Patents by Inventor Xin-Mei Zhang

Xin-Mei Zhang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for constructing and modifying large DNA molecules
    Patent number: 7781190
    Abstract: This invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule.
    Type: Grant
    Filed: July 16, 2004
    Date of Patent: August 24, 2010
    Assignee: The University of Hong Kong
    Inventors: Jian-Dong Huang, Xin-Mei Zhang, Julian Alexander Tanner
  • Method for constructing and modifying large DNA molecules
    Publication number: 20050084970
    Abstract: This invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule.
    Type: Application
    Filed: July 16, 2004
    Publication date: April 21, 2005
    Inventors: Jian-Dong Huang, Xin-Mei Zhang, Julian Tanner