Abstract: The present disclosure disclosed recombinase aided amplification (RAA) primers and kits for the detection of hepatitis c virus. Nucleotide sequences of the RAA primers include: an upstream primer: 5?-FITC-CTTGGGATATGATGATGAACTGGTCACCTAC-3? (SEQ ID NO. 1); and a downstream primer: 5?-Biotin-AAGAGTAGCATCACAATCAGAACCTTAGCC-3? (SEQ ID NO. 2). The HCV detection by using the RAA primers screened by the present disclosure has good specificity and high sensitivity (at least 10 copies/?L can be detected). The RAA primers can be used to prepare an HCV detection kit and construct an RAA amplification system. And combined with a lateral flow chromatography technology, the present disclosure can achieve rapid and low-cost detection of HCV and visual result judgment, no complex professional background is required, the use process is convenient and fast, and the detection results are safe and reliable.