Abstract: A set of primers and probes for simultaneous detection of Cymbidium mosaic virus (CymMV), Odontoglossum ringspot virus (ORSV), and Cymbidium ringspot virus (CymRSV) and a method for detecting CymMV, ORSV, and CymRSV, along with a method for their detection, are disclosed. The method involves designing multiplex real-time quantitative PCR detection primers and probes for CymMV, ORSV, and CymRSV and applying these primers and probes to the real-time quantitative PCR simultaneous detection of CymMV, ORSV, and CymRSV. It allows for faster detection of CymMV, ORSV, and CymRSV, taking only one-third of the time compared to uniplex real-time quantitative PCR technology, thereby reducing testing costs by approximately ? to ½ for each sample. The primers and probes are highly specific and sensitive, with a sensitivity as low as 1 to 10 copies. It provides an efficient and feasible detection method for early detection and prevention of CymMV, ORSV, and CymRSV.

Abstract: A method is provided for establishing an ulcerative colitis (UC) animal model and use of the model. The ulcerative colitis animal model uses Canis lupus familiaris dogs as the modeling animals and uses acetic acid to induce canine ulcerative colitis. The experimental results can be directly generalized to the human body. The modeling manner is easy to operate, establishes a stable model, is easy to reproduce, and has a low cost, which provides more animal model options for evaluation of responsiveness to drugs and therapeutic effect in research and development of UC therapeutic drugs.