Abstract: The present invention provides a method for non-invasively detecting the expression levels of miRNAs in cell media and determining the types and status of the cells, and specifically provides a method for determining the type and status of the cells according to the expression level of miRNAs in a cell medium. The method comprises: culturing different types of cells, collecting the medium of the cells, extracting RNAs from the medium, performing reverse transcription on the RNAs, detecting miRNAs in the cell medium by the fluorescent quantitative PCR method, and determining the types and status of the detected cells according to the relationship between the expression levels of miRNAs in different types and status of cells and the expression level of the detected miRNAs.

Abstract: Key genes, microRNAs, other non-coding RNAs and a combination thereof for identifying and regulating pluripotency of cells are provided. The key genes, microRNAs, other non-coding RNAs and a combination thereof are highly expressed in full pluripotent stem cells but are significantly suppressed or silenced in partially pluripotent stem cells. The genes, microRNAs and other non-coding RNAs are those of a chromosome imprinted Dlk1-Dio3 region located on the long arm of mouse chromosome 12, and are homologous genes, microRNAs and other non-coding RNAs having 70-100% homology with them in genomic syntenic regions of other mammals. Also provided are uses of the genes, microRNAs, other non-coding RNAs and combination thereof in identifying the pluripotent status of stem cells and regulating pluripotency of cells; in typing stem cells; regulating pluripotency of cells, pluripotent states and levels of cells; treating diseases; and developing drug targets for tumor treatment and antitumor drugs.