Abstract: A joint apparatus and a vehicle, where the joint apparatus includes a first joint, a second joint and a limiting assembly, where the first joint includes a first extension portion and a second extension portion, which are provided in a first direction, and an end of the second joint is provided with a socket. When the first joint and the second joint are in a mounted state, the first extension portion is inserted into the socket in the first direction, and the limiting assembly is configured to limit the first joint and the second joint from moving away from each other in the first direction.

Abstract: A joint apparatus and a vehicle, where the joint apparatus includes a first joint, a second joint and a limiting assembly, where the first joint includes a first extension portion and a second extension portion, which are provided in a first direction, and an end of the second joint is provided with a socket. When the first joint and the second joint are in a mounted state, the first extension portion is inserted into the socket in the first direction, and the limiting assembly is configured to limit the first joint and the second joint from moving away from each other in the first direction.

Abstract: A crude oil transportation drag-reducing agent testing loop test bench and its use method is provided. The test bench includes a gas supply and pressurization system, a pressurized storage tank system, a pipeline testing system, a reflux and circulation system, a valve switching system, a heating and insulation testing system, and a sensor testing system. The test bench can evaluate the performance of drag-reducing agents in transportation pipeline devices within a laboratory setting, and further inspect, evaluate, and test the effective performance of different proportions and types of drag-reducing agents. The test bench also ensures the safety of experimental test, reduces testing costs, avoids the production of large amounts of waste liquid, and minimizes test space.

Abstract: An in-situ observation device for gas hydrates includes a reactor, which includes a visual tube, a pulling rod, a temperature-controlled seed crystal rod and a spiral adjuster. An upper end and a lower end of the visual tube are respectively equipped with an upper end cap and a lower end cap. The upper end cap defines an air inlet, and the lower end cap defines a liquid inlet. An upper end of the temperature-controlled seed crystal rod is connected to an adjusting flange. The adjusting flange is connected to the spiral adjuster. A lower end of the temperature-controlled seed crystal rod hermetically passes through the upper end cap and is located in the visual tube. The temperature-controlled seed crystal rod defines a cooling liquid channel, and a cooling liquid inlet and a cooling liquid outlet, a seed crystal rod liquid inlet and a seed crystal rod outlet.

Abstract: A low-frequency acoustic wave generating device for detecting pipeline blockage related to the technical field of pipeline detection is provided and includes a sealing shell configured to be communicated with a pipeline, a sealed pressure environment is formed inside the sealing shell. A low-frequency electroacoustic transducer is disposed inside the sealing shell, the low-frequency electroacoustic transducer is connected to a signal generator and is configured to transmit an acoustic wave into the pipeline. The device transmits the acoustic wave signals to a high-pressure gas pipeline, combined with a signal generator capable of generating any waveform, thereby emitting any waveform of acoustic wave signal into the pipeline. The use of acoustic waves with special markings and characteristics for pipeline blockage detection can improve the anti-noise interference ability of the acoustic wave detection, and improve the precision of the detection.

Abstract: Implementations of this specification provide prediction methods and apparatuses for adjusting computing power. One method comprises receiving a prediction request, wherein the prediction request comprises a sample to be tested, determining a computing power coefficient allocated to the prediction request, wherein the computing power coefficient indicates a proportion of hardware computing power resources allocated to the prediction request to total hardware computing power resources needed for a neural network model to run on a computing platform, determining k sub-networks inn sub-networks of the neural network model to be used for a present time based on the computing power coefficient, where n>2, and inputting the sample to be tested to the k sub-networks to obtain a prediction result.

Abstract: A multi-booth traffic allocation scheme for multiple users if provided. In an implementation, an exposure probability of each booth and a unit traffic revenue and corresponding unit resource consumption of each candidate display object for each user are obtained. An optimization objective and a resource consumption constraint corresponding to a multi-booth traffic allocation scheme are constructed. The constructed optimization objective includes a sum value of traffic revenues for each user under the multi-booth traffic allocation scheme. The traffic revenues for each user are determined based on the exposure probability of each booth, a booth allocation scheme of candidate display objects, and the unit traffic revenue of each candidate display object. A multi-booth traffic allocation scheme when the resource consumption constraint is satisfied and the optimization objective is maximized is determined.

Abstract: The present invention provides a method for genomic profiling of DNA 5-methylcytosine and 5-hydroxymethylcytosine, comprising the following steps: (1) DNA purification and fragmentation pretreatment: the target DNA is extracted and then broken to an average of 50 nucleotides to 10,000 nucleotides in length; (2) the repair of trace amount of DNA and the ligation thereof to the adaptor: the pre-treated DNA fragments are repaired and ligated with the sequencing adaptor required for the second-generation sequencing, (3) covalently labeling 5-methylcytosine and 5-hydroxymethylcytosine, (4) solid-phase enrichment of the labeled DNA fragments having cytosine with 5-position modification; (5) the PCR amplification of the solid-phase enriched DNA fragments, the PCR product is obtained and purified to obtain a library for the second-generation sequencing, after mapping the sequencing reads to the genome, the distribution map of the cytosine with 5-position modification in the DNA sample could be generated.

Abstract: The current disclosure provides a method that can specifically label and directly amplify 5hmC site on genomic DNA without pull-down or bisulfite treatment, which enables one to map the 5hmC site from a single DNA molecule. Aspects of the disclosure relate to a method for detecting 5-hydroxymethylcytosine (5hmC) nucleic acid bases in a nucleic acid molecule or a plurality of nucleic acid molecules, the method comprising: a. modifying the 5hmC nucleic acid base with a first functional group; b. covalently attaching a modified nucleic acid probe comprising a second functional group to the first functional group; wherein the nucleic acid probe and nucleic acid molecule are covalently linked through the first and second functional groups; c. annealing a primer to the nucleic acid probe; d. performing primer extension of the annealed primer to make a new strand; and e. detecting the new strand.

Abstract: Disclosed is a high-throughput sequencing method for methylated CpG island in trace DNA, comprising the steps of: 1) treating a DNA sample with bisulfite; 2) adding the treated sample obtained in step 1) to a PCR system containing a primer A for linear amplification; 3) adding an exonuclease having single-stranded DNA cleaving activity to the PCR product in step 2) and inactivating the exonuclease after the reaction; 4) adding the product in step 3) to a PCR system containing a primer B for linear amplification; 5) adding the product in step 4) to a PCR system containing the corresponding adapter primer C and adapter primer D for amplification; and 6) purifying the PCR product in step 5) to obtain a DNA library with a specific length and carrying out the sequencing.

Abstract: Disclosed is a high-throughput sequencing method for methylated CpG island in trace DNA, comprising the steps of: 1) treating a DNA sample with bisulfite; 2) adding the treated sample obtained in step 1) to a PCR system containing a primer A for linear amplification; 3) adding an exonuclease having single-stranded DNA cleaving activity to the PCR product in step 2) and inactivating the exonuclease after the reaction; 4) adding the product in step 3) to a PCR system containing a primer B for linear amplification; 5) adding the product in step 4) to a PCR system containing the corresponding adapter primer C and adapter primer D for amplification; and 6) purifying the PCR product in step 5) to obtain a DNA library with a specific length and carrying out the sequencing.

Abstract: The present invention provides a method for genomic profiling of DNA 5-methylcytosine and 5-hydroxymethylcytosine, comprising the following steps: (1) DNA purification and fragmentation pretreatment: the target DNA is extracted and then broken to an average of 50 nucleotides to 10,000 nucleotides in length; (2) the repair of trace amount of DNA and the ligation thereof to the adaptor: the pre-treated DNA fragments are repaired and ligated with the sequencing adaptor required for the second-generation sequencing, (3) covalently labeling 5-methylcytosine and 5-hydroxymethylcytosine, (4) solid-phase enrichment of the labeled DNA fragments having cytosine with 5-position modification; (5) the PCR amplification of the solid-phase enriched DNA fragments, the PCR product is obtained and purified to obtain a library for the second-generation sequencing, after mapping the sequencing reads to the genome, the distribution map of the cytosine with 5-position modification in the DNA sample could be generated.

Abstract: A method for identifying a 5-MeC in a template nucleic is provided. The method comprises providing a template having 5-MeC, converting the 5-MeC into a further modification selected from 5-caC and 5-FC. The converted template is then sequenced, and a change in sequencing is detected that is indicative of the further modification, allowing for identifying the 5-MeC in the template nucleic acid.

Abstract: The present invention relates to methods and compositions for detecting, evaluating, and/or mapping 5-methyl-modified and/or 5-hydroxylmethyl-modified cytosine bases within a nucleic acid molecule.

Abstract: A method for identifying a 5-MeC in a template nucleic is provided. The method comprises providing a template having 5-MeC, converting the 5-MeC into a futher modification selected from 5-caC and 5-FC. The converted template is then sequenced, and a change in sequencing is detected that is indicative of the further modification, allowing for identifying the 5-MeC in the template nucleic acid.