Abstract: A method for producing 2-keto-3-deoxygluconate (KDG) from 2-(acetylamino)-2-deoxy-D-gluconic acid (GlcNAc1A) by two enzymes; GlcNAc1A is converted to KDG by incubating GlcNAc1A with a deacetylase OngB at 25° C. for 4-12 h and then with a deaminase OngC at 25° C. for another 10-15 h; it constructs two engineered E. coli/pET22b-ongB (carrying the ongB gene) and E. coli/pET22b-ongC (carrying the ongC gene) strains to prepare recombinant proteins OngB and OngC, respectively; at the action of these two enzymes, OngB and OngC, GlcNAc1A is converted to KDG, which solves the bottleneck of GlcNAc1A utilization during the bioconversion of chitin; the KDG is an important metabolic intermediate to synthesize furan derivatives, herbicides, food additives and other industrially important chemical compounds, having wide industrial applications.

Abstract: MLX1034 is from Polaribacter sp. Q13, and has the amino acid sequence of the 1,3/1,4-xylanase MLX1034 is listed in SEQ ID NO.1; a nucleotide sequence of the gene is listed in SEQ ID NO.2; the 1,3/1,4-xylanase MLX1034 in the invention is capable of efficiently and specifically degrading 1,3/1,4-xylan and producing xylooligosaccharides with DP values above one; in addition, the physical and chemical properties of the 1,3/1,4-xylanase MLX1034 are stable enough to hydrolyze 1,3/1,4-xylan at room temperature; the 1,3/1,4-xylanase MLX1034 is suitable for the industrial production of red algal xylooligosaccharides at low energy costs.

Abstract: The present invention provides a breeding method for tetraploid ricinus communis. The method comprises: collecting a ricinus communis germplasm resource, performing morphologic character analysis and chromosome ploidy identification, selecting good diploid varieties, carrying out mutagenesis by using colchicine and oryzalin; performing chromosome ploidy identification on F1 generation seeds, selecting homozygous tetraploids, and performing economic character analysis and identification on the F2 generation, so as to breed good tetraploid ricinus communis.

Abstract: The present invention provides a breeding method for tetraploid ricinus communis. The method comprises: collecting a ricinus communis germplasm resource, performing morphologic character analysis and chromosome ploidy identification, selecting good diploid varieties, carrying out mutagenesis by using colchicine and oryzalin; performing chromosome ploidy identification on F1 generation seeds, selecting homozygous tetraploids, and performing economic character analysis and identification on the F2 generation, so as to breed good tetraploid ricinus communis.

Abstract: A molecular recognition sensor system is provided incorporating a molecular imprinted nanosensor device.

Abstract: A molecular recognition sensor system is provided incorporating a molecular imprinted nanosensor device.

Abstract: A molecular recognition sensor system is provided incorporating a molecular imprinted nanosensor device formed by the process steps of: (a) fabricating using photolithography a pair of metallic electrodes separated by a microscale gap onto a first electrical insulation layer formed on a substrate; (b) applying a second electrical insulation layer on most of a top surface of said pairs of electrodes; (c) depositing additional metallic electrode material onto said electrode pairs using electrochemical deposition, thereby decreasing said microgap to a nano sized gap between said electrode pairs; (d) electrochemically polymerizing in said nanogap conductive monomers containing a target analyte, thereby forming a conducting polymer nanojunction in the gap between electrode pairs; and (e) immersing resultant sensor device in a solution which removes away the target analyte, and intermittently applying a voltage to the conducting polymer while it is immersed in said solution, thereby swelling and shrinking the co

Abstract: A molecular recognition sensor system is provided incorporating a molecular imprinted nanosensor device formed by the process steps of: (a) fabricating using photolithography a pair of metallic electrodes separated by a microscale gap onto a first electrical insulation layer formed on a substrate; (b) applying a second electrical insulation layer on most of a top surface of said pairs of electrodes; (c) depositing additional metallic electrode material onto said electrode pairs using electrochemical deposition, thereby decreasing said microgap to a nano sized gap between said electrode pairs; (d) electrochemically polymerizing in said nanogap conductive monomers containing a target analyte, thereby forming a conducting polymer nanojunction in the gap between electrode pairs; and (e) immersing resultant sensor device in a solution which removes away the target analyte, and intermittently applying a voltage to the conducting polymer while it is immersed in said solution, thereby swelling and shrinking the co

Abstract: A method is provided for forming a thin film conducting polymer (24) for sensor applications. The method comprises forming at least one pair of electrodes (14, 16) on a substrate (12), the pair of electrodes (14, 16) having an insulating layer (18) positioned therebetween, the insulating layer (18) having a surface (20) opposed to the substrate (12), increasing OH? groups on the surface (20), binding silane molecules (22) to the surface (20), and forming the conducting polymer material (24) on the silane molecules (22) between and in electrical contact with the electrodes (14, 16).