Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO.1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

Abstract: A heave survey platform includes: a body, two fixed wings and two variable wing mechanisms. The two fixed wings are symmetrically disposed on two sides of a middle part of the body, and an axis of the body is located in a plane defined by extension directions of the two fixed wings. Each variable wing mechanism includes a variable wing, and the two variable wings of the two variable wing mechanisms are symmetrically disposed on two sides of a lower end of the body, and each variable wing is configured to swing between a first position coplanar with the plane and a second position forming an angle with the plane to generate a lift force on the body at the second position, thereby to make the body move in a radial direction or change attitude.

Abstract: A driving method is implemented to a lifting device of an underwater survey system, and the lifting device includes a phase-change heat exchange module, an oil bag module, a pressurized energy storage module, and a drive energy storage module. The driving method includes: controlling the pressurized energy storage module to extract hydraulic oil in the oil bag module to descend the lifting device; during a transformation of a phase-change material, the pressurized energy storage module transmitting hydraulic oil to the phase-change heat exchange module; transmitting the hydraulic oil inside the oil bag module to the pressurized energy storage module to descend the lifting device; controlling the drive energy storage module to transmit hydraulic oil to the oil bag module for rising the lifting device; and during a transformation of the phase-change material, transmitting the hydraulic oil in the phase-change heat exchange module to the drive energy storage module.

Abstract: A driving method is implemented to a lifting device of an underwater survey system, and the lifting device includes a phase-change heat exchange module, an oil bag module, a pressurized energy storage module, and a drive energy storage module. The driving method includes: controlling the pressurized energy storage module to extract hydraulic oil in the oil bag module to descend the lifting device; during a transformation of a phase-change material, the pressurized energy storage module transmitting hydraulic oil to the phase-change heat exchange module; transmitting the hydraulic oil inside the oil bag module to the pressurized energy storage module to descend the lifting device; controlling the drive energy storage module to transmit hydraulic oil to the oil bag module for rising the lifting device; and during a transformation of the phase-change material, transmitting the hydraulic oil in the phase-change heat exchange module to the drive energy storage module.

Abstract: Disclosed is a method for purifying pea albumin PAla by using a negatively charged polysaccharide, which belongs to the field of protein extraction technology. According to the invention, with a natural edible negatively charged polysaccharide as a recycling material, based on the technical principle that the negatively charged polysaccharide preferentially binds to non-target proteins to precipitate and undergoes phase separation relative to the target protein, the separation and purification of pea albumin PAla are realized to prepare pure PAla. From the perspective of resource recycling, the invention can realize high value-added utilization of byproducts of pea protein isolate processing and prepare high-purity high-sulfur active protein components therefrom, thereby providing technical support for subsequent research.

Abstract: The invention relates to a plant protein isolate containing less than 10 microgram, preferably less than 5 microgram, of the sum of hexanal, 2-pentyl-furan, (E)-2,4,heptadienal and 1-octen-3-ol per gram of dry matter and its method of preparation. The plant protein is preferably obtained from leguminous plant, more preferably from pea or fava bean, most preferably from pea. The method for extracting the plant protein isolate consists of the steps: (a) providing a protein containing seed, (b) milling said seed, (c) suspending the milled seed in water, (d) extracting proteins from said milled suspension and (e) washing the extracted proteins with water at a temperature between 60° C. and 100° C. and at a pH in the range of 4 to 5.5.