Patents by Inventor XINLI EMILY WU
XINLI EMILY WU has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240002870Abstract: Methods for transforming monocot leaf explants are provided.Type: ApplicationFiled: September 28, 2021Publication date: January 4, 2024Applicants: PIONEER HI-BRED INTERNATIONAL, INC., CORTEVA AGRISCIENCE LLCInventors: AJITH ANAND, WILLIAM JAMES GORDON-KAMM, LARISA A. RYAN, NAGESH SARDESAI, NING WANG, HUIXIA WU, XINLI EMILY WU
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Publication number: 20230091338Abstract: Compositions and methods are provided for an inducible, high efficiency intra-genomic homologous recombination (IGHR) system in plants, that can be used for non-chimeric, heritable gene targeting. The advantage of IGHR approach to targeted integration is that every cell contains donor DNA and nuclease-encoding sequences, so that there are many potentially homology-directed targeting events that can occur during plant development.Type: ApplicationFiled: February 22, 2021Publication date: March 23, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L ARLING, PIERLUIGI BARONE, HYEON-JE CHO, WILLIAM JAMES GORDON-KAMM, SANDEEP KUMAR, BRIAN LENDERTS, KEITH S LOWE, SERGEI SVITASHEV, XINLI EMILY WU
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Patent number: 11330776Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: GrantFiled: August 26, 2016Date of Patent: May 17, 2022Inventors: Ajith Anand, Maren L. Arling, Alexandre Da Silva Conceicao, William James Gordon-Kamm, Craig E Hastings, George J Hoerster, Todd J Jones, Theodore Mitchell Klein, Carlos M La Rota, Keith S Lowe, Shiv Bahadur Tiwari, Ning Wang, Xinli Emily Wu
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Publication number: 20220124998Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: ApplicationFiled: January 7, 2022Publication date: April 28, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L ARLING, ALEXANDRE DA SILVA CONCEICAO, WILLIAM JAMES GORDON-KAMM, CRAIG HASTINGS, GEORGE J HOERSTER, TODD J JONES, THEODORE M KLEIN, CARLOS M LA ROTA, KEITH S LOWE, SHIV BAHADUR TIWARI, NING WANG, XINLI EMILY WU
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Patent number: 10278345Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: GrantFiled: August 26, 2016Date of Patent: May 7, 2019Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Randal Arnold, Roberto Barreiro, Matthew Paul Cope, Clifford P Hunter, Justin Andrew Schares, Xinli Emily Wu, Yue Yun
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Publication number: 20170273261Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: ApplicationFiled: July 7, 2015Publication date: September 28, 2017Inventors: Randal Arnold, Roberto Barreiro, Matthew Paul Cope, Clifford Paul Hunter, Justin Andrew Schares, Xinli Emily Wu, Yue Yun
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Publication number: 20170240911Abstract: Methods and compositions for targeting the insertion of a polynucleotide of interest into a specific chromosomal site in the genome of a plant cell using bacteria-mediated transformation methods are provided. The methods include providing a plant cell with at least one Transfer Cassette comprising in operable linkage a least one polynucleotide construct and at least one recombinase construct, and optionally at least one cell proliferation factor construct. Compositions provided include plant cells, plants or seeds having a polynucleotide of interest inserted at a specific chromosomal site.Type: ApplicationFiled: August 26, 2016Publication date: August 24, 2017Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, WILLIAM JAMES GORDON-KAMM, XINLI EMILY WU
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Patent number: 9706723Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: GrantFiled: July 7, 2015Date of Patent: July 18, 2017Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Randal Arnold, Roberto Barreiro, Matthew Paul Cope, Clifford P Hunter, Justin Andrew Schares, Xinli Emily Wu, Yue Yun
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Publication number: 20170121722Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: ApplicationFiled: August 26, 2016Publication date: May 4, 2017Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L. ARLING, ALEXANDRE DA SILVA CONCEICAO, WILLIAM JAMES GORDON-KAMM, CRAIG E. HASTINGS, GEORGE M. HOERSTER, THEODORE MITCHELL KLEIN, CARLOS M. LA ROTA, KEITH S. LOWE, SHIV BAHADUR TIWARI, NING WANG, XINLI EMILY WU
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Publication number: 20170006766Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: ApplicationFiled: July 7, 2015Publication date: January 12, 2017Inventors: RANDAL ARNOLD, Roberto Barreiro, Matthew Paul Cope, Clifford P Hunter, Justin Andrew Schares, Xinli Emily Wu, Yue Yun
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Publication number: 20160360716Abstract: Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.Type: ApplicationFiled: August 26, 2016Publication date: December 15, 2016Inventors: RANDAL ARNOLD, ROBERTO BARREIRO, MATTHEW PAUL COPE, CLIFFORD P. HUNTER, JUSTIN ANDREW SCHARES, XINLI EMILY WU, YUE YUN