Abstract: The present disclosure provides a preparation and application of high-activity and high-safety Cardamine violifolia selenoprotein standard sample. The standard sample of the Cardamine violifolia selenoprotein prepared by the present disclosure has the advantages of extremely low heavy metal content, less ash, high protein content, high total selenium content, high organic selenium ratio of up to 99.9%, high biological activity, high safety and so on; the organic selenium is mainly in form of selenocysteine/selenocystine and accounts for more than 90% of the total selenium content, and is expected to make up for the shortage of selenoprotein standard samples on the market.

Abstract: A method, system, and composition related to the preparation of samples for glycoproteomic analysis is described. The sample preparation process can include a proteolytic digestion step followed by a measurement step of the glycopeptide and peptide amounts in the proteolytic digest using a liquid chromatography-mass spectrometry system. Optionally, the sample preparation process can also include the collection of the sample on an absorbent or bibulous member where the proteins and glycoproteins are later extracted and then digested for glycoproteomic analysis. Glycopeptide and peptide measurements of biological samples were analyzed to provide a diagnosis of a disease such as, for example, ovarian cancer or to assess whether a patient with melanoma is likely or not likely to benefit from checkpoint inhibitor therapy.

Abstract: Embodiments disclosed herein generally relate to technologies for evaluating a biological sample obtained from a subject with respect to a sepsis state or coronavirus disease (COVID). Some methods relating to the technologies can include receiving peptide structure data corresponding to the biological sample obtained from the subject, identifying a peptide structure profile for the biological sample using the peptide structure data, and computing a disease indicator using the peptide structure profile and a model. The disease indicator can indicate whether the biological sample is positive for the sepsis state. The disease indicator can indicate whether the biological sample is positive for COVID. The peptide structure profile can comprise quantification data for a set of peptide structures associated with the sepsis state. The peptide structure profile can include peptides that are glycosylated, aglycosylated, or both.

Abstract: The present disclosure provides a preparation and application of high-activity and high-safety Cardamine violifolia selenoprotein standard sample. The standard sample of the Cardamine violifolia selenoprotein prepared by the present disclosure has the advantages of extremely low heavy metal content, less ash, high protein content, high total selenium content, high organic selenium ratio of up to 99.9%, high biological activity, high safety and so on; the organic selenium is mainly in form of selenocysteine/selenocystine and accounts for more than 90% of the total selenium content, and is expected to make up for the shortage of selenoprotein standard samples on the market.