Patents by Inventor Ximiao WEN
Ximiao WEN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240102000Abstract: Provided herein is a method of making, comprising transporting reagents to a reactor in a microfluidic path device, wherein the reagents include a synthetic gene of interest, a polymerase, a buffer, a first primer having a first region specific to the synthetic gene of interest, and a second primer, wherein the second primer comprises a poly-T sequence of ?150 base pairs (bp) or a poly-A sequence of ?150 bp and a second region specific to the synthetic gene of interest; controlling a temperature of the first reactor to perform a polymerase chain reaction within the microfluidic path device to amplify the synthetic gene of interest using the first primer and the second primer to form a synthetic product including the poly-A sequence of ?150 bp; and transporting the synthetic product out of the first reactor, wherein the synthetic product comprises a synthetic DNA template for in vitro transcription.Type: ApplicationFiled: February 8, 2022Publication date: March 28, 2024Inventors: Samuel Deutsch, Sangeeta Nath, Ximiao Wen, Wei Cai, Babak Sanii, Benjamin Eldridge
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Patent number: 11926817Abstract: Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.Type: GrantFiled: August 10, 2020Date of Patent: March 12, 2024Assignee: NUTCRACKER THERAPEUTICS, INC.Inventors: Benjamin Eldridge, Ximiao Wen
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Publication number: 20240018506Abstract: Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.Type: ApplicationFiled: June 29, 2023Publication date: January 18, 2024Inventors: Benjamin Eldridge, Ximiao Wen
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Publication number: 20240018507Abstract: Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.Type: ApplicationFiled: June 29, 2023Publication date: January 18, 2024Inventors: Benjamin Eldridge, Ximiao Wen
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Publication number: 20230356221Abstract: The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.Type: ApplicationFiled: May 19, 2023Publication date: November 9, 2023Inventors: Benjamin Eldridge, Ximiao Wen
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Patent number: 11724257Abstract: The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.Type: GrantFiled: April 25, 2022Date of Patent: August 15, 2023Assignee: NUTCRACKER THERAPEUTICS, INC.Inventors: Benjamin Eldridge, Ximiao Wen
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Patent number: 11571693Abstract: The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.Type: GrantFiled: February 28, 2022Date of Patent: February 7, 2023Assignee: NUTCRACKER THERAPEUTICS, INC.Inventors: Benjamin Eldridge, Ximiao Wen
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Publication number: 20230035784Abstract: An apparatus includes a process chip and a dynamic light scattering assembly. The process chip includes a fluid chamber including and an optically transmissive material adjacent to the fluid chamber. The process chip is to be removably positioned in relation to the dynamic light scattering assembly. The dynamic light scattering assembly is to direct the light through the optically transmissive material and into the fluid chamber. The dynamic light scattering assembly is further to receive light scattered by particles in fluid in the fluid chamber in response to the first optical fiber emitting light into the fluid chamber and thereby capture light scattering data. A processor determines viscosity of fluid in the fluid chamber based on the captured light scattering data. The processor also determines one or both of size or size distribution of particles in the fluid based the captured light scattering data.Type: ApplicationFiled: October 12, 2022Publication date: February 2, 2023Inventors: Benjamin Eldridge, Ximiao Wen, Eric Chu, Tamas Czimmerman, Kenneth Jordan, Jodi Loo, Babak Sanii
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Publication number: 20220323953Abstract: The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.Type: ApplicationFiled: April 25, 2022Publication date: October 13, 2022Inventors: Benjamin Eldridge, Ximiao Wen
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Publication number: 20220250058Abstract: The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.Type: ApplicationFiled: February 28, 2022Publication date: August 11, 2022Inventors: Benjamin Eldridge, Ximiao Wen
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Patent number: 11325122Abstract: The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.Type: GrantFiled: June 28, 2021Date of Patent: May 10, 2022Assignee: NUTCRACKER THERAPEUTICS, INC.Inventors: Benjamin Eldridge, Ximiao Wen
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Patent number: 11278895Abstract: The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.Type: GrantFiled: June 28, 2021Date of Patent: March 22, 2022Assignee: NUTCRACKER THERAPEUTICS, INC.Inventors: Benjamin Eldridge, Ximiao Wen
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Publication number: 20210322938Abstract: The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.Type: ApplicationFiled: June 28, 2021Publication date: October 21, 2021Inventors: Benjamin Eldridge, Ximiao Wen
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Publication number: 20210322975Abstract: The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.Type: ApplicationFiled: June 28, 2021Publication date: October 21, 2021Inventors: Benjamin Eldridge, Ximiao Wen
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Publication number: 20210040472Abstract: Methods and apparatuses for making and using therapeutics, including in particular mRNA therapeutics, that separate double-stranded RNA from single-stranded RNA as part of a continuous flow. These methods and apparatuses may include formulation of an RNA therapeutic using a permeable insert integrated into a microfluidic path device. In particular, these methods and apparatuses may include formulation of an RNA therapeutic by removing dsRNA from a solution of RNA by within a microfluidic path device including a cellulose material.Type: ApplicationFiled: August 10, 2020Publication date: February 11, 2021Inventors: Samuel DEUTSCH, Benjamin ELDRIDGE, Colin McKINLAY, Ximiao WEN, Sangeeta NATH, Igor KHANDROS, Robert EVANS
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Publication number: 20210039106Abstract: Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.Type: ApplicationFiled: August 10, 2020Publication date: February 11, 2021Inventors: Benjamin ELDRIDGE, Ximiao WEN