted with these three secrets and stored remote from the user device to only allow the user to recover the secret data without compromising the secrecy of the secret data. Systems and methods for decoupling a password from the secret data the password protects is also provided to allow resetting the password or recovering the secret data to be separate operations that can be carried out independently. Another aspect provides for a user account to be securely recovered using a recovery peer to verify ownership of the user account.

Abstract: A system and method are provided for updating database statistics for use in generating query execution plans. A first query for a database is received, for example by a compiler, and the compiler accesses statistics associated with the first query for optimizing a query execution plan for the first query. The statistics may be accessed from a first cache. If the statistics retrieved from the first cache are determined to be stale, the system obtains updated statistics and may store them in a temporary cache. The stale statistics in the first cache are marked as invalid. Existing queries using the first cache may continue to use the first cache, while new queries may be provided with updated statistics.

Abstract: For a data processing system having memory for storing a database, a method, a system and a computer program product for directing the data processing system to process a record to be inserted into the database is disclosed. The database includes a plurality of base tables.

Abstract: For a data processing system having memory for storing a database, a method, a system and a computer program product for directing the data processing system to process a record to be inserted into the database is disclosed. The database includes a plurality of base tables.

Abstract: A system and method are provided for updating database statistics for use in generating query execution plans. A first query for a database is received, for example by a compiler, and the compiler accesses statistics associated with the first query for optimizing a query execution plan for the first query. The statistics may be accessed from a first cache. If the statistics retrieved from the first cache are determined to be stale, the system obtains updated statistics and may store them in a temporary cache. The stale statistics in the first cache are marked as invalid. Existing queries using the first cache may continue to use the first cache, while new queries may be provided with updated statistics.

Abstract: For a data processing system having memory for storing a database, a method, a system and a computer program product for directing the data processing system to process a record to be inserted into the database is disclosed. The database includes a plurality of base tables.

Abstract: For a data processing system having memory for storing a database, a method, a system and a computer program product for directing the data processing system to process a record to be inserted into the database is disclosed. The database includes a plurality of base tables.

Abstract: For a data processing system having memory for storing a database, a method, a system and a computer program product for directing the data processing system to process a record to be inserted into the database is disclosed. The database includes a plurality of base tables.

Abstract: For a data processing system having memory for storing a database, a method, a system and a computer program product for directing the data processing system to process a record to be inserted into the database is disclosed. The database includes a plurality of base tables.