Abstract: Disclosed are methods, devices and kits for extracting, amplifying, detecting and visualizing nucleic acids of interest for use in onsite rapid diagnostics.

Abstract: Disclosed are compounds of Formula I, or a salt thereof: where A, B, D, X, R1, R2 and R8 are as defined herein, which compounds have properties for antagonizing PCSK9. Also described are pharmaceutical formulations comprising the compounds of Formula I or their salts, and methods of treating cardiovascular disease and conditions related to PCSK9 activity, e.g. atherosclerosis, hypercholesterolemia, coronary heart disease, metabolic syndrome, acute coronary syndrome, or related cardiovascular disease and cardiometabolic conditions.

Abstract: A prediction method for formation water yield of carbonate gas reservoir is provided, which includes: S1, constructing an initial prediction model for a formation water yield of a carbonate gas reservoir; S2, collecting and pre-processing raw data; where the raw data is raw data of parameters of the initial prediction model, and the raw data further includes raw data of a permeability when the initial prediction model does not involve permeability; S3, performing, according to the pre-processed raw data, an uncertainty analysis and a dimension reduction process on the parameters of the initial prediction model; S4, constructing a target prediction model for the formation water yield of the carbonate gas reservoir according to a result of dimension reduction process; and S5, obtaining, according to the target prediction model for formation water yield of carbonate reservoir, a predictive probability solution of formation water yield by combining a result of uncertainty analysis.

Abstract: Disclosed herein are “dual-priming” isothermal amplification method (including “self-priming” and “pairing-priming” strand extension, termed “DAMP”) for rapid nucleic acid detection.

Abstract: Provided is an analysis method for determining gas-bearing situation of an unknown shale reservoir, includes: S1, selecting a shale reservoir of a target interval of a place; and collecting data of parameters of cores of each of a known gas-bearing shale reservoir A and a known water-bearing shale reservoir B; S2, calculating average values of the parameters of each of the reservoirs A and B respectively; S3, calculating average differences of the parameters of each of the reservoirs; S4, calculating covariance values of the parameters of each of the reservoirs; S5, establishing, according to the covariance values, an equation group and resolving discriminant coefficients; S6, establishing a discriminant equation according to the discriminant coefficients and solving a discriminant index; and S7, obtaining values of parameters of cores of a sample of the unknown shale reservoir, calculating a discriminant value, and determining gas-bearing situation of the unknown shale reservoir.

Abstract: Provided is an analysis method for determining gas-bearing situation of an unknown shale reservoir, includes: S1, selecting a shale reservoir of a target interval of a place; and collecting data of parameters of cores of each of a known gas-bearing shale reservoir A and a known water-bearing shale reservoir B; S2, calculating average values of the parameters of each of the reservoirs A and B respectively; S3, calculating average differences of the parameters of each of the reservoirs; S4, calculating covariance values of the parameters of each of the reservoirs; S5, establishing, according to the covariance values, an equation group and resolving discriminant coefficients; S6, establishing a discriminant equation according to the discriminant coefficients and solving a discriminant index; and S7, obtaining values of parameters of cores of a sample of the unknown shale reservoir, calculating a discriminant value, and determining gas-bearing situation of the unknown shale reservoir.

Abstract: The disclosure provides methods and compositions for All-In-One Dual CRISPR-Cas12a (termed “AIOD-CRISPR”) permit simple, rapid, ultrasensitive, specific, one-pot and visual detection of nucleic acids (DNA and RNA).

Abstract: The disclosure provides materials and methods for detection of pathogens. In particular, the disclosure provides primers and compositions for detection of viral pathogens, such as SARS-CoV-2. In addition, the disclosure provides a warm-start digital CRISPR assay for detection of viral pathogens, including SARS-CoV-2.

Abstract: A method includes receiving an input specifying a recognition target. The method further includes selecting a plurality of models of an initial recognition layer based on the recognition target, and selecting a plurality of models of a final recognition layer based on the recognition target. The method includes obtaining sensor data from two or more sensors of a plurality of sensors, providing the sensor data to the plurality of models of the initial recognition layer to obtain an initial set of identifications, providing sensor data to the plurality of models of the final recognition layer to obtain a final set of identifications, and outputting an identification from at least one of the initial set of identifications or the final set of identifications.

Abstract: Disclosed herein are “dual-priming” isothermal amplification method (including “self-priming” and “pairing-priming” strand extension, termed “DAMP”) for rapid nucleic acid detection.