Abstract: An artificial non-coding RNA (ncRNA) module constructed by a synthetic biology technique and the use of the artificial ncRNA module in the construction of an artificial nitrogen fixation system are disclosed. The RNA module can enhance the post-transcriptional stability of nifHDK mRNA by interacting with a nitrogenase coding gene nifHDK mRNA, thereby improving the nitrogen fixation ability of a chassis microorganism. A fusion expression vector carrying the artificial RNA module is constructed and transformed into different chassis nitrogen-fixing microorganisms. It is confirmed through experiments that, under nitrogen fixation conditions, the artificial RNA module of the present disclosure can significantly improve the nitrogenase activity of a recombinant engineering bacterial strain.

Abstract: An artificial combined rhizosphere nitrogen fixation system includes a recombinant nitrogen-fixing engineering bacterium, which is transformed with genes for encoding a nitrogen fixation activator Neb and an ammonium transporter amtR, and a recombinant plant, which is transformed with a gene for encoding an ammonium-affiliated protein Ham. The coupling of the functions of the above two is achieved through a seed-coated inoculation at a rhizosphere of a crop.

Abstract: An artificial non-coding RNA (ncRNA) module constructed by a synthetic biology technique and the use of the artificial ncRNA module in the construction of an artificial nitrogen fixation system are disclosed. The RNA module can enhance the post-transcriptional stability of nifHDK mRNA by interacting with a nitrogenase coding gene nifHDK mRNA, thereby improving the nitrogen fixation ability of a chassis microorganism. A fusion expression vector carrying the artificial RNA module is constructed and transformed into different chassis nitrogen-fixing microorganisms. It is confirmed through experiments that, under nitrogen fixation conditions, the artificial RNA module of the present disclosure can significantly improve the nitrogenase activity of a recombinant engineering bacterial strain.

Abstract: The present disclosure discloses an artificial combined rhizosphere nitrogen fixation system, including a recombinant nitrogen-fixing engineering bacterium that is transformed with genes for encoding a nitrogen fixation activator Neb and an ammonium transporter amtR, and a recombinant plant that is transformed with a gene for encoding an ammonium-affiliated protein Ham. The coupling of the functions of the above two is achieved through a seed-coated inoculation at a rhizosphere of a crop.