Abstract: A portable sample loading device (1,4,7) include a base (11,41,71) and a fixing device (11,41,71) connected to the base (12,42,72). The base (12,42,72) can support a chip (2,5) and a sample loading connector (3,6). The fixing device (12,42,72) can detachably retain the chip (2,5) and the sample loading connector (3,6) on the base (11,41,71). The base (11,41,71) includes a front surface (110,410,710) and a back surface (111,411,711) opposite to the front surface (110,410,710). The front surface (110,410,710) includes a supporting surface for supporting the chip (2,5). A region of the supporting surface (112,412,712) corresponds to a sample inlet of the chip (2,5) is recessed toward the back surface (111,411,711) to form a receiving space (114,117,414,714) for receiving the sample loading connector (3,6). The receiving space (114,117,414,714) further defines a sample injection hole (115, 415,715) corresponding to the sample inlet of the chip (2,5).