Abstract: Provided is an engineered nuclease with high salt tolerance. The polypeptide comprises one or more mutations so that the polypeptide possesses more positive charged surface area in its three-dimensional structure. The polypeptide has at least 60% nuclease activity under a solution ionic strength more than 200 mM as compared to the nuclease having the sequence of SEQ ID NO: 1.

Abstract: A system and method for providing a tunable floating-point multiply-accumulate (MAC) unit are disclosed. The unit maintains full arithmetic precision while enabling dynamic elimination of ineffectual computation through operand decomposition and selective activation of partial product generation logic. The disclosed MAC unit is suitable for drop-in replacement in existing deep-learning accelerators and improves energy efficiency without requiring architectural changes.

Abstract: Disclosed is a method for collecting bacteria from bacterial or fungal fermentation culture broth, the method comprising the steps of: a) mixing bacterial or fungal fermentation culture medium with a carboxylated chitosan to obtain a mixture; optionally, b) adding a sodium alginate solution to the mixture and mixing homogeneously; and c) precipitating to obtain bacterial or fungal cell precipitate or culture supernatant.

Abstract: The present disclosure relates to a system for the extraction of macromolecules comprising: one or more incubators, the incubators for culturing a biological sample to provide a base sample for screening for the detection of the macromolecule; a sample operating system, the sample operating system for performing predetermined operations on the biological sample cultured in the incubator; a sample detection platform, the sample detection platform for detecting the biological sample for screening; and a transfer platform, the transfer platform for transferring the biological samples between the incubator and the sample operating system and/or between the sample operating system and the sample detection platform. The system according to the present disclosure not only quantitatively increases the throughput of extraction of macromolecules while quantitatively reducing the number of operators, but also greatly improves the stability, homogeneity, and reproducibility of the process by standardizing operations.

Abstract: The present invention relates inter alia to methods of biosynthetic production of QS-21, precursors and variants thereof, and to related aspects.

Abstract: An intelligent system for gene synthesis and monoclonal picking, comprising: a core operation module for performing operations associated with gene synthesis and monoclonal picking; and a control system for controlling at least a part of operations of corresponding compartments of the core operation module. The core operation module at least includes: a liquid operation compartment for performing operations of mixing gene fragments and mixing synthetizing reagents with biological samples in the process of gene synthesis; a purification compartment for performing purification operations on nucleic acid products created after the mixing; an amplification compartment for performing amplification operations and error correction operations on fragments of the nucleic acid products and performing culturing and amplification operations on vectors; and a monoclonal picking compartment for performing monoclonal picking operations on purified and cultured nucleic acid products.

Abstract: The disclosure generally relates to the fields of immunology, cell biology, molecular biology and medicine. More particularly, it concerns polypeptide complex for conjugation and use thereof. A polypeptide complex comprising, from N-terminus to C-terminus, a Fab domain operably linked to a hinge region is provided, wherein the Fab domain and the hinge region are derived from different IgG isotypes, or part thereof. The polypeptide complex further comprises a Fc polypeptide which is operably linked to the hinge region. The present disclosure provides an antibody drug conjugate comprising a polypeptide complex of the present disclosure.

Abstract: The present invention provides, inter alia, methods for treating or ameliorating the effects of a cancer in a subject, e.g., glioblastoma (GBM) in a subject that is resistant to temozolomide (TMZ), methods for delaying the emergence of TMZ resistance and/or increasing the TMZ sensitivity in a subject, and methods for reducing tumor aggressiveness in a subject. Also provided are pharmaceutical compositions and kits to implement such methods.

Abstract: A sensor housing system is used to monitor the lifetime of a motor or other device. A protected housing shields a microprocessor and several sensors. The device communicates to a user information about the health of the motor or other device using thermal, vibrational, or other measurements. The information is compared to a baseline which provides a warning threshold. Once the threshold is passed, the microprocessor can alert the user that the motor or other device is about to fail.

Abstract: A sensor housing system is used to monitor the lifetime of a motor or other device. A protected housing shields a microprocessor and several sensors. The device communicates to a user information about the health of the motor or other device using thermal, vibrational, or other measurements. The information is compared to a baseline which provides a warning threshold. Once the threshold is passed, the microprocessor can alert the user that the motor or other device is about to fail.