Abstract: The present disclosure provides a preparation method of a broad bean protein and starch-based foamed hydrogel, and a use thereof in preparation of a vibration-damping packaging. The preparation method includes the following steps: stirring a broad bean protein solution with a concentration of 40 g/L to 60 g/L until foaming; under stirring, adding a sodium alginate-starch mixed solution in a volume twice a volume of the broad bean protein solution, followed by stirring until homogeneous; and adding calcium carbonate until a final concentration of the calcium carbonate reaches 10 g/L to 12 g/L, and further stirring thoroughly to produce the broad bean protein and starch-based foamed hydrogel, where in the sodium alginate-starch mixed solution, a concentration of sodium alginate is 22.5 g/L to 27.5 g/L, and a concentration of corn starch is 120 g/L to 160 g/L.

Abstract: A method for measuring water exchange across the blood-brain barrier includes acquiring diffusion weighted (DW) arterial spin labeling (ASL) magnetic resonance imaging (MRI) signals. The method further includes determining optimal parameters to separate labeled water in capillary and brain tissue compartments. The method further includes estimating water exchange rate across the blood-brain barrier based on the DW ASL MRI signals and the optimal parameters, using a total generalized variation (TGV) regularized single-pass approximation (SPA) modeling algorithm.

Abstract: The present invention discloses a Prunus persica polygalacturonase-inhibiting protein 1 (PpPGIP1) gene, and a cloning method and use thereof. The Prunus persica polygalacturonase-inhibiting protein 1 PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by the Prunus persica polygalacturonase-inhibiting protein 1 (PpPGIP1) gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product.

Abstract: The present invention discloses a peach polygalacturonase-inhibiting protein PpPGIP1 gene, and a cloning method and use thereof. The peach polygalacturonase-inhibiting protein PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by the peach polygalacturonase-inhibiting protein PpPGIP1 gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product. As there is a protein-protein interaction relationship between PpPGIP1 and PpVIN2, the effective inhibition of the PpPGIP1 expression in peach can significantly reduce the activity of the acid invertase PpVIN2 and thus reduce the decomposition of sucrose.

Abstract: A method for measuring water exchange across the blood-brain barrier includes acquiring diffusion weighted (DW) arterial spin labeling (ASL) magnetic resonance imaging (Mill) signals. The method further includes determining optimal parameters to separate labeled water in capillary and brain tissue compartments. The method further includes estimating water exchange rate across the blood-brain barrier based on the DW ASL MRI signals and the optimal parameters, using a total generalized variation (TGV) regularized single-pass approximation (SPA) modeling algorithm.