Abstract: The present application relates to a nano-liter photoionization mass spectrometry ion source device and an operation method thereof. The nano-liter photoionization mass spectrometry ion source device includes a nano-tip, configured to load a sample solution, thus achieving a nano-electrospray process; a metal electrode, inserted into the nano-tip to contact with the sample solution directly, thus providing a high-voltage electric field for the nano-electrospray; and a UV lamp, configured to emit a high-energy ultraviolet photon to be combined with a gaseous molecule obtained by vaporizing the sample solution, thus achieving a photoionization process. Directed to the problems such as low ionization efficiency, poor sensitivity and more impurity interference existing in the unicellular mass spectrometry process of trace low-polar compounds in small-volume samples, a nano-liter photoionization mass spectrometry ion source device suitable for the analysis on low-polar compounds in small volume, e.g.

Abstract: The present disclosure provides an artificial cell for single-cell mass spectrometry (SCMS) measurement and a preparation method thereof. In the present disclosure, the artificial cell includes an internal aqueous phase, an intermediate oil phase, and an external aqueous phase; where the internal aqueous phase includes polyethylene glycol (PEG) and a polyvinyl alcohol (PVA) aqueous solution, and the intermediate oil phase includes a chloroform-hexane mixture of L-?-phosphatidylcholine; and the external aqueous phase includes PVA and an F-68 aqueous solution. Compared with natural cell samples, new artificial single cells based on microfluidic self-assembly provided by the present disclosure have better uniformity, stability, and controllability. The artificial cell can effectively avoid significant measurement differences between individual single-cell samples, and effectively solve the problem of difficulty in stably preserving biological samples.