Abstract: The present disclosure provides a preparation method of a mesenchymal stem cell (MSC) and an application thereof in a knee product. The additive composition including NOG and quercetin provided by the present disclosure includes components that are combined scientifically and reasonably and play a synergistic role, and has a stable efficacy.

Abstract: The present disclosure provides an in vitro method for preparation of human pluripotent stem cells (HPSCs) from human adipocyte-derived stem cells (ADSCs) without any genetic engineering techniques and without involving any exogenous gene elements, plasmid or transcription factors and the so obtained HPSCs are referred to as directly-generated human pluripotent stem cells (dgHPSCs). The present invention further provides an in vitro method for insulin production from the dgHPSCs by means of single- or co-transduction with human estrogen-related receptor gamma (ERR?) gene by the lentivirus vector pWPI/ERR? encoding the human ERR? gene and/or with human insulin (INS) gene by a lentivirus vector, pWPI/INS encoding the human INS gene, where the insulin secreted by such co-transduced cells is higher than singly transduced cells.

Abstract: Method for preparing and administering human pluripotent stem cells includes preparing human adipose stem cells from a human; epigenetically modifying the human adipose stem cells (hADSC) to yield directly-generated human pluripotent stem cells (dgHPSC); and engineering the dgHPSC to secrete a therapeutic level of insulin. Preparing the human adipose stem cells includes obtaining a lipoaspirate from a human and preparing adipose stem cells from the lipoaspirate. Epigenetically modifying the hADSC includes inducing the hADSC to yield the dgHPSC. Engineering the dgHPSC includes transducing a human estrogen-related receptor gamma (ERR?) gene into the dgHPSC. Engineering the dgHPSC further includes transducing a human INS gene into the dgHPSC. The dgHPSC transduced with the ERR? gene and the human INS gene secrete a higher level of insulin compared to the dgHPSC transduced with the ERR? gene. The engineered dgHPDC are introduced into a human.