Abstract: A method for extracting and identifying a polysaccharide from a complex sample. The method includes: subjecting a complex sample to be treated to sterilization and enzyme deactivation to obtain a system, and subjecting the system to solid-liquid separation to obtain a sterilization and enzyme deactivation-treated solution; deproteinizing the sterilization and enzyme deactivation-treated solution to obtain a deproteinized solution; purifying the deproteinized solution by using a hydrophilic lipophilic balance-solid phase extraction (HLB-SPE) cartridge to obtain a purified solution; subjecting the purified solution to alcohol precipitation to obtain the polysaccharide; and subjecting the polysaccharide to methylation, glycoside residue derivatization and liquid chromatography-mass spectrometry (LC-MS) analysis in sequence.

Abstract: Provided are a food additive for dispersing a Ganoderma lucidum powder and a method for rapidly dispersing a Ganoderma lucidum powder. The food additive for dispersing a Ganoderma lucidum powder includes a plant polyphenol and a metal-derived food additive in a mass ratio of (50-2,500):(0.1-0.2). The method for rapidly dispersing a Ganoderma lucidum powder with the food additive includes: mixing an aqueous solution of the plant polyphenol with the Ganoderma lucidum powder to obtain a Ganoderma lucidum/polyphenol mixed solution; and thoroughly mixing an aqueous solution of the metal-derived food additive with the Ganoderma lucidum/polyphenol mixed solution.

Abstract: The present disclosure provides use of an intestinal probiotic in preparation of a medicament that promotes metabolism of ellagic acid (EA) into urolithin A, a pharmaceutical composition and use thereof, and belongs to the technical field of biomedicine. In the present disclosure, probiotics capable of changing metabotypes of EA are selected from a plurality of probiotics. These probiotics alone or in combination can change an intestinal flora structure of a metabotype B and/or 0 population, thereby improving the efficiency of the metabolism of EA into urolithin A and increasing a yield of urolithin A. The present disclosure provides an intestinal probiotic for involving in and promoting the metabolism of EA into urolithin A, and provides a new idea for the treatment of diabetes, obesity, senescence, and diseases caused by central nervous system lesions and viral infection using urolithin A as an active pharmaceutical ingredient.

Abstract: The present disclosure provides a water-soluble dietary fiber (WSDF), use thereof in preparation of a medicament that promotes metabolism of ellagic acid (EA) into urolithin A, and a pharmaceutical composition, and belongs to the technical field of biological pharmaceuticals. The WSDF provided by the present disclosure includes at least two components selected from the group consisting of gellan gum, guar gum, carrageenan, fructooligosaccharide, xylooligosaccharide, malto-oligosaccharide, pectin, ?-glucan, polydextrose, sodium alginate, gum arabic, resistant dextrin, xanthan gum, tragacanth gum, and inulin. In the present disclosure, compounding the foregoing different combinations of the WSDF and EA to feed mice may significantly increase the average urolithin A content in mouse feces.

Abstract: The present disclosure discloses a preparation method of porous starch for encapsulating probiotic, and belongs to the field of food processing. The present disclosure provides porous starch with controllable pore size and morphology by regulating the ratio of amylose to amylopectin (quantitative compounding), keeping starch hydroxyl sites exposed (concentration cultivation), and building interfacial tension to stretch starch chains (convection drying), and uses the porous starch to encapsulate probiotics, which can improve the retention rate of probiotics, reduce the loss of probiotics during food processing and transportation, and thus retain the biological functions of probiotics to a maximum extent.

Abstract: A preparation method for a nanopore starch-based adsorbent. The method mainly comprises: constructing a large number of lamellar crystals on surfaces of starch granules, and inducing the formation of a nano-scale pore channel structure by means of the lamellar crystals. A large number of control tests have proved that the flaky crystals have direct influence on the formation of the nano-scale pore channel structure. The construction of a pore channel structure in a starch matrix material greatly increases the specific surface area, and improves the adsorption properties. In addition, the method is beneficial to the introduction of functional particles (magnetic particles), and avoids the problem of pore channel blockage due to first forming pores and then introducing functional particles.

Abstract: The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 ?L of NaOH-DMSO suspension (1 mg/mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 ?L of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.

Abstract: The disclosure aims to provide a chemical synthesis method of a prodelphinidin B9 gallate, in which the prodelphinidin B9 gallate is synthesized by using a proanthocyanidin from Chinese bayberry leaves as a raw material, and using epigallocatechin gallate (EGCG) as a nucleophilic reagent to attack C4 sites of subunits EGCG and epigallocatechin (EGC) of the proanthocyanidin from Chinese bayberry leaves in the presence of hydrochloric acid as a catalyst. Compared with prodelphinidin B9 gallates extracted and separated from materials such as tea leaves, and Chinese bayberry leaves, the prodelphinidin B9 gallate prepared by the method provided in the present disclosure has higher purity and yield, and can be directly used as a nutrient enhancer and a natural antioxidant in the field of food.

Abstract: The present invention discloses an ultrasound-electrode-nano-porous membrane coupling hydrogen production and sterilization system, which comprises a vessel and a plurality of hydrogen production units arranged in the vessel. The hydrogen production unit comprises a cavity, and a broadband ultrasonic generator and a circular hydrogen production electrode located in the cavity. The circular hydrogen production electrode wraps around the broadband ultrasonic generator. A bottom membrane at the bottom of the cavity is hydrophobic at the inner side and hydrophilic at the outer side, and a side membrane of the cavity is hydrophilic at the inner side and hydrophobic at the outer side and is mounted vertically or obliquely upwards by an angle of 0-45 degrees.

Abstract: The present disclosure discloses a method for extracting pectin rich in RG-I, in particular to an ultrasound and pressure assisted method for extracting pectin rich in RG-I. The main process includes: dispersing sieved citrus peel powers in alkaline solution containing sodium borohydride (20 to 50 mM); performing an ultrasound and pressure combined treatment on the above solution at 25 to 40° C., the treatment conditions include: static pressure of 0.2 to 0.4 MPa, ultrasonic intensity of 0.5 to 3.5 W/mL, and treatment time of 20 to 40 min; centrifuging the ultrasonically processed solution to remove residues, and sequentially carrying out precipitation with ethanol (50% to 80% v/v), precipitant dialysis (3000 to 3500 Da), and vacuum freeze drying on the extracted solution to obtain pectic polysaccharide.

Abstract: Provided are a lycium barbarum leaf polysaccharide rich in galacturonic acid and a preparation method and use thereof. The method includes: mixing a lycium barbarum leaf and an acetone aqueous solution to obtain a mixture, and performing a fading treatment on the mixture to obtain a faded lycium barbarum leaf; extracting the faded lycium barbarum leaf with a chelating agent solution to obtain an extract solution; subjecting the extract solution to an alcohol precipitation with ethanol to obtain an alcohol precipitate; and subjecting the alcohol precipitate to an alcohol washing, a water redissolution, a dialysis and a drying in sequence to obtain the lycium barbarum leaf polysaccharide rich in galacturonic acid.

Abstract: The present disclosure belongs to the technical field of food processing and provides a phenolic stabilized fruit juice and a preparation method thereof. In the present disclosure, plant RG-I pectin polysaccharide is extracted through an ultra-high pressure technology, and then ultra-high pressure technology is used to promote the interaction of pectin polysaccharides and phenols in fruit juice, improve the stability of phenolic substances in fruit juice, and can improve the bioavailability of phenolic substances in fruit juice. The method of the present disclosure improves the added value of products and solves the problem of low bioavailability of phenolic substances in juice.

Abstract: The present disclosure discloses an ultrasonic-assisted method of extracting a pectin rich in RG-I. The method includes: (1) dispersing citrus peel powders in an alkaline solution containing sodium borohydride; (2) placing a solution obtained in step (1) in a water bath of 25 to 40° C., and performing an ultrasonic treatment on the solution obtained in step (1); (3) centrifuging the solution subjected to step (2) at a speed of 6000-8000 rpm to remove residues, retaining a supernatant, and precipitating a pectic polysaccharide with absolute ethanol; and (4) adding water to the pectic polysaccharide precipitant subjected to step (3) to obtain a redissolved solution, transferring the solution to a dialysis bag with a molecular weight cut-off of 3000 to 3500 Da, dialyzing for 48 hours, and freeze-drying in a vacuum freezer to obtain the pectin rich in RG-I.

Abstract: An extraction method of proanthocyanidins from Myrica rubra Sieb. Et Zucc leaves is provided. In the method, proanthocyanidins from Myrica rubra Sieb. Et Zucc leaf are obtained by preparing an aqueous Myrica rubra Sieb. Et Zucc leaf suspension and centrifuging. In the method, only water is used as an extraction reagent without introduction of an organic extraction solvent, which reduces costs and is free from environmental pollution; moreover, the method does not cause organic reagent residue in a product, such that the biological activity of the proanthocyanidins is not affected and product safety is improved without affecting product quality; meanwhile, extraction steps are simplified, extraction time is reduced and extraction efficiency is improved. The method provides stability, good reproducibility, simple equipment and easy control of conditions.

Abstract: A preparation method for a nanopore starch-based adsorbent. The method mainly comprises: constructing a large number of lamellar crystals on surfaces of starch granules, and inducing the formation of a nano-scale pore channel structure by means of the lamellar crystals. A large number of control tests have proved that the flaky crystals have direct influence on the formation of the nano-scale pore channel structure. The construction of a pore channel structure in a starch matrix material greatly increases the specific surface area, and improves the adsorption properties. In addition, the method is beneficial to the introduction of functional particles (magnetic particles), and avoids the problem of pore channel blockage due to first forming pores and then introducing functional particles.

Abstract: The present disclosure discloses a method for extracting pectin rich in RG-I, in particular to an ultrasound and pressure assisted method for extracting pectin rich in RG-I. The main process includes: dispersing sieved citrus peel powers in alkaline solution containing sodium borohydride (20 to 50 mM); performing an ultrasound and pressure combined treatment on the above solution at 25 to 40° C., the treatment conditions include: static pressure of 0.2 to 0.4 MPa, ultrasonic intensity of 0.5 to 3.5 W/mL, and treatment time of 20 to 40 min; centrifuging the ultrasonically processed solution to remove residues, and sequentially carrying out precipitation with ethanol (50% to 80% v/v), precipitant dialysis (3000 to 3500 Da), and vacuum freeze drying on the extracted solution to obtain pectic polysaccharide.

Abstract: The present disclosure discloses an ultrasonic-assisted method of extracting a pectin rich in RG-I. The method includes: (1) dispersing citrus peel powders in an alkaline solution containing sodium borohydride; (2) placing a solution obtained in step (1) in a water bath of 25 to 40° C., and performing an ultrasonic treatment on the solution obtained in step (1); (3) centrifuging the solution subjected to step (2) at a speed of 6000-8000 rpm to remove residues, retaining a supernatant, and precipitating a pectic polysaccharide with absolute ethanol; and (4) adding water to the pectic polysaccharide precipitant subjected to step (3) to obtain a redissolved solution, transferring the solution to a dialysis bag with a molecular weight cut-off of 3000 to 3500 Da, dialyzing for 48 hours, and freeze-drying in a vacuum freezer to obtain the pectin rich in RG-I.

Abstract: The present disclosure discloses a method for extracting a RG-I-rich pectic polysaccharide. The method comprises the following steps: mixing and stirring a citrus fruit peel powder and a dilute hydrochloric acid solution at a low temperature to destroy cell walls of citrus peels, filtering, and then mixing the retentate with a sodium hydroxide solution for low temperature extraction, adding ethanol to the solution obtained by filtering to precipitate, and washing the obtained polysaccharide with ethanol and drying to obtain the above pectic polysaccharide. Compared with the traditional extraction method, this method reduces the hydrolysis of pectin side chains by controlling the extraction temperature, and the extracted pectic polysaccharide is rich in RG-I domain and has higher biological activity such as cancer and cardiovascular disease prevention. Energy can be greatly saved because of the low extraction temperature.

Abstract: Disclosed is a method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning, including the following steps: adding fresh citrus fruit peels to alkali/acid processing water from citrus fruit canning for extracting; filtering the resulting mixture, adding to the resulting filtrate 95% ethanol 1 to 3 times the volume of the filtrate, and then adjusting the pH to a range of 3.5 to 7, followed by standing for 10 minutes to 4 hours; filtering the product after standing, washing the resulting precipitate with 50% to 70% ethanol, and drying and crushing, thereby obtaining pectin. The method makes full use of waste resources from citrus fruit canning, solves the problem of pollution by processing discharge water, and has the advantages of saving the preparation cost of pectin from citrus peels, and improving the solubility of recovered pectin.

Abstract: The present invention discloses a mixture of fucosylated chondroitin sulfate oligosaccharides having an average molecular weight between 2700 Da and 3600 Da, in which 90% of total components has a molecular weight between 1800 Da and 5400 Da. The mixture of fucosylated chondroitin sulfate oligosaccharide of the present invention has very low anti-FIIa factor activity, very low anti-FXa factor activity, very high anti-FXase factor activity, i.e., having a significant activity in selectively inhibiting intrinsic FXase, and a significant antithrombotic activity and no risk of bleeding and hypotension that are proved through animal experiments. The present invention also discloses a method for rapidly producing fucosylated chondroitin sulfate oligosaccharides by using sea cucumber as raw material, and the method includes: intercepting and concentrating crude polysaccharide with a membrane, deacetylation with hydrazine, pyrolysis with nitrous acid, reduction with sodium borohydride, membrane separation, etc.