Abstract: An electroporation system including one or more of a pipette, a pipette tip, a pipette docking assembly, and a pulse generator. The pipette docking assembly includes a pipette station, a pipette station guard, and a reservoir (e.g., a buffer tube). A method for transfecting a cell with a payload including providing an electroporation system, providing the cell, providing the payload, introducing the cell and the payload into a pipette tip, and electroporating the cell within the pipette tip by operating the electroporation system.

Abstract: A protein transfer system includes at least one base configured to receive one or more consumable protein transfer stacks and at least one lid configured to cover the base. The lid(s) comprise(s) one or more electrodes for supplying current to the one or more consumable protein transfer stacks. The protein transfer system further includes at least one voltage source configured to supply the current to the one or more consumable protein transfer stacks, one or more processors, and one or more hardware storage devices storing instructions that are executable by the one or more processors to configure the protein transfer system to control operation of the one or more voltage sources.

Abstract: This application belongs to the field of biotechnology and discloses a method for producing a clam active peptide. The method for producing a clam active peptide comprises cleaning fresh clam meat with water, adding water and homogenizing with a colloid mill to prepare a clam meat slurry; adding water and complex protease for enzymolysis of the clam meat slurry, and heating to inactivate enzyme after the enzymolysis; centrifuging to collect an enzymatic hydrolyzate, capturing the enzymatic hydrolyzate having a molecular weight of lower than 2 KDa through microfiltration-ultrafiltration-nanofiltration membrane filtration, and drying to obtain the clam active peptide.

Abstract: The present disclosure provides systems and methods of electroporation protocol optimization. Embodiments include electroporation machines capable of carrying out test protocols including multiple user-designated parameters. The protocols and parameters can be carried out on samples comprising cells, including portions of a sample, to determine optimum parameters for electroporation for different samples. The systems and methods of optimization preferably use electroporation cartridges, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber and electrodes.