Abstract: A biological sample processing device includes a containing bottle. A receiving opening is provided and a liquid outlet is provided. The device further includes an upper cover assembly and a lower cover. The upper cover assembly is connected to the upper end of the containing bottle. The lower cover is connected to the lower end of the containing bottle. The upper cover assembly includes an upper cover, a lower seal, a piston and a puncturing element. The upper cover is provided with a through hole, and the lower seal is disposed at a lower end of the through hole. The piston is closely matched with an inner wall of the through hole and can slide along the inner wall to drive the puncturing element to pierce the lower seal. The disclosure provides advantages that the biological sample can be processed by the provided device without opening the upper cover.