Abstract: A multidimensional liquid chromatography separation system has a mobile-phase storage tank, a first liquid transfer device, a second liquid transfer device, a first sample introduction device, a second sample introduction device, a separation device, a collection, storage device, at least two drainage devices and a flow path switching device. A separation method for protein separation using the multidimensional liquid chromatography separation system has the steps of: 1) preparation in advance, 2) the first dimensional separation, 3) collection and storage of the intermediate fraction, 4) the second dimensional or multidimensional separation and repeating the steps 3) and 4).

Abstract: A multidimensional liquid chromatography separation system has a mobile-phase storage tank, a first liquid transfer device, a second liquid transfer device, a first sample introduction device, a second sample introduction device, a separation device, a collection, storage device, at least two drainage devices and a flow path switching device. A separation method for protein separation using the multidimensional liquid chromatography separation system has the steps of: 1) preparation in advance, 2) the first dimensional separation, 3) collection and storage of the intermediate fraction, 4) the second dimensional or multidimensional separation and repeating the steps 3) and 4).

Abstract: The present invention discloses a new and improved kind of chromatographic cake and its manufacturing methods and applications. The objective of this invention is to provide an improved chromatographic apparatus and methods for manufacturing such an apparatus, whereby the separation and simultaneous renaturation and purification of biopolymers can be efficiently performed with excellent results. The chromatographic cakes of this invention generally comprise a chromatographic packing cake with a mobile phase inlet and a mobile phase outlet, together with a chromatographic packing packed into an inner cavity region of the chromatographic packing cake. The ratio of the thickness to the diameter of the inner cavity region of the chromatographic packing cake is less than or equal to 1.

Abstract: The present invention discloses a new and improved kind of chromatographic cake and its manufacturing methods and applications. The objective of this invention is to provide an improved chromatographic apparatus and methods for manufacturing such an apparatus, whereby the separation and simultaneous renaturation and purification of biopolymers can be efficiently performed with excellent results. The chromatographic cakes of this invention generally comprise a chromatographic packing cake with a mobile phase inlet and a mobile phase outlet, together with a chromatographic packing packed into an inner cavity region of the chromatographic packing cake. The ratio of the thickness to the diameter of the inner cavity region of the chromatographic packing cake is less than or equal to 1.