Abstract: The present disclosure belongs to the technical field of biology, and in particular relates to an attenuated strain of African swine fever virus (ASFV) with IPTG-induced deletion of a D1133L gene and use thereof. In the present disclosure, it is firstly found that the D1133L protein of ASFV can inhibit production of IFN-? and downstream cytokines ISG-15 and ISG-56, and can be used as an immunosuppressant with a relatively strong immunosuppressive effect. In addition, the attenuated strain of ASFV with IPTG-induced deletion of the D1133L gene is constructed. Specifically, a screening expression cassette is inserted into a position before the non-structural protein gene D1133L of the ASFV using an Escherichia coli lac operator-repressor system, to obtain a recombinant virus. In the presence of the IPTG, the recombinant virus has similar characteristics to a wild-type virus; and in the absence of the IPTG, the expression of the D1133L protein is inhibited.

Abstract: A method for disassembling and separating a waste lithium-ion battery comprises: directly tearing a battery pack or a cell of the waste lithium-ion battery with water and electricity without discharging after removing a housing, then performing first wet screening, directly performing wet degumming without drying after recovering an electrolyte and removing iron by magnetic separation, then performing first crushing with water, third wet screening and second crushing with water after performing second wet screening, and finally performing jigging separation to obtain copper powder, aluminum powder, positive and negative electrode materials, plastic powder and separator pulp.

Abstract: A manganese-lithium separation process and a pre-extraction solution preparation process in comprehensive recovery of ternary battery wastes, and a method for comprehensive recovery of cobalt, nickel, manganese and lithium elements from the ternary battery wastes, relates to a method for recycling battery wastes. According to the present disclosure, cobalt and nickel ions are separated from an impurity-removed solution by a hydrolysis method; manganese, lithium and other ions in the impurity-removed solution are free from an extraction procedure, so that most manganese ions are separated and removed by a wet method before extraction, to prevent the manganese ions from entering the extraction system; nickel ions are free from an extraction procedure of full extraction and full back-extraction; and nickel hydroxide is directly precipitated after related impurities are removed by extraction.

Abstract: The present disclosure provides a method for preparing lithium carbonate from lithium iron phosphate battery scraps and lithium carbonate thereof, and relates to a method for preparing lithium carbonate from lithium iron phosphate battery scraps and a lithium carbonate product thereof. The method specifically includes the following steps; performing oxidative acidolysis on a lithium iron phosphate material, thickening lithium solution, adding alkali to remove iron, and precipitating lithium carbonate, thereby obtaining a filter cake which is a lithium carbonate product. Lithium may be directly extracted by utilizing the lithium iron phosphate battery scraps. A technical problem in the prior art that lithium iron phosphate battery wastes cannot be effectively decomposed and recycled is solved. The method has the characteristics of simple process, high, lithium yield, and low production cost.

Abstract: A method for preparing nickel/manganese/lithium/cobalt sulfate and tricobalt tetraoxide from battery wastes adopts the following process: dissolving battery wastes with acid, removing iron and aluminum, removing calcium, magnesium and copper, carrying extraction separation, and carrying out evaporative crystallization to prepare nickel sulfate, manganese sulfate, lithium sulfate, cobalt sulfate or/and tricobalt tetraoxide. By using the method, multiple metal elements, such as nickel, manganese, lithium and cobalt, can be simultaneously recovered from the battery wastes, the recovered products are high in purity and can reach battery grade, battery-grade tricobalt tetraoxide can also be directly produced. The method is simple in process, low in energy consumption and free in exhaust gas pollution, and can realize zero release of wastewater.

Abstract: A method for preparing industrial grade lithium carbonate from crude lithium fluoride includes the following steps: a, pulping by stirring crude lithium fluoride into a pulp, and adding an acid to prepare a crude lithium fluoride pulp-like material; b, double decomposition by adding the lithium fluoride pulp-like material obtained in the step a into a boiling calcium chloride solution, and then adding an alkaline substance to obtain a lithium chloride solution; c, lithium carbonate precipitation by heating the lithium chloride solution obtained in the step b, adding a carbonate solution according to the mass of lithium in the lithium chloride solution, stirring at a constant temperature, and filtering, wherein the filter cake is a lithium carbonate product. The lithium fluoride is decomposed at one time in a low-acidity environment; fluoride ions are removed; and a double decomposition reaction is used to decompose the lithium fluoride into lithium ions and calcium fluoride precipitates.

Abstract: A method for recovering lithium from low-content extraction tailwater and recycling extraction tailwater is provided. The disclosure is characterized that recovery of lithium from lithium-containing extraction tailwater is achieved by adding calcium to remove fluorine, carrying out evaporative crystallization and precipitating lithium salts. Recycle of extraction tailwater is achieved by adopting the following steps: in the lithium-containing extraction tailwater, adding calcium to remove fluorine, carrying out evaporative crystallization, recovering condensate water, precipitating a lithium salt and recycling mother liquor. According to the disclosure, lithium is recovered from low-content extraction tailwater via enrichment and sodium sulfate and distilled water therein are incidentally recovered, so that zero release of battery waste treatment wastewater is achieved.

Abstract: The present disclosure provides a method for preparing lithium carbonate from lithium iron phosphate battery scraps and lithium carbonate thereof, and relates to a method for preparing lithium carbonate from lithium iron phosphate battery scraps and a lithium carbonate product thereof. The method specifically includes the following steps; performing oxidative acidolysis on a lithium iron phosphate material, thickening lithium solution, adding alkali to remove iron, and precipitating lithium carbonate, thereby obtaining a filter cake which is a lithium carbonate product. Lithium may be directly extracted by utilizing the lithium iron phosphate battery scraps. A technical problem in the prior art that lithium iron phosphate battery wastes cannot be effectively decomposed and recycled is solved. The method has the characteristics of simple process, high, lithium yield, and low production cost.

Abstract: A method for preparing nickel/manganese/lithium/cobalt sulfate and tricobalt tetraoxide from battery wastes adopts the following process: dissolving battery wastes with acid, removing iron and aluminum, removing calcium, magnesium and copper, carrying extraction separation, and carrying out evaporative crystallization to prepare nickel sulfate, manganese sulfate, lithium sulfate, cobalt sulfate or/and tricobalt tetraoxide. By using the method, multiple metal elements, such as nickel, manganese, lithium and cobalt, can be simultaneously recovered from the battery wastes, the recovered products are high in purity and can reach battery grade, battery-grade tricobalt tetraoxide can also be directly produced. The method is simple in process, low in, energy consumption and free in exhaust gas pollution, and can realize zero release of wastewater.

Abstract: A method for recovering lithium from low-content extraction tailwater and recycling extraction tailwater is provided. The disclosure is characterized that recovery of lithium from lithium-containing extraction tailwater is achieved by adding calcium to remove fluorine, carrying out evaporative crystallization and precipitating lithium salts. Recycle of extraction tailwater is achieved by adopting the following steps: in the lithium-containing extraction tailwater, adding calcium to remove fluorine, carrying out evaporative crystallization, recovering condensate water, precipitating a lithium salt and recycling mother liquor. According to the disclosure, lithium is recovered from low-content extraction tailwater via enrichment and sodium sulfate and distilled water therein are incidentally recovered, so that zero release of battery waste treatment wastewater is achieved.

Abstract: A method for preparing industrial grade lithium carbonate from crude lithium fluoride includes the following steps: a, pulping by stirring crude lithium fluoride into a pulp, and adding an acid to prepare a crude lithium fluoride pulp-like material; b, double decomposition by adding the lithium fluoride pulp-like material obtained in the step a into a boiling calcium chloride solution, and then adding an alkaline substance to obtain a lithium chloride solution; c, lithium carbonate precipitation by heating the lithium chloride solution obtained in the step b, adding a carbonate solution according to the mass of lithium in the lithium chloride solution, stirring at a constant temperature, and filtering, wherein the filter cake is a lithium carbonate product. The lithium fluoride is decomposed at one time in a low-acidity environment; fluoride ions are removed; and a double decomposition reaction is used to decompose the lithium fluoride into lithium ions and calcium fluoride precipitates.