Patents by Inventor Xiomara L. MADERO
Xiomara L. MADERO has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11903708Abstract: A sensor implanted in tissues and including a sensing enzyme takes an electrical measurement and compares it to reference curves for the voltage current relationship. The sensor determines whether molecular compounds are present which interfere with the detection of the molecule of interest. If interfering species are found, the measurement voltage is set in a low range to reduce errors, while if the interfering species are not found, the measurement voltage is set in a high range to increase the detected signal.Type: GrantFiled: September 27, 2018Date of Patent: February 20, 2024Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Samson Chen, Axel Scherer, Muhammad Musab Jilani, Xiomara L. Madero
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Patent number: 11879162Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.Type: GrantFiled: July 5, 2022Date of Patent: January 23, 2024Assignee: California Institute of TechnologyInventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
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Publication number: 20230363672Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.Type: ApplicationFiled: May 17, 2023Publication date: November 16, 2023Inventors: Samson CHEN, Axel SCHERER, Dvin I. ADALIAN, Peter PETILLO, Muhammad Musab JILANI, Xiomara L. MADERO, Deepan KISHORE KUMAR
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Patent number: 11690544Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.Type: GrantFiled: May 7, 2021Date of Patent: July 4, 2023Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Samson Chen, Axel Scherer, Dvin Adalian, Peter Petillo, Muhammad Musab Jilani, Xiomara L. Madero, Deepan Kishore Kumar
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Publication number: 20230151440Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.Type: ApplicationFiled: July 5, 2022Publication date: May 18, 2023Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
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Patent number: 11414716Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.Type: GrantFiled: March 23, 2020Date of Patent: August 16, 2022Assignee: California Institute of TechnologyInventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
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Publication number: 20210321918Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.Type: ApplicationFiled: May 7, 2021Publication date: October 21, 2021Inventors: Samson CHEN, Axel SCHERER, Dvin ADALIAN, Peter PETILLO, Muhammad Musab JILANI, Xiomara L. MADERO, Deepan KISHORE KUMAR
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Patent number: 11026610Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.Type: GrantFiled: September 27, 2018Date of Patent: June 8, 2021Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Samson Chen, Axel Scherer, Dvin Adalian, Peter Petillo, Muhammad Musab Jilani, Xiomara L. Madero, Deepan Kishore Kumar
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Publication number: 20210002732Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.Type: ApplicationFiled: March 23, 2020Publication date: January 7, 2021Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
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Patent number: 10597737Abstract: Methods and kits for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. At least one of the primers is configured to hybridize to a region of the polynucleotide analyte encoding the genetic variation. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.Type: GrantFiled: August 22, 2018Date of Patent: March 24, 2020Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
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Publication number: 20190153548Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.Type: ApplicationFiled: August 22, 2018Publication date: May 23, 2019Inventors: ADITYA RAJAGOPAL, MARK D. GOLDBERG, ERIKA F. GARCIA, XIOMARA L. MADERO, THOMAS A. TOMBRELLO, AXEL SCHERER
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Patent number: 10081844Abstract: Methods of detecting at least one genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, the first primer and the second primer are specific for the polynucleotide analyte. A signal generated by the fluorophore and quencher is measured. PCR is performed with the first primer and the second primer using the polynucleotide analyte as a template, thereby amplifying the template. A signal generated by the fluorophore and quencher from the PCR amplification product is measured. Comparison is made of the signals; and a determination is made of the presence or absence of the at least one genetic variation based i) on the change in signal as determined; and ii) by comparing said change to the change in signal observed upon PCR amplification for a corresponding polynucleotide analyte lacking the at least one genetic variation.Type: GrantFiled: August 11, 2017Date of Patent: September 25, 2018Assignee: California Institute of TechnologyInventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
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Publication number: 20180030551Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.Type: ApplicationFiled: August 11, 2017Publication date: February 1, 2018Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
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Patent number: 9416343Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.Type: GrantFiled: November 1, 2013Date of Patent: August 16, 2016Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Imran R. Malik, Axel Scherer, Erika F. Garcia, Xiomara L. Madero
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Patent number: 8968585Abstract: Methods to fabricate reaction cartridges for biological sample preparation and analysis are disclosed. A cartridge may have a reaction chamber and openings to allow fluids to enter the chamber. The cartridge may also have handles to facilitate its use. Such cartridges may be used for polymerase chain reaction.Type: GrantFiled: November 1, 2013Date of Patent: March 3, 2015Assignee: California Institute of TechnologyInventors: Imran R. Malik, Axel Scherer, Erika F. Garcia, Xiomara L. Madero
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Patent number: 8873055Abstract: Structures and methods are described for optical detection of physical, chemical and/or biological samples. An optical detection structure may include a LED source, multiple filters and single or multiple sample areas. A detector may be used to record a fluorescence signal. The sample area may allow the introduction of removable cartridges.Type: GrantFiled: July 22, 2013Date of Patent: October 28, 2014Assignee: California Institute of TechnologyInventors: Imran R. Malik, Xiomara L. Madero, Erika F. Garcia, Axel Scherer
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Publication number: 20140213471Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.Type: ApplicationFiled: January 23, 2014Publication date: July 31, 2014Applicant: California Institute of TechnologyInventors: Aditya RAJAGOPAL, Mark D. GOLDBERG, Erika F. GARCIA, Xiomara L. MADERO, Thomas A. TOMBRELLO, Axel SCHERER
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Publication number: 20140127796Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.Type: ApplicationFiled: November 1, 2013Publication date: May 8, 2014Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Imran R. MALIK, Axel SCHERER, Erika F. GARCIA, Xiomara L. MADERO
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Publication number: 20140057210Abstract: Methods to fabricate reaction cartridges for biological sample preparation and analysis are disclosed. A cartridge may have a reaction chamber and openings to allow fluids to enter the chamber. The cartridge may also have handles to facilitate its use. Such cartridges may be used for polymerase chain reaction.Type: ApplicationFiled: November 1, 2013Publication date: February 27, 2014Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Imran R. MALIK, Axel SCHERER, Erika F. GARCIA, Xiomara L. MADERO
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Publication number: 20140036267Abstract: Structures and methods are described for optical detection of physical, chemical and/or biological samples. An optical detection structure may include a LED source, multiple filters and single or multiple sample areas. A detector may be used to record a fluorescence signal. The sample area may allow the introduction of removable cartridges.Type: ApplicationFiled: July 22, 2013Publication date: February 6, 2014Inventors: Imran R. MALIK, Xiomara L. MADERO, Erika F. GARCIA, Axel SCHERER