Abstract: A medical adhesive and the preparation method, and application thereof. The medical adhesive is in the form of a gel, which contains a giant salamander skin mucus and an aqueous solution; the ratio parts by weight of the giant salamander skin dried powder and the aqueous solution is 1:1 to 1:6, and the weight content of the giant salamander skin mucus freeze-dried powder in the medical adhesive ranges from 14.2% to 50%. The present invention also provides the preparation method for preparing the aforementioned medical adhesive, and at the same time, the aforementioned medical adhesive is applied for the wound.