Abstract: A pig myostatin gene locus and uses thereof are provided. Also provided includes an expression cassette comprising a promoter, a foreign gene and a following terminator; the promoter is a DNA molecule as set forth in any of 1)-4): 1) nucleotides at positions 2642-3778 starting from the 5? end of SEQ ID NO. 1 in the sequence listing; 2) nucleotides as set forth in SEQ ID NO. 1 in the sequence listing; 3) a DNA molecule, hybridizing and having the same function with the DNA sequence as defined in 1) or 2) under stringent condition. Experiments show that the pig myostatin gene locus provides a valuable gene source for gene targeting, as well as introducing and expressing a foreign gene at this site.

Abstract: A method for cultivating a transgenic animal with an increased expression amount of porcine growth hormone is provided. Additionally, a method for cultivating a transgenic embryo, which introduces a recombinant expression vector of 1) TRE-GH or 2) TET-ON into a target embryo to obtain a transgenic embryo with a higher expression amount of porcine growth hormone than a target embryo without introduction is provided. A transgenic animal can be obtained by transplanting the embryo into an animal. The experiments demonstrate that growth hormone (GH) is sharply increased in blood of transgenic pigs after adding DOX.