Abstract: A smart walking assistance device with a walker frame having generally vertical sides and an intersecting front. Wheels located at both ends of a bottom edge of the sides. A soft robotic sensing handle extends in a C shape along the upper edges of the sides and front. The sensing handle has multiple contiguous air filled chambers, each containing a pressure sensor for producing a pressure signal representing the pressure within the chamber. A microcontroller unit receives the pressure signals from the pressure sensors of the handle chambers and determines the status of at least one of the device and a user of the device based on the pressure signals. A stabilization mechanism is driven by the microcontroller so as to stabilize the walker in response to the determined status of at least one of the walker and the user.

Abstract: Disclosed herein are methods for harvesting a recombinant protein comprising an asparaginyl endopeptidase (AEP) cleavage site, as well as methods for inhibiting cleavage of a recombinant protein comprising an AEP cleavage site, during a biomanufacturing process.

Abstract: Subject matter related to data management is disclosed. Snapshots of a target object in a computing system may be stored, where the snapshot may correspond to multiple versions of the target object. An indication that information in one or more of the snapshots may be stored. A request to restore a snapshot of the target object may be received. In response to the request, a determination of whether the snapshot of the target object is prohibited from being restored may be made based on the indication that information in one or more of the snapshots is flagged for quarantine. A determination of whether to initiate a restoration operation in response to the request to restore the snapshot may also be made based on whether the snapshot is prohibited from being restored.

Abstract: Subject matter related to data management is disclosed. Snapshots of a target object in a computing system may be stored, where the snapshots may correspond to multiple versions of the target object. A first indication that information in a snapshot of the snapshots is flagged for quarantine may be obtained. Based on receiving the first indication, a second indication that the information in the snapshot is prohibited from being restored during a restoration operation for the target operation may be generated. The second indication that the information in the snapshot is prohibited from being restored may be stored.

Abstract: A smart walking assistance device with a walker frame having generally vertical sides and an intersecting front. Wheels located at both ends of a bottom edge of the sides. A soft robotic sensing handle extends in a C shape along the upper edges of the sides and front. The sensing handle has multiple contiguous air filled chambers, each containing a pressure sensor for producing a pressure signal representing the pressure within the chamber. A microcontroller unit receives the pressure signals from the pressure sensors of the handle chambers and determines the status of at least one of the device and a user of the device based on the pressure signals. A stabilization mechanism is driven by the microcontroller so as to stabilize the walker in response to the determined status of at least one of the walker and the user.

Abstract: Disclosed are methods of purifying a protein sample. More specifically, provide are methods of removing or reducing the amount of high molecular weight species and/or high mannose species from a protein sample using a mixed mode chromatography step and a hydrophobic interaction chromatography step.

Abstract: Disclosed are a movie data display method and device, including: when receiving an instruction of triggering first movie information of a user on an interface where a list of at least one piece of movie information is displayed, popping up a first popup window, wherein the first popup window includes a play time of the first movie information and a ticket amount corresponding to the play time; after receiving a ticket purchase instruction of the ticket amount corresponding to the first play time of the first movie information in the first popup window, popping up a second popup window for payment; and after receiving a ticket purchase confirmation instruction in the second popup window, obtaining live broadcast stream of the first movie information in a server according to an identifier of the first movie information, and displaying the live broadcast stream to the user at the first play time.

Abstract: The present invention relates to a method for harvesting recombinant proteins from mammalian cell culture fluid. The method makes use of cationic polymers, non-ionic polymers and non-ionic surfactants.

Abstract: The present invention relates to a method for harvesting recombinant proteins from mammalian cell culture fluid. The method makes use of cationic polymers, non-ionic polymers and non-ionic surfactants.

Abstract: The present invention relates to a method for harvesting recombinant proteins from mammalian cell culture fluid. The method makes use of cationic polymers, non-ionic polymers and non-ionic surfactants.

Abstract: Key genes, microRNAs, other non-coding RNAs and a combination thereof for identifying and regulating pluripotency of cells are provided. The key genes, microRNAs, other non-coding RNAs and a combination thereof are highly expressed in full pluripotent stem cells but are significantly suppressed or silenced in partially pluripotent stem cells. The genes, microRNAs and other non-coding RNAs are those of a chromosome imprinted Dlk1-Dio3 region located on the long arm of mouse chromosome 12, and are homologous genes, microRNAs and other non-coding RNAs having 70-100% homology with them in genomic syntenic regions of other mammals. Also provided are uses of the genes, microRNAs, other non-coding RNAs and combination thereof in identifying the pluripotent status of stem cells and regulating pluripotency of cells; in typing stem cells; regulating pluripotency of cells, pluripotent states and levels of cells; treating diseases; and developing drug targets for tumor treatment and antitumor drugs.

Abstract: Disclosed is a oxidation resistant composite reverse osmosis membrane, comprising a porous support and a polyamide layer, in which the polyamide layer is modified by an agent so that the membrane retains a salt rejection of at least 85% after being subjected to an aqueous solution of NaClO having a concentration of about 2,000 to 30,000 ppm for about one hour. A process of preparing the membrane is also disclosed.