Abstract: A Differential Scanning calorimetry (DSC) thermal analysis method for the action of an applied electric field includes: step 1, in an experiment module of a differential scanning calorimeter, placing a microelectrode crucible and a reference crucible on corresponding sensors, connecting electrode wires of the microelectrode crucible with a signal generator, setting signal parameters to be output, placing a tested sample in a gap between electrodes, closing a microelectrode crucible lid, and closing the experiment module; step 2, at a temperature-varying stage, measuring a DSC curve of the tested sample under the action of an electric field, and at a reheating stage, measuring a DSC curve of the tested sample with no electric field; and step 3, analyzing the DSC curves in combination with the related theories of dielectrics and thermodynamics, and calculating an electric field intensity of the tested sample and a phase transformation rate of the tested sample.

Abstract: A DSC (Differential Scanning calorimetry) electrode system capable of applying an electric field includes a differential scanning calorimeter, a computer, a signal generator, a self-pressurization liquid nitrogen tank and a reference crucible, wherein the self-pressurization liquid nitrogen tank is connected to the differential scanning calorimeter and used for controlling temperature in real time; the differential scanning calorimeter is connected to the computer and used for transmitting signals and recording experiment results. The DSC electrode system also includes a microelectrode crucible that includes a ceramic crucible, a ceramic crucible cover, welding spots, two electrodes and electrode wires, wherein the two electrodes are respectively fixed in the ceramic crucible; a gap is reserved between the electrodes and used for storing a tested sample; the welding spots are reserved at upper ends of the electrodes.

Abstract: A Differential Scanning calorimetry (DSC) thermal analysis method for the action of an applied electric field includes: step 1, in an experiment module of a differential scanning calorimeter, placing a microelectrode crucible and a reference crucible on corresponding sensors, connecting electrode wires of the microelectrode crucible with a signal generator, setting signal parameters to be output, placing a tested sample in a gap between electrodes, closing a microelectrode crucible lid, and closing the experiment module; step 2, at a temperature-varying stage, measuring a DSC curve of the tested sample under the action of an electric field, and at a reheating stage, measuring a DSC curve of the tested sample with no electric field; and step 3, analyzing the DSC curves in combination with the related theories of dielectrics and thermodynamics, and calculating an electric field intensity of the tested sample and a phase transformation rate of the tested sample.

Abstract: An isolated DNA molecule which includes a promoter-effective region of a Tetrahymena metallothionein gene, as well as a chimeric gene, expression vectors, and host cells containing the same. Also disclosed is a method of expressing an RNA or a polypeptide of interest using a chimeric gene of the invention.