Abstract: The invention encompasses microfluidic microarray assemblies (MMA) and subassemblies and methods for their manufacture and use. In one embodiment, first and second channel plates are provided and are sealingly connected to a test chip in consecutive steps. Each plate includes microfluidic channels configured in a predetermined reagent distribution pattern. The test chip comprises a plurality of discrete test positions, each test position being located at the intersection between a first predetermined reagent pattern and a second predetermined reagent pattern, wherein at least one of said patterns is non-linear. The first channel plate allows the distribution of a first reagent on said test chip, wherein said first reagent is immobilized at said test positions. The second channel plate allows the distribution of a second reagent on said test chip, wherein said second reagent comprises a plurality of different test samples.

Abstract: The invention relates to a microfluidic device comprising one or more fluid channels, one or more fluid ports, and a V-shaped particle retention structure. The fluid channel is generally opposite the particle retention structure, fluid ports are located between the fluid channel and the particle retention structure, and the particle retention structure has sloped side walls. Fluid, including reagents, can be delivered to the microfluidic device through the one or more fluid channels or the fluid ports. The invention also relates to methods of using the microfluidic device to monitor, observe, measure, or record a biological parameter of a particle, to separate a single particle from a group of particles, to culture a cell, to treat a particle, and to move a particle back and forth in the device.

Abstract: Microarray devices fabricated using microfluidic reagent distribution techniques are provided. As described herein, the invention encompasses microfluidic microarray assemblies (MMA) and subassemblies and methods for their manufacture and use. In one embodiment first and second channel plates are provided which may be sealed to a test chip in consecutive steps. Each channel plate includes microfluidic channels configured in a predetermined reagent distribution pattern. For example, the first channel plate may have a radial (linear) reagent distribution pattern and the second channel plate may have a spiral (curved) reagent distribution pattern, or vice versa. In one embodiment the first channel plate is connected to the test chip and at least one first reagent is distributed on the test chip in a first predetermined reagent pattern. The first reagent is then immobilized on the test chip.

Abstract: The invention relates to a microfluidic device comprising one or more fluid channels, one or more fluid ports, and a V-shaped particle retention structure. The fluid channel is generally opposite the particle retention structure, fluid ports are located between the fluid channel and the particle retention structure, and the particle retention structure has sloped side walls. Fluid, including reagents, can be delivered to the microfluidic device through the one or more fluid channels or the fluid ports. The invention also relates to methods of using the microfluidic device to monitor, observe, measure, or record a biological parameter of a particle, to separate a single particle from a group of particles, to culture a cell, to treat a particle, and to move a particle back and forth in the device.