Abstract: The present disclosure provides a class of Pyridopyrimidine compounds having a structure shown in Formula (I) or their pharmaceutically acceptable salts, or stereoisomers or prodrug molecules and applications thereof. The compounds in the present disclosure can efficiently and selectively degrade AKT3 protein in cells without affecting AKT1/2, thereby significantly inhibiting tumor cell proliferation mediated by high expression of AKT3 protein. It can be used to prepare therapeutic drugs for cancer and other diseases related to abnormal expression of AKT3 protein.

Abstract: The inventive technology is directed to droplet compositions having novel endoskeletal and/or exoskeletal shell architectures configured to produce enhanced vaporization characteristics.

Abstract: The invention relates to a microfluidic poration device having narrow channels slightly smaller than the width of a target cell, wherein the channels are lined with a plurality of nanospikes in a row extending down the middle of the channel, i.e. in a row parallel to the sides of the channel. In one embodiment, one channel may have 2 nanospikes (or 2 nanolancets). Thus, in particular embodiments, the invention provides microfluidic poration devices capable of simultaneously squeezing cells while piercing holes in their membranes for allowing foreign molecules into cells. The holes in porated cells spontaneously close after exiting the channels, thus entrapping the foreign molecules inside of the target cells. This porated cell population has approximately a 95% viability with greater than 50% containing at least one foreign molecule.

Abstract: The invention relates to a microfluidic poration device having narrow channels slightly smaller than the width of a target cell, wherein the channels are lined with a plurality of nanospikes in a row extending down the middle of the channel, i.e. in a row parallel to the sides of the channel. In one embodiment, one channel may have 2 nanospikes (or 2 nanolancets). Thus, in particular embodiments, the invention provides microfluidic poration devices capable of simultaneously squeezing cells while piercing holes in their membranes for allowing foreign molecules into cells. The holes in porated cells spontaneously close after exiting the channels, thus entrapping the foreign molecules inside of the target cells. This porated cell population has approximately a 95% viability with greater than 50% containing at least one foreign molecule.

Abstract: A new on chip system, named Acoustic Gym, to apply a surface acoustic wave (SAW) has been developed to control the swimming exercise of a large number of small animals such as C. elegans and Zebra fish, at desired intensities and durations. The SAW device consists of a circular chamber filled with fluid, which is placed in the center between a pair of offset interdigital transducers (IDTs) that are deposited on a piezoelectric substrate. Upon actuation from a radio frequency (RF) source, the SAW is generated and propagates along the substrate towards the microchamber. Once the SAW reaches the chamber, it induces an acoustic streaming in the fluid inside the chamber to stimulate swimming exercise of small animals. The streaming velocity can be precisely controlled via the RF signal input power, thus regulating the duration and intensity of exercise of the animals.

Abstract: Thermal shift assays (TSAs) have been extensively used to study thermodynamics of proteins and provide an efficient means to assess protein-ligand binding or protein-protein interaction. However, existing TSAs have limitations such as time consuming, labor intensive, or low sensitivity. Here, an acousto-thermal shift assay (A-TSA) is disclosed and is believed to be the first ultrasound enabled TSA which can provide a real-time analysis of protein thermodynamic stability. A-TSA couples unique acoustic mechanisms to achieve protein unfolding, concentration, and measurement on a single microfluidic chip within minutes. Compared to conventional TSA methods, A-TSA provides an ultra-fast (at least 30 times faster), highly sensitive (7-34 folds higher), and label -free monitoring of protein-ligand interactions and protein stability finding applications for protein analysis in biology, medicine and fast diagnosis.

Abstract: The invention relates to a microfluidic poration device having narrow channels slightly smaller than the width of a target cell, wherein the channels are lined with a plurality of nanospikes in a row extending down the middle of the channel, i.e. in a row parallel to the sides of the channel. In one embodiment, one channel may have 2 nanospikes (or 2 nanolancets). Thus, in particular embodiments, the invention provides microfluidic poration devices capable of simultaneously squeezing cells while piercing holes in their membranes for allowing foreign molecules into cells. The holes in porated cells spontaneously close after exiting the channels, thus entrapping the foreign molecules inside of the target cells. This porated cell population has approximately a 95% viability with greater than 50% containing at least one foreign molecule.

Abstract: A microfluidic system for causing perturbations in a cell membrane includes (a) a microfluidic channel defining a lumen and configured such that a cell suspended in a buffer can pass there through, and (b) source or emitter of an energy field. The microfluidic channel may include a cell-deforming constriction. A diameter of the constriction may be a function of the diameter of the cell. Related apparatus, systems, techniques, and articles are also described.

Abstract: Methods and apparatus that facilitate membrane-perturbing surface interactions for delivering a payload to a variety of cell types without resulting in a substantial loss in cell viability or alteration of endogenous cellular functions. In one example, an intracellular delivery tool comprises a microfluidic device (10) which includes a microfluidic flow channel (12) containing fluid therein and a membrane perturbing surface (22), in fluid communication with the microfluidic flow channel (12), with a plurality of perturbing features disposed thereon. An exemplary intracellular delivery method includes flowing a fluid containing cells therein along a membrane perturbing surface having a plurality of perturbing features disposed thereon, and delivering nanomaterial across a membrane of the cells in the fluid during and after contact between the cells and the membrane perturbing surface.

Abstract: A microfluidic system for causing perturbations in a cell membrane includes (a) a microfluidic channel defining a lumen and configured such that a cell suspended in a buffer can pass there through, and (b) source or emitter of an energy field. The microfluidic channel may include a cell-deforming constriction. A diameter of the constriction may be a function of the diameter of the cell. Related apparatus, systems, techniques, and articles are also described.

Abstract: An apparatus for sorting cells from a mixed population of cells using surface acoustic waves is described. Methods for separating cancer cells from a mixed population of cells are provided. Methods for separating cells or particles having different size, density and/or compressibility properties are also provided.

Abstract: An apparatus for manipulating particles within a fluid sample includes a substrate having a substrate surface. A surface acoustic wave (SAW) generator generates a SAW within a SAW region of the substrate surface. The SAW has an SAW direction aligned with a pressure node. A channel is configured to receive the fluid sample and the fluid sample has a flow direction which is at an oblique angle to the SAW direction.

Abstract: An apparatus for manipulating particles uses tunable standing surface acoustic waves includes a channel defined on a substrate and a pair of variable frequency interdigital transducers. The channel is disposed asymmetrically between the transducers such that the zero order node location is outside of a working region in the channel.

Abstract: An apparatus for manipulating particles within a fluid sample includes a substrate having a substrate surface. A surface acoustic wave (SAW) generator generates a SAW within a SAW region of the substrate surface. The SAW has an SAW direction aligned with a pressure node. A channel is configured to receive the fluid sample and the fluid sample has a flow direction which is at an oblique angle to the SAW direction.

Abstract: A two-dimensional barcode includes a plurality of macro modules arranged in a two-dimensional array in a spiral sequence, each of macro modules includes a predetermined number of data unit modules for storing data codewords of a predetermined binary stream of bits, a predetermined number of cluster unit modules for storing a predetermined cluster number representing a location of the macro module, and a predetermined number of rim unit modules which possess a predetermined color to form a rim of the macro module. The rim unit modules of two adjacent macro modules are contrasting with each other to enhance identification of the respective macro module.

Abstract: The present invention relates to a scanning device with a positioning senor and a processing method to obtain images, comprising a casing, linear contact image sensor (CIS), further comprising a system processor P3 for controlling the work of said CIS by timing signal, the optical coordinate sensors and the displacement processor P1 and P2 for estimating the coordinate increment (8001) and the coordinate increment (8002) of the real-time displacement, an A/D circuit and data buffer; said system processor P3 packets original scanning data with positioning information (8004), and then transfers to the upper machine.

Abstract: A two-dimensional barcode includes a plurality of macro modules arranged in a two-dimensional array in a spiral sequence, each of macro modules includes a predetermined number of data unit modules for storing data codewords of a predetermined binary stream of bits, a predetermined number of cluster unit modules for storing a predetermined cluster number representing a location of the macro module, and a predetermined number of rim unit modules which possess a predetermined color to form a rim of the macro module. The rim unit modules of two adjacent macro modules are contrasting with each other to enhance identification of the respective macro module.

Abstract: A two-dimensional barcode includes a plurality of macro modules arranged in a two-dimensional array in a spiral sequence, each of macro modules includes a predetermined number of data unit modules for storing data codewords of a predetermined binary stream of bits, a predetermined number of cluster unit modules for storing a predetermined cluster number representing a location of the macro module, and a predetermined number of rim unit modules which possess a predetermined color to form a rim of the macro module. The rim unit modules of two adjacent macro modules are contrasting with each other to enhance identification of the respective macro module.

Abstract: The present invention relates to an information card with multiple storage media. Combining reading and writing characteristics of magnetic strip storages, ICs, PFIDs, and etc. with large storage capacity of a compound printing 2D barcode, the invention uses a base card with a magnetic strip storage medium or a base card beset with an IC read-write chip, and a RF read-write chip. On said base card contains a first 2D barcode which has difference reflectivities to lights in the first spectrum area between bars and spaces, there is a designated relation between data stored in the magnetic strip or the IC chip, RF chip and data stored in the first 2D barcode. The invention discloses a read-write device of the information card comprising a Contact Image Sensor (CIS), a magnetic head or an IC chip reader, an RF chip reader. The invention further discloses a method for reading and writing data in said information card.

Abstract: An insert-type barcode reader has a body support with an insertion slot for receiving a card with barcode. A circuit board and a CIS component module electrically connected to the circuit board with each mounted to the body support to scan and process signals. A roller is mounted to the body support for movement relative to the CIS component module to allow the card to be properly guided into the insertion slot and tightly pressed onto the CIS component for accurate scanning of the barcode on the card. Two photoelectric sensors mounted to the body support are electrically connected to the circuit board for sensing the card entering and leaving the insertion slot to control starting, running and stopping of the scanning function of the CIS component module.