Abstract: The invention is directed to cell-targeted cytotoxic agents, including sortase serine protease constructs. Methods for targeted cell killing for treatment of proliferative diseases, for example, cancer, are provided. Exemplary embodiments comprise an R-spondin ligand for targeting the cytotoxic agents to effect the cell killing.

Abstract: The invention is directed to cell-targeted cytotoxic agents, including sortase serine protease constructs. Methods for targeted cell killing for treatment of proliferative diseases, for example, cancer, are provided. Exemplary embodiments comprise an R-spondin ligand for targeting the cytotoxic agents to effect the cell killing.

Abstract: Cell-targeted cytotoxic agents, including sortase serine protease constructs, are provided. Such compounds can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer). In some aspects, recombinant sortase serine proteases, such as Granzyme B polypeptides, are provided that exhibit improved stability and cell toxicity.

Abstract: The present disclosure provides a method for processing a live stream in web real-time communication (WebRTC) and a stream-pushing client. The method includes collecting an original live stream, and loading the original live stream into a temporary video tag, thereby playing the original live stream through the temporary video tag; creating a temporary image tag and loading a preset watermark image into the temporary image tag; creating a temporary drawing tag and drawing a current video frame in the temporary video tag and the preset watermark image in the temporary image tag into the temporary drawing tag; and acquiring a canvas video stream corresponding to the temporary drawing tag, and based on the canvas video stream and the original live stream, generating a watermark live stream with one or more added watermarks, wherein the watermark live stream is configured to be transmitted to a resource server through a WebRTC connection.

Abstract: A method and a stream-pushing client terminal for pushing audio and video based on a WebRTC protocol are provided. The method includes: calling, by a stream-pushing client terminal, an audio interface of a browser to create an audio processing module, and configuring, by the stream-pushing client terminal, a hybrid output unit on the audio processing module; inputting, by the stream-pushing client terminal, source data and additional data to the audio processing module, and generating, by the stream-pushing client terminal, processed data through the hybrid output unit; combining, by the stream-pushing client terminal, the source data and the processed data to generate hybrid sound data; and pushing, by the stream-pushing client terminal, the hybrid sound data to a resource server, to enable a stream-pulling client terminal to obtain the hybrid sound data from the resource server.

Abstract: The present disclosure describes a whiteboard and video synchronization method, apparatus, computing device and storage medium. The method includes that: a first terminal device acquires a video stream sent by a server, where each frame of video data in the video stream is provided with a timestamp, and the video stream is sent to the server by a second terminal device after the second terminal device collects each frame of video data and adds a timestamp to each frame of video data; the first terminal device then plays the video stream, and based on the timestamp of a currently played frame of the video stream, acquires a whiteboard content, corresponding to the timestamp of the currently played frame of the video stream, from the cache for synchronous play.

Abstract: The present invention is directed generally to cell-targeted cytotoxic constructs comprising a targeting polypeptide, a linking polypeptide and a cytotoxic polypeptide. Preferably, (a) the targeting polypeptide is a R-spondin1 (RSPO1), R-spondin2 (RSPO2) or yoked chorionic gonadotropin (YCG), the linking polypeptide comprises LPXT (SEQ ID NO: 56) or NPXT (SEQ ID NO: 60) as well as others, where X is any amino acid, the linking polypeptide being positioned between the targeting ligand and (c) the cytotoxic moiety is an auristatin or a truncated serine protease, the serine protease having an IIGG (SEQ ID NO: 91), IVGG (SEQ ID NO: 92) or ILGG (SEQ ID NO: 93) at its N-terminus. Such constructs can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer).

Abstract: Cell-targeted cytotoxic agents, including sortase serine protease constructs, are provided. Such compounds can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer). In some aspects, recombinant sortase serine proteases, such as Granzyme B polypeptides, are provided that exhibit improved stability and cell toxicity.

Abstract: The present invention features a novel cellular injury reporter system in which a chimeric gene containing the GADD153 promoter linked to the coding region of an enhanced green fluorescent protein (EGFP) gene was stably integrated into the genome of carcinoma cells. Activation of the GADD153 promoter was quantified using flow cytometric measurement of EGFP expression following drug exposure. This reporter system is suitable for high. throughput in vitro and in vivo screening for agents capable of producing cytotoxicity via a wide variety of different mechanisms, and can be utilized to investigate the relative potency of structurally related DNA adducts.

Abstract: The present invention features a novel cellular injury reporter system in which a chimeric gene containing the GADD153 promoter linked to the coding region of an enhanced green fluorescent protein (EGFP) gene was stably integrated into the genome of carcinoma cells. Activation of the GADD153 promoter was quantified using flow cytometric measurement of EGFP expression following drug exposure. This reporter system is suitable for high throughput in vitro and in vivo screening for agents capable of producing cytotoxicity via a wide variety of different mechanisms, and can be utilized to investigate the relative potency of structurally related DNA adducts.