Abstract: The present invention relates to the field of genetic engineering and process engineering, and discloses an engineering strain producing cordycepin and its derivative 3?-deoxyinosine, its preparation method and application. The method includes: codon optimizing genes Cm1 and Cm2; gene amplification and fragment recovering; methanol-induced promoter and terminator fragments; and inserting the genes into an expression vector and then transferring to Pichia pastoris for screening to obtain the engineering strain of Pichia pastoris of the present invention. The engineering strain can be applied to production of cordycepin and its derivative. The engineering strain prepared by the preparation method of the present invention has great advantages in producing cordycepin and its derivative.

Abstract: The present disclosure discloses a universal aptamer-based colloidal gold lateral flow test strip for detecting small-molecule substances and belongs to the fields of analytical chemistry, medicine, environment, food safety detection, nano-biosensing and the like. An AuNPs@poly-DNA probe is used for rapidly and sensitively capturing an aptamer, streptavidin sprayed in a test zone and streptavidin-biotin-DNAc sprayed in a control zone do not need to be changed, and another substance can be detected only by changing a nucleic acid chain part of the AuNPs@poly-DNA probe. A universal colloidal gold lateral flow test strip, which is rapid, sensitive and low in cost, has been developed. The test strip method for detecting small-molecule substances is simple, convenient and rapid, and can be used for detection at any time.

Abstract: An electronic control unit (ECU) assembly is provided, which includes a covering part, one or more metal oxide semiconductor field effect transistors (MOSFETs) (1), a substrate and an ECU main body. The covering part includes a lid element (3) having latch parts (6); the lid element (3) is secured onto the ECU main body via the latch parts (6); and the MOSFETs (1) are arranged between the lid element (3) and the substrate (9). The covering part further includes a middle element, which is in tight contact with and is elastically pressed against the MOSFETs (1), such that the MOSFETs (1) are brought into tight contact with the substrate. Thereby, the MOSFETs (1) are reliably fixed in the ECU assembly. A vehicle including the ECU assembly is also provided.